Expression and Immunoreactivities of Hepatitis E Virus Genotype 3 Open Reading Frame-2 (ORF-2) Recombinant Proteins Expressed in Insect Cells
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Hepatitis E virus (HEV) is a fecal-orally transmitted virus that is endemic in many geographical areas with poor sanitary conditions and inadequate water supplies. In Europe, a low-endemic area, an increased number of autochthonous sporadic human cases of patients infected with HEV strains of genotype 3, have been reported lately. The relatively high prevalence of HEV genotype 3 infections in European pigs has raised concerns about a potential zoonotic transmission to humans. Determination of HEV seroprevalence in pigs would help to clarify its incidence and possible zoonotic implications. To this purpose, we have expressed and partially characterized swine genotype 3 HEV open reading frame-2 proteins upon infection of Sf21 insect cells with recombinant baculoviruses. The use of the expressed proteins as diagnostic antigens for the detection of antibodies to HEV has been further assayed with human and swine sera.
KeywordsZoonosis Emerging virus Immunogenicity ELISA
The study was supported in part by grants (AGL2004-06071 and CSD2006-0007) from the Spanish Ministerio de Ciencia e Innovación (MICINN) to J.C.S. N.J.O. has been supported by a scholarship from Instituto Nacional de Investigación y Tecnología Agraraia y Alimentaria (INIA) and by a COST-929 short-term mission. J.A. was supported by a scholarship from the MICIIN. E.E.R. was supported by a contract ascribed to grant CSD2006-0007 from MICIIN. We are indebted to M.T. Pérez-Gracia (U. Cardenal Herrera-CEU, Valencia, Spain), M. Buti (Hospital Vall d′Hebron. Barcelona, Spain), and M. Martín (CReSA, UAB, Barcelona, Spain) for kindly providing us with swine fecal extract, human and swine sera, respectively.
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