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Apoptotic suppression of inflammatory macrophages and foam cells in vascular tissue by miR-23a

  • Rabih Roufayel
  • Seifedine KadryEmail author
Original Paper
  • 14 Downloads
Part of the following topical collections:
  1. Internet Of Medical Things In E-Health

Abstract

Recent studies have shown that microRNA-23a is down regulated in atherosclerotic tissues. In this study we sought to demonstrate that miR-23a directly binds to 3′ untranslated region of HSP90 mRNA thereby suppressing the expression of its protein. To investigate the potential roles of miR-23a in macrophage, THP-1 macrophages were transfected with miR-23a mimics or inhibitors. Our results showed inflammatory factors IL-6 and MCP-1 concentrations in cell culture medium of macrophages and foam cell transfected with miR-23a mimics were decreased. Furthermore, our findings showed that apoptosis of macrophage and foam cells transfected with miR-23a mimics were inhibited. Over expression of miR-23a in foam cells could reduce lipid intake. Meanwhile, inflammatory macrophages and foam cells transfected with miR-23a mimics, showed a significant decrease in both HSP90 and NF-κB proteins. These results demonstrate a novel finding and potential therapeutic target for atherosclerosis.

Keywords

miR-23a HSP90 Inflammatory response Apoptosis Atherosclerosis 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© IUPESM and Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Science, College of Engineering and TechnologyAmerican University of the Middle EastEqailaKuwait
  2. 2.Department of Mathematics and Computer Science, Faculty of ScienceBeirut Arab UniversityBeirutLebanon

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