RNA polymerase II ChIP-seq—a powerful and highly affordable method for studying fungal genomics and physiology
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Fungi are ubiquitous in the biosphere with an estimate of around two to five million species (Blackwell 2011; Hawksworth and Lucking 2017). This diverse group of organisms occupies most, if not all, environmental niches and plays many roles vital to our daily life (see the 2018 State of the World’s Fungi report for interesting fungal facts – https://stateoftheworldsfungi.org). One indispensable role of fungi is their contribution to the ecosystem; they are crucial in decomposing complex carbon compounds, recycling nutrients, and facilitating nutrient exchanges between organisms (Mohan et al. 2014; Treseder and Lennon 2015). There is a long history of fungi used in food production (e.g., fermentation), and some of them can be taken directly as food for nutritional or medicinal purposes (Dupont et al. 2017). Fungi are also renowned for their drug potentials with the most famous example being the antibiotic penicillin that has saved countless lives since its discovery. Analysis of...
We thank members of the Wong lab for sharing their experience with PolII ChIP-seq and Prof. Michael J. Hynes and Prof. Richard B. Todd for comments and suggestions on the manuscript.
We acknowledge the Science and Technology Development Fund of Macau S.A.R (grant number: FDCT085/2014/A2), Research Services and Knowledge Transfer Office of the University of Macau (grant numbers: MYRG2015-00186-FHS and MYRG2016-00211-FHS), and the Faculty of Health Sciences for their funding supports.
Compliance with ethical standards
Conflict of interest
Kaeling Tan declares that she has no conflict of interest. Koon Ho Wong declares that he has no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
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