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Biophysical Reviews

, Volume 10, Issue 4, pp 1201–1203 | Cite as

An historical perspective of the discovery of titin filaments –Part 2

  • Cristobal G. dos Remedios
Review

Abstract

In 2017, a Special Issue of Biophysical Reviews was devoted to “Titin and Its Binding Partners. The issue contained a review: “An historical perspective of the discovery of titin filaments” by dos Remedios and Gilmour that was intended to be a history of the discovery of the giant protein titin, previously named connectin. The review took readers back to the earliest discovery of the so-called third filament component of skeletal and cardiac muscle sarcomeres and ended in 1969. Recently, my colleague Shin’ichi Ishiwata gently reminded me of two papers published in 1990 and 1993 that were unwittingly omitted from the original historical perspective. In the first paper (J Cell Biol 110:53–62, 1990), Funatsu et al. examined the elastic filaments in skeletal muscle using a combination of light and electron microscopy, but they also measured resting as well as passive stiffness mechanical measurements to establish that connectin (titin) is responsible for both stiffness and fiber tension. In the second paper (J Cell Biol 120:711–724, 1993), Funatsu et al. used permeabilised cardiac muscle myocytes (from rabbit papillary muscles) and focussed on filament ultrastructure using either freeze-substitution or deep-etched replica methods to visualise connectin/titin filaments in fibers with and without actin and myosin filaments.

Keywords

Connectin filaments Titin filaments Skeletal muscle myocytes Cardiomyocytes Ultrastructure Sarcomeric protein 

Notes

Acknowledgements

The author is grateful for the financial assistance of Medical Advances Without Animals for the purchase of a nitrogen vapour tissue freezer. He is also grateful to Dr Shin’ichi Ishiwata for his comments on the manuscript.

Compliance with ethical standards

Conflict of interest

Cristobal G. dos Remedios declares that he has no conflicts of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by the author.

References

  1. dos Remedios C, Gilmour D (2017) An historical perspective if the discovery of titin filaments. Biophys Rev 9:179–188CrossRefPubMedPubMedCentralGoogle Scholar
  2. Funatsu T, Higuchi H, Ishiwata S (1990) Elastic filaments in skeletal muscle revealed by selective removal of thin filaments with plasma gelsolin. J Cell Biol 110:53–62CrossRefPubMedGoogle Scholar
  3. Funatsu T, Kono E, Higuchi H, Kimura S, Ishiwata S, Yoshioka T, Maruyama K, Tsukita S (1993) Elastic filaments in situ in cardiac muscle: deep etch replica analysis in combination with selective removal of actin and myosin filaments. J Cell Biol 120:711–724CrossRefPubMedGoogle Scholar
  4. Horowits R, Kempner ES, Bisher ME, Podolsky RJ (1986) A physiological role for titin and nebulin in skeletal muscle. Nature (London) 323:160–164CrossRefGoogle Scholar
  5. Kellermayer D, Smith JE 3rd, Granzier H (2017) Novex-3, the tiny titin of muscle. Biophys Rev 9:201–206.  https://doi.org/10.1007/s12551-017-0261-y CrossRefPubMedPubMedCentralGoogle Scholar
  6. Marston S, Montgiraud C, Munster AB, Copeland O, Onjee C, dos Remedios C, Messer AE, Ehler E, Knőll R (2015) OBSCN mutations associated with dilated cardiomyopathy and haploinsufficiency. PLoS One 10:e0138568.  https://doi.org/10.1371/journal.pone.0138568 CrossRefPubMedPubMedCentralGoogle Scholar
  7. Qadota H, Matsunaga Y, Nguyen KCQ, Mattheyses A, Hall DH, Benian GM (2017) High-resolution imaging of muscle attachment structures in Caenorhabditis elegant. Cytoskeleton 74:426–442.  https://doi.org/10.1002/cm.21410 CrossRefPubMedGoogle Scholar
  8. Vikhorev PG, Smolktunowicz N, Munster AB, Copeland O, Kostin S, Montgiraud C, Messer AE, Toliat MR, Li A, dos Remedios CG, Lal S, Blair CA, Campbell KS, Guilin M, Knoll R, Marston SB (2017) Abnormal contractility in human heart myofibrils from patients with dilated cardiomyopathy due to mutations in TTN and contractile protein genes. Sci Rep 7:1–11.  https://doi.org/10.1038/541598-017-13675-8 CrossRefGoogle Scholar

Copyright information

© International Union for Pure and Applied Biophysics (IUPAB) and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.The University of SydneySydneyAustralia

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