Hypertrophic cardiomyopathy and the myosin mesa: viewing an old disease in a new light
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The sarcomere is an exquisitely designed apparatus that is capable of generating force, which in the case of the heart results in the pumping of blood throughout the body. At the molecular level, an ATP-dependent interaction of myosin with actin drives the contraction and force generation of the sarcomere. Over the past six decades, work on muscle has yielded tremendous insights into the workings of the sarcomeric system. We now stand on the cusp where the acquired knowledge of how the sarcomere contracts and how that contraction is regulated can be extended to an understanding of the molecular mechanisms of sarcomeric diseases, such as hypertrophic cardiomyopathy (HCM). In this review we present a picture that combines current knowledge of the myosin mesa, the sequestered state of myosin heads on the thick filament, known as the interacting-heads motif (IHM), their possible interaction with myosin binding protein C (MyBP-C) and how these interactions can be abrogated leading to hyper-contractility, a key clinical manifestation of HCM. We discuss the structural and functional basis of the IHM state of the myosin heads and identify HCM-causing mutations that can directly impact the equilibrium between the ‘on state’ of the myosin heads (the open state) and the IHM ‘off state’. We also hypothesize a role of MyBP-C in helping to maintain myosin heads in the IHM state on the thick filament, allowing release in a graded manner upon adrenergic stimulation. By viewing clinical hyper-contractility as the result of the destabilization of the IHM state, our aim is to view an old disease in a new light.
KeywordsHypertrophic cardiomyopathy Dilated cardiomyopathy Interacting-heads motif Myosin sequestered state Myosin mesa Myosin binding protein C
In 2017 we face another commonly held belief that is taking a turn—this time regarding our understanding of the molecular basis for clinical hyper-contractility caused by hypertrophic cardiomyopathy (HCM) mutations in human β-cardiac myosin, where approximately 40% of all known HCM mutations lie (Buvoli et al. 2008; Colegrave and Peckham 2014; Harris et al. 2011; Seidman and Seidman 2001; Walsh et al. 2010). Based on studies using mouse α-cardiac myosin as a model system, significant increases in intrinsic force of the HCM mutant myosins, and increases in their ATPase activity and their velocity as measured by in vitro motility systems, seemed to easily explain the hyper-contractility seen clinically, and this view has dominated thinking in the field (Moore et al. 2012; Sivaramakrishnan et al. 2009). As explained below, however, recent results using purified human β-cardiac myosin do not fit this paradigm. Our research group and those of many others were not paying sufficient attention to another possible pivotal factor involved in HCM, namely the number of myosin heads in the sarcomere that are functionally accessible for interaction with actin (Na). As discussed below, the importance of the physiological regulation of Na in striated muscle has been discussed in studies by Padron and colleagues (Alamo et al. 2008; Brito et al. 2011) and others; so this concept is not new. As also described below, several investigators have pointed out the positions of several HCM and dilated cardiomyopathy (DCM) mutations on myosin and the roles they may play in altering Na. We have extended these ideas and have hypothesized (Adhikari et al. 2016; Kawana et al. 2017; Nag et al. 2015, 2017; Spudich 2015; Spudich et al. 2016) that increases in Na may be responsible in the majority of cases for the hyper-contractility caused by HCM mutations, both in myosin and in myosin binding protein C (MyBP-C), and this is a major subject of this review.
Muscle myosin is a hexamer consisting of two myosin heavy chains and two sets of light chains, i.e. the essential light chain (ELC) and the regulatory light chain (RLC) (Fig. 2a, c). The entire myosin molecule when proteolytically digested by chymotrypsin yields two fragments by a cut in the tail region, giving heavy meromyosin (HMM) and light meromyosin (LMM) fragments (Fig. 2a). The HMM fragment contains the two globular heads and approximately 40% of the coiled-coil tail. HMM can be further digested by papain to yield S1 and subfragment 2 (S2). S1 houses the ATP and actin binding sites followed by an α-helix that binds the ELC and the RLC. The transition between the pre-stroke and post-stroke states involves a swing of the converter domain (Fig. 2c) through an angle of approximately 70°, about a fulcrum point marked by the white star with black center in Fig. 2b. The converter swing distance is amplified by the light chain binding domain lever arm (Fig. 2b, c). The RLC can be phosphorylated, which activates the contraction of the sarcomere (Kamm and Stull 2011; Vandenboom 2016). This light chain phosphorylation turns out to be an important part of the story that we review here and is the subject of discussion further in this review.
The myosin thick filament is formed by the interaction of the LMM regions of myosin molecules packed together to form the cylindrical backbone of the bipolar thick filament. Typically, the bipolar thick filament of the human cardiac sarcomere is 1.6 μm long and 20 nm wide (Fig. 1). Myosin heads are arranged on the thick filament backbone in a helical or quasi-helical fashion. There is a 14.3-nm spacing between two adjacent myosin molecules on the filament, with a true repeat of 42.9 nm.
The major protein in the thin filament is actin, with which myosin interacts to generate contractile force. The other components of the thin filament are the Ca2+-regulatory proteins tropomyosin and troponin (troponin C, troponin I and troponin T). The thin filament Ca2+-regulation system involving tropomyosin–troponin has been thoroughly reviewed by previous authors (Brown and Cohen 2005; Gordon et al. 2000; Lehman 2016; Moore et al. 2016; Tobacman 1996; Zot and Potter 1987) and will not be discussed further here.
Another important protein of the sarcomere is titin, the main subject of the chapters in this Special Issue. Titin is a giant protein that spans half the sarcomere, with its N-terminus embedded in the Z-disk and its C-terminal region interacting with the myosin thick filament (Freiburg and Gautel 1996; Houmeida et al. 1995; Obermann et al. 1997) (Fig. 1). Titin modulates the stiffness of the muscle, with its extensible I-band region acting as a molecular spring that develops passive force when the sarcomere is stretched during diastolic filling. Other regions of the protein have been shown to participate in force-dependent signaling (Linke 2008). HCM mutations are very rare in titin, limited primarily to missense mutations that increase titin’s interactions with binding partners, such as alpha-actinin and four and a half LIM protein 2 (FHL2) (Matsumoto et al. 2005; Satoh et al. 1999). In contrast, 30% of patients with DCM carry mutations in the titin gene (Herman et al. 2012), mostly localized to the A-band region (Fig. 1a). A fundamental difference between HCM and DCM is that HCM results in clinical hyper-contractility while DCM results in hypo-contractility. Mutations that cause defects in the contractile apparatus are intellectually easy to understand—if you do anything to break the system, you will observe hypo-contractility. Hyper-contractility caused by HCM mutations, however, is intellectually a less obvious circumstance—these mutations cause the contractile apparatus to produce higher power output than normal! How this may be explained mechanistically is one focus of this review. Here we emphasize regulation of the thick filament system in striated muscle, primarily cardiac muscle, with a focus on a conformation of myosin that has its S1 heads folded back on their own S2 tail and interacting with each other. This state has been referred to as the interacting-heads motif (IHM) (Alamo et al. 2008). The heads in the IHM are said to be in an ‘off state’, since they are not accessible for interaction with actin. We argue that a majority of myosin HCM mutations, and possibly most MyBP-C mutations as well, likely weaken this IHM state, resulting in more functionally accessible myosin heads for actin interaction, thus causing the hyper-contractility observed for HCM clinically. Rather than simply describe the current status of thinking in the field, we make an effort to clarify what is known, what is speculative and what needs to be done going forward.
Power output by the sarcomere is the ensemble force of the system times the velocity of contraction
Each head of myosin is an individual force generator and can produce its own intrinsic force (Fintrinsic). Na is the number of myosin heads on the thick filament that are ‘functionally accessible’ for a fruitful interaction with actin. The duty ratio is a term that reflects the fraction of those accessible myosin heads that are bound to actin in a strongly bound, force-generating state at any moment during contraction. It is the ratio of the strongly bound state time (ts) divided by the total cycle time (tc) of the actin-activated myosin chemomechanical cycle (Fig. 2b). For cardiac muscle under some load, the duty ratio is approximately 0.2 (depicted in Fig. 1). As discussed below and depicted in Fig. 1, Na is not all of the myosin heads that are physically present in the sarcomere, but may be as low as only 50% of those heads (Brito et al. 2011). It’s the proposed approximately 50% of heads that are not functionally accessible for interaction with actin that offers exciting new revelations in muscle research—and is the focus of this review.
The myosin mesa and a possible unifying hypothesis for the clinical hyper-contractility caused by HCM mutations
Hundreds of missense mutations in both β-cardiac myosin and MyBP-C are responsible for the devastating inherited disease hypertrophic cardiomyopathy. As already described, approximately 40% of the mutations leading to HCM are found in β-cardiac myosin itself and another 40% in MyBP-C. Those numbers, derived from human genetics studies alone, tell us that these two proteins in particular are somehow speaking to each other to regulate contractility and that mutations in either result in hyper-contractility clinically.
Genetically-based HCM, an autosomal dominant inherited disease (Geisterfer-Lowrance et al. 1990; Seidman and Seidman 2000, 2001), is clinically characterized by hypertrophy of the ventricular walls with a resultant decrease in ventricular chamber size. Systolic performance of the heart is preserved or even increased, but relaxation capacity is diminished. HCM affects more than one in 500 individuals (Harvey and Leinwand 2011; Maron 2010; Maron et al. 1995; Semsarian et al. 2015) and is the most common cause of sudden cardiac death in young adults (Harvey and Leinwand 2011). Current treatment for HCM is limited to symptomatic relief and includes heart muscle reduction surgery (myectomy), defibrillator placement and even heart transplant in the most refractory cases. There is an urgent need for new therapeutic approaches to the disease, but first we need to fully understand its underlying molecular basis. To do so, it is important to reconstitute the important elements of the sarcomere from purified proteins using human cardiac isoforms.
The importance of using purified, homogeneous human cardiac isoforms is emphasized by the variable results obtained using model systems and human biopsy samples. Many studies, largely using the mouse ventricular α-cardiac myosin as a model, have contributed to our current understanding of the effects of some HCM mutations on various functions of the purified actin–myosin complex, usually its ATPase activity or its velocity in the in vitro motility assay (for review, see Moore et al. 2012; Sivaramakrishnan et al. 2009). Although several cardiomyopathy mutations in β-cardiac myosin have been studied over the past 15 years, there has been significant disagreement expressed in the literature regarding the effects of these mutations on the biochemical and biophysical properties of cardiac myosins (reviewed in Moore et al. 2012).
The first HCM-causing mutation in β-cardiac myosin to be identified was R403Q (Geisterfer-Lowrance et al. 1990). Initial studies on this mutant isolated from human soleus muscle biopsies reported a decrease in in vitro sliding velocity (Cuda et al. 1997), while later studies using human cardiac biopsy protein found an increase in sliding velocity (Palmiter et al. 2000). Studies using mouse α-cardiac myosin containing the R403Q mutation found an increase in velocity and also an increase in ATPase activity (Tyska et al. 2000). Lowey et al. (2008) subsequently showed that the effects of the R403Q mutation in mouse cardiac myosin depended on the isoform into which the mutation was introduced. In α-cardiac myosin, these authors saw an increase in both ATPase activity and velocity, whereas in the β-cardiac myosin, they observed no significant change in the velocity and actually a slight decrease in the ATPase activity (Lowey et al. 2008).
According to Eq. 1, increases in intrinsic force, velocity or ATPase activity will lead to the hyper-contractile phenotype at the molecular level, and this has been the general view of the molecular basis of hyper-contractility caused by HCM mutations. Recent work carried out on two early-onset HCM mutations using recombinant human β-cardiac myosin S1 indeed shows that all these parameters are significantly increased (25–90%) (Adhikari et al. 2016). However, the same experiments carried out on five typically adult-onset HCM mutations, including R403Q, using the same human β-cardiac myosin S1 construct, showed variable results with only modest changes compared to wild-type (WT) myosin (Kawana et al. 2017; Nag et al. 2015; Sommese et al. 2013). Moreover, some of these small changes contributed to hyper-contractility, others contributed to hypo-contractility, and some showed no change, making it difficult to assess the net effect of these mutations on power. It became clear that something was missing, and as described below, we believe that a primary effect of many HCM mutations in both myosin and MyBP-C is an increase in Na, resulting in more heads becoming functionally accessible for interaction with actin and offering a better explanation for the hyper-contractility observed clinically in the majority of adult onset HCM patients.
In order to map the known HCM mutations that are clearly pathogenic on the human β-cardiac myosin molecule we created homology models of the pre-stroke and post-stroke states of human β-cardiac myosin S1, with the full sequence of the human β-cardiac S1 heavy chain and the human ventricular ELC and RLC (Spudich 2015 ; Homburger et al. 2016). The templates used for the homology modeling were the crystal structure of the human β-cardiac myosin motor domain obtained by Winkelman et al. (2015) and the crystal structures of the lever arm containing the squid ELC and RLC. These homology models (HBCprestrokeS1.pdb and HBCpoststrokeS1.pdb) are available for download from our website (http://spudlab.stanford.edu/homology-models/).
A list of residues forming the mesa, the actin-binding interface, and the blocked head interface that binds the converter of the free head are shown. The colored residues are HCM mutations
A folded-back state of myosin known as the interacting-head motif is tailor-made for regulation of Na
The IHM structure has been notable in images of intact thick filaments isolated from a variety of types of muscle. Thus, low-resolution thick filament structures have been observed in EM studies of tarantula skeletal muscle (Woodhead et al. 2005), Limulus (Zhao et al. 2009), scallop striated muscle (Woodhead et al. 2013), scorpion skeletal muscle (Pinto et al. 2012), zebrafish cardiac muscle (Gonzalez-Sola et al. 2014), Schistosoma mansoni smooth muscle (Sulbaran et al. 2015) and vertebrate cardiac muscle (Al-Khayat et al. 2013; Zoghbi et al. 2008). A perpendicular-type of IHM has been reported for Lethocerus indirect flight muscle (Hu et al. 2016). The highest resolution achieved to date for the region of myosin heads is 2.0 nm for the conventional parallel-type of IHM in tarantula (Alamo et al. 2008) and 2.0 nm for the conventional perpendicular-type IHM in Lethocerus (Hu et al. 2016).
Three-dimensional reconstruction of the electron density maps from isolated thick filaments confirmed the presence of a helical array of myosin heads, also known as crowns, along the circumference of the thick filament. The crowns are separated by an approximately 14.5-nm axial repeat with a twist of 30° in the tarantula thick filament (Woodhead et al. 2005). Three successive crowns form the basic repeating unit in the helical array of heads in the thick filament. In cardiac thick filaments, three crowns within a 42.9-nm repeat are arranged in a perturbed helical fashion. Inter-crown distances have been observed to be different than 14.3 nm in between certain crowns (Al-Khayat et al. 2013). Furthermore, there is a deviation in azimuthal position from an ideal helical array. Additionally, extra densities, possibly corresponding to the positions of titin and MyBP-C, were described in a mouse cardiac muscle thick filament reconstruction (Zoghbi et al. 2008) and later in a human cardiac thick filament reconstruction (Al-Khayat et al. 2013). According to the model of Al-Khayat et al. (2013), the human cardiac thick filament has 11 domains of titin in every 42.9-nm repeat where two pairs of such titin molecules are present adjacent to three crowns, and the three C-terminal domains of MyBP-C lie close to one of the three crowns. Two of the three crowns of the 42.9-nm repeat in the cardiac thick filament have the myosin heads interacting asymmetrically as in the folded state, whereas the third one has a relatively mobile conformation (Al-Khayat et al. 2013; Zoghbi et al. 2008). This mobile conformation may relate to the swaying head concept proposed by Brito et al. (2011) on the basis of structural considerations which suggested that the free heads are less strongly bound to the filament backbone and may oscillate occasionally between the attached and detached states (swaying heads).
Atomic fitting of myosin heads into the 3D reconstruction of relaxed tarantula thick filaments suggests that intramolecular head–head interactions are present within the crowns as well as intermolecular interactions (Woodhead et al. 2005; Alamo et al. 2008, 2016). Intermolecular interactions between heads of adjacent crowns has been observed in a variety of thick filament types (Alamo et al. 2008; Al-Khayat et al. 2013; Gonzalez-Sola et al. 2014; Pinto et al. 2012; Sulbarán et al. 2015; Woodhead et al. 2005; Zhao et al. 2009; Zoghbi et al. 2008).
To summarize to this point, Alamo et al. (2008) has reported the highest resolution (2.0 nm) 3D reconstruction achieved to date for the IHM structure of frozen-hydrated thick filaments (tarantula), and later studies from that group (Alamo et al. 2016) based on that 3D reconstruction have been very useful for understanding not only tarantula thick filament structure and function, but they have also had important implications for other invertebrate and vertebrate skeletal muscles. While higher average resolutions of 1.3 and 0.6 nm have been achieved for the tarantula (Yang et al. 2016) and Lethocerus thick filaments (Hu et al. 2016), respectively, their actual resolutions in the region of the myosin IHMs unfortunately are still 2.0 nm. The prospect of increasing this limiting resolution of 2 nm on the head regions of the thick filament is not promising since the disorder stemming from the free swaying heads is intrinsically reflected after the rapid freezing step (Yang et al. 2016). Although it is possible that the swaying heads could be locked down by treatment with, for example, blebbistatin, which stabilizes the IHM (Zhao et al. 2009), as already mentioned, not all IHM structures in the thick filament are identical, which severely limits the resolution that can be obtained. A higher resolution structure of the IHM per se is more likely to come from purified myosins containing two heads and the proximal S2, again stabilized in some way in the IHM state. Thus, while admittedly difficult, one future direction that is urgently needed in this field is to obtain a true atomic-resolution structure of the IHM, either by high-resolution EM or by X-ray crystallography. From the point of view of the effects of HCM mutations on the IHM structure, the high-resolution structure of the human β-cardiac myosin IHM is needed.
A step in this direction is the work of Blankenfeldt et al. (2006). These investigators solved the crystal structure of the proximal S2 region of human β-cardiac myosin, pointing out three distinct clusters or rings, Rings 1, 2 and 3, of negative charge along the proximal S2 interrupted by zones of neutral or moderately positive character. They fitted the proximal S2 crystal structure to the tarantula IHM structure of Woodhead et al. (2005) and drew attention to potential interactions between the blocked head and the S2 Ring 1 that possibly involve the positively charged loop 2 of the blocked head and the free head and the S2 Ring 2. These authors further showed that the disordered nature of the head–rod junction is important for the formation of the IHM folded structure.
The IHM state is regulated by RLC phosphorylation
It has long been known that phosphorylation–dephosphorylation of the RLC of smooth muscle myosin is an on–off switch for smooth muscle myosin activity (Lowey and Trybus 2010). This regulation has been observed at the molecular level by monitoring the catalytic efficiency of myosin as a function of phosphorylation of the RLC. Smooth muscle RLC phosphorylation has been shown to release the myosin heads from the folded-back IHM state into an ‘on state’ available for actin interaction (Wendt et al. 1999). The dephosphorylated smooth muscle myosin has very low actin-activated ATPase activity and cannot translocate actin in an in vitro motility assay (Trybus et al. 1997). Phosphorylation of even one of the heads of smooth muscle HMM can lead to a functional motor (Rovner et al. 2006; Walcott et al. 2009). Single-headed smooth muscle HMM does not retain the regulation of the two-headed construct, indicating that the folded state IHM requires the interaction of both of the heads of myosin (Cremo et al. 1995, 2001).
Although striated muscle myosins are not regulated in an on–off fashion by RLC phosphorylation, it is clear that RLC phosphorylation does affect their structure and function. For example, the myosin heads of skinned rabbit skeletal muscle fibers become more mobile when the RLC is phosphorylated at its serine 15 residue by the calmodulin-myosin light chain kinase (MLCK) complex in the presence of Ca2+ (Levine et al. 1996). RLC phosphorylation also has an impact on the activity of cardiac muscle myosin. RLC phosphorylation is important for normal heart function, and dephosphorylation of RLC can lead to hypertrophy of the heart (Huang et al. 2008). In the human cardiac sarcomere, approximately 40% of the RLCs are estimated to be phosphorylated at Ser-15 by balanced activities of MLCK and myosin light chain phosphatase (Toepfer et al. 2013). In rat cardiac trabeculae, RLC phosphorylation increases the velocity of shortening of the muscle, isometric force production and power generation (Toepfer et al. 2013). Polarized fluorescence intensity measurements in ventricular trabeculae of the rat heart have also revealed that phosphorylation of the RLC shifts the equilibrium between folded and open states toward more open heads of myosin (Kampourakis and Irving 2015).
Important findings on the role of phosphorylation and the control of the number of myosin heads that are functionally available for interaction with actin (Na; Eq. 1) come from studies of tarantula skeletal myosin (Alamo et al. 2008, 2015; Brito et al. 2011; Craig et al. 1987; Espinoza-Fonseca et al. 2015; Padron et al. 1991; Sulbaran et al. 2013). As early as 1987, Craig, Padron and Kendrick-Jones (Craig et al. 1987) showed that phosphorylation of tarantula skeletal myosin is accompanied by potentiation of the actin activation of the myosin ATPase activity and by the loss of order of the helical crossbridge arrangement characteristic of the IHM state in relaxed tarantula thick filaments. These authors suggested that in the relaxed state, when the RLC are not phosphorylated, the myosin heads are held down on the filament backbone in what is now considered to be the IHM state and that phosphorylation of the RLC cause the crossbridges to become more loosely associated, with the filament backbone giving rise to the observed changes and facilitating crossbridge interaction with actin. Then in 1991, Padron and colleagues (Padron et al. 1991) showed changes in the equatorial X-ray diffraction patterns of tarantula muscles in the phosphorylated state that supported the movement of myosin heads away from the backbone of the filament. The results of further study by these same authors led to their suggesting that the structural differences between the blocked and free heads in the IHM could preset the order in which the two heads are released upon phosphorylation (Alamo et al. 2008). The free head would be the one conformationally and properly located to be released first to interact with actin, followed by the blocked head (Alamo et al. 2008; Brito et al. 2011). Thus, the free heads were suggested to be less strongly bound to the filament backbone and may oscillate occasionally between the attached and detached states (swaying heads). It was postulated that once MLCK becomes activated, it phosphorylates free heads, which then renders the free heads fully mobile, exposing the blocked head phosphorylation site to MLCK. This would release the blocked heads, allowing their interaction with actin. According to this model, twitch force would be produced by the rapid interaction of swaying free heads with activated thin filaments, although it should be noted that for the free heads to be able to interact with actin, the S2 would have to be released from the shaft of the thick filament to allow the heads to reach the actin. Further experimental evidence supporting the presence of swaying heads and a cooperative phosphorylation mechanism for activation of tarantula thick filaments were presented in subsequent papers on the tarantula IHM state (Alamo et al. 2015, 2016; Espinoza-Fonseca et al. 2015; Sulbaran et al. 2013; for review, see Vandenboom 2016). With respect to the focus of the present review, which is the cardiac system, it will be very important to examine the details of RLC phosphorylation control of the cardiac sarcomere to see if it is the same as that in the tarantula skeletal muscle.
A beginning of such studies on the cardiac system is the functional effect of RLC phosphorylation using two-headed human β-cardiac myosin with two different lengths of the coiled-coil S2 tail region (Nag et al. 2017). The two HMM constructs, i.e. 2-hep HMM and 25-hep HMM, have two heptad repeats and 25 heptad repeats of the S2 tail region, respectively. 25-hep HMM shows an inhibition of its ATPase of approximately 40% when its RLCs are de-phosphorylated. In contrast, 2-hep HMM shows no effect of the de-phosphorylation of Ser-15 of human cardiac RLC on the maximal ATPase rate in actin-activated ATPase assays, indicating that head–tail interactions lower activity in the 25-hep construct. Importantly, the phosphorylation of RLCs in 25-hep HMM leads to a maximal activity (kcat of approx. 2.5 s−1) that is the same as that of the 2-hep HMM (kcat of approx. 2.5 s−1) and of a truncated form of human β-cardiac myosin S1 lacking the RLC (short S1, or sS1; kcat of approx. 2.5 s−1) (Adhikari et al. 2016; Kawana et al. 2017), where head–tail interaction is not possible. This result fits well with the results of studies of Trybus et al. (1997) who showed that the minimal size at which smooth muscle myosin molecules are capable of regulation via light chain phosphorylation includes the presence of a length of S2 approximately equal to the size of the myosin head. These authors concluded that the myosin S2 mediates specific interactions with the head that are required to obtain the completely inactive state of smooth muscle myosin.
In addition to demonstrating control of contractility by RLC phosphorylation, it has been shown that mechanical force applied on skeletal myosin thick filaments can regulate the number of active heads (Linari et al. 2015). As the load on thick filaments increases, more heads are liberated from the folded IHM state, resulting in an increase in the number of active heads. A similar mechanism was also shown to operate in cardiac muscle wherein during transition from diastole to systole, the number of myosin motors recruited from the IHM state are related to the systolic force, which is in turn dependent on the sarcomere length (Reconditi et al. 2017; Zhang et al. 2017). Reconditi et al. (2017) reveal that in an intact cell at rest, most myosin motors are in an IHM ‘off state’ and that the number of myosin motors switching on increases with the stress on the thick filament. This study also hints at a possible link between HCM-causing mutations which affect the intra-and intermolecular interactions that hold the IHM state of myosin motors and the hyper-contractility observed in HCM patients. A loss in the stress-sensing mechanism of the thick filament by mutations in myosin or myosin binding protein C can adversely affect the stability of the IHM state of myosin motors. On the other hand, it has also been shown that sarcomere length-dependent structural changes in the thick and thin filaments are related to the passive force generated by titin (Ait-Mou et al. 2016; Zhang et al. 2017). Based on this concept, it can be envisioned why some titin mutations tend to cause DCM. A number of missense mutations would decrease the stiffness of titin, which could result in the loss of titin’s ability to activate the thick filament and in turn lead to a reduction in the number of functionally accessible myosin heads and ultimately to DCM.
Myosin binding protein C may be involved in the IHM state
MyBP-C is a linear polymer localized along the myosin thick filament in muscle
The location of MyBP-C in the thick filament was first revealed by immuno-EM, visible as eight or nine transverse stripes (C-zone) in each half of the A-band (Craig and Offer 1976; Lee et al. 2015; Luther et al. 2008) (Fig. 1). These stripes are approximately 43 nm apart, the same as the true repeat distance between myosins along the thick filament. MyBP-C is a structural component of the thick filament and expressed as three isoforms in human muscle: the fast skeletal isoform, the slow skeletal isoform and the cardiac isoform. The cardiac MyBP-C is a 140-kDa protein, with a length and diameter of approximately 40 and 2–3 nm, respectively, consisting of 11 subdomains, eight of which belong to the immunoglobulin family (C0–C5, C8 and C10) and three to the fibronectin type-III family (C6, C7, C9), plus a proline–alanine-rich domain (PA) between the C0 and C1 domains and a phosphorylatable M domain between the C1 and C2 domains (Fig. 3). MyBP-C is thought to help in the regular organization of the thick filaments.
The cardiac isoform when compared to the skeletal isoform has characteristic structural additions which may hint at a special role of the cardiac MyBP-C. These additions are the globular C0 domain at the N-terminus, the phosphorylatable M-domain and a 28 amino acid-charged loop added to the C5 domain (Flashman et al. 2004). The phosphorylatable motif, referred to as the M-domain, has four serines that can be phosphorylated upon adrenergic stimulation (Fig. 3). The M-domain is a target of protein kinase A (PKA) (Gautel et al. 1995; Mohamed et al. 1998), protein kinase C (Mohamed et al. 1998; Xiao et al. 2007), protein kinase D (Bardswell et al. 2010; Dirkx et al. 2012), p90 ribosomal S6 kinase (Cuello et al. 2011) and Ca+2/calmodulin-dependent kinase II (Gautel et al. 1995; Sadayappan et al. 2011). Additionally, the flexible proline/alanine-rich linker called the PA loop also exists in cardiac MyBP-C between the C0 and C1 domains. For the remainder of this review, the term MyBP-C is used to refer to the cardiac isoform.
There have been reports that sequence differences of the PA loop between various species can modulate the actin–myosin interaction and that the percentage of prolines and alanines in the PA loop are inversely proportional to the heart rate (Shaffer et al. 2010). The PA loop is also rich in negatively charged residues, which may hint at a role of the loop in acting as a binding interface. Indeed, a recent study by Colson et al. (2016) demonstrated that phosphorylation-dependent allosteric changes do propagate between the M-domain and the PA loop.
MyBP-C has a number of post-translational modifications apart from phosphorylation, including acetylation (Ge et al. 2009; Govindan et al. 2012), S-glutathionylation (Patel et al. 2013), citrullination (Fert-Bober and Sokolove 2014), carbonylation (Aryal et al. 2014) and S-nitrosylation (Kohr et al. 2011). The roles of these post-translational modifications are not known and is an active area of research. Recent evidence suggests that Ca2+ induces structural changes in MyBP-C that override the impact of phosphorylation (Previs et al. 2015, 2016). These studies, performed with isolated N-terminal fragments of MyBP-C, show that the phosphorylation effects of MyBP-C are maximum when the Ca2+ levels are low. There is also evidence that the M-domain of MyBP-C can bind calmodulin in a Ca2+-dependent manner and that it may represent a structural connector between Ca2+, phosphorylation and other signaling pathways (Lu et al. 2012; Michie et al. 2016).
MyBP-C has been shown to interact with both actin and myosin and is implicated in several regulatory functions
Myosin binding protein C is tightly bound to the myosin thick filament backbone or the LMM region by way of its C-terminus (C8–C10) (Flashman et al. 2007; Miyamoto et al. 1999). The C-terminus also interacts with titin in the thick filament (Freiburg and Gautel 1996). The C0–C7 region of MyBP-C is thought to extend out from the thick filament, and there is evidence supporting its modulation of contraction by interaction with both myosin and actin filaments (Kampourakis et al. 2014). The N-terminal domain of MyBP-C (C0–C2) binds to the regulated actin filament, where it is thought to activate contraction by facilitating the movement of tropomyosin away from its blocked state for myosin binding (Mun et al. 2014). A considerable amount of work has been devoted to the interaction of the N-terminal domain of MyBP-C with the thin filament (Kulikovskaya et al. 2003; Rybakova et al. 2011; Shaffer et al. 2009; Squire et al. 2003; Weith et al. 2012), and this aspect of muscle biology has been the focus of recent reviews (Craig et al. 2014; van Dijk et al. 2014).
It has been suggested that the N-terminal region of MyBP-C might switch positions between actin and myosin binding as a regulatory mechanism (Kampourakis et al. 2014; Moss et al. 2015). The interactions of C0–C2 with myosin are thought to inhibit the function of the myosin heads, and HCM mutations have been suggested to affect this regulation (Ababou et al. 2007, 2008; Gruen and Gautel 1999; Gruen et al. 1999; Ratti et al. 2011). By characterizing the effects of the C1–C2 MyBP-C fragment on the structures of the thin and thick filaments in contracting heart muscle cells, Kampourakis et al. (2014) showed that MyBP-C stabilizes the ‘on state’ of thin filaments and the ‘off state’ of thick filaments. Their results are consistent with a model for the control of heart muscle contraction in which the regulatory functions of the thin and thick filaments are coordinated by MyBP-C.
As shown recently, human de-phosphorylated cardiac MyBP-C binds directly to human β-cardiac short S1 (sS1), which lacks the RLC, with a Kd of approximately 15–20 μM, and phosphorylation of the MyBP-C with PKA reduces the affinity of the binding significantly (Nag et al. 2017). Similar but somewhat weaker binding (Kd = approx. 35 μM) was seen with the de-phosphorylated C0–C2 domain alone. This was the first experimental evidence of full-length MyBP-C or the N-terminal fragment of MyBP-C binding the sS1 region of myosin in a phosphorylation-dependent manner, and it suggests that MyBP-C can bind both the S1 and S2 regions of myosin and thus possibly hold the heads in the IHM state on the thick filament (Fig. 6). The equilibrium between sequestered and free heads could then be shifted toward free heads upon phosphorylation of the MyBP-C.
Transgenic mouse studies have yielded invaluable insights into the workings of MyBP-C. Targeted knockout (MyBP-C −/−) mice do not show embryonic lethality, but develop severe cardiac hypertrophy at around 3 weeks after birth. These hearts have myocyte disarray and increased fibrosis, typical signs of HCM (Carrier et al. 2004; Cazorla et al. 2006; Harris et al. 2002). Fiber studies from these mice demonstrated that they had increased crossbridge cycling rates and increased power output (Korte et al. 2003; Stelzer et al. 2006a, b). Structural data based on low-angle X-ray scattering and EM studies also revealed that the homozygous knockout mice have myosin heads that are more disordered than those of the WT mouse and that the myosin heads move out azimuthally away from the thick filament surface upon loss of MyBP-C (Colson et al. 2007). A very recent EM study (Kensler et al. 2017) imaged isolated thick filaments from transgenic mouse hearts that had three of the phosphorylatable serine residues of the M-domain replaced either with alanine or aspartic acid to mimic the non-phosphorylated or the fully phosphorylated state of MyBP-C, respectively. This work demonstrated that the thick filaments from the phospho-deficient hearts had highly ordered presumed ‘off-state’ myosin heads, while the thick filaments from phosphomimetic hearts had more disordered myosin heads, consistent with their switch to an ‘on state’ for interaction with actin. All these data support the notion that MyBP-C modulates the actin–myosin interaction partly through interaction of MyBP-C with myosin by tethering myosin heads to the thick filament, and that this tethering is weakened upon the phosphorylation of MyBP-C.
Further studies are required in all areas of MyBP-C regulation of contraction. Particular attention needs to be given to the roles of the central C3–C7 domains, especially in the context of the novel 28 amino acid insertion in the C5 domain that is specific to cardiac MyBP-C. Of critical importance is a high-resolution cryo-EM-based or X-ray crystallographic structure of the entire MyBP-C bound to myosin. Such a structure will yield essential insights into the nature of molecular interactions in the proposed IHM complex.
A super-relaxed state of myosin has been postulated to be related to the IHM state
Cooke and colleagues discovered the existence of a novel state of myosin molecules in striated muscle fibers which they termed the super-relaxed state (SRX). This state was initially discovered in rabbit skeletal muscle and thereafter also seen in rabbit cardiac, tarantula skeletal and mouse cardiac fibers (Hooijman et al. 2011; McNamara et al. 2016; Naber et al. 2011; Stewart et al. 2010). The hallmark of this state is that myosin molecules in the SRX state have an extremely slow release of nucleotide from the active site. An appealing hypothesis is that the SRX state is related to the IHM state seen in EM studies (Alamo et al. 2016; Cooke 2011; Hooijman et al. 2011; Nogara et al. 2016b; Wilson et al. 2014), as discussed in more detail below.
Thus, myosin is thought to exist in three states in the sarcomere. The first is the active cycling state of myosin which interacts with the thin filament and has a rapid ATP turnover time of <1 s. The second state, often called the disordered relaxed myosin, has an intermediate ATP turnover time of <30 s. In this state the myosin is detached from actin and cycles ATP at a basal rate. The third, or SRX state, is the state which has very slow ATP turnover times of more than 100 s. In cardiac sarcomeres the approximate ATP turnover time in the SRX state is approximately 140 s per myosin (Hooijman et al. 2011).
There is a key difference between the SRX of skeletal and cardiac sarcomeres. In the skeletal fiber, upon activation by Ca2+, the SRX state is completely abolished, while in the cardiac fiber even after activation the slow phase of ATP turnover is still observed (Hooijman et al. 2011; Stewart et al. 2010). This hints at a possible fundamental difference in the allosteric communication that occurs between different myosin molecules within a skeletal versus a cardiac thick filament. In the skeletal sarcomere, it has been shown that a few tightly actin-bound heads of myosin can generate thick filament stress which in turn activates heads out from the SRX into the disordered state which are then recruited for contraction (Linari et al. 2015). This cooperative mechanism may explain the disappearance of SRX upon activation of skeletal fibers, but such a mechanism may not operate in cardiac muscle. The skeletal muscle needs to generate instantaneous force upon activation, while cardiac muscles need to undergo repetitive cycles of contraction and relaxation during every heartbeat. Thus, in the case of the heart, the SRX may provide a cardioprotective role by sequestering myosin heads and thus minimizing the energy usage of the heart, all the while keeping an important reserve of heads available when needed (Hooijman et al. 2011; McNamara et al. 2015).
It is important to note that there have been no studies in vertebrate striated fibers that directly demonstrate any structural corollary of the SRX state. Cooke and his colleagues (Alamo et al. 2016; Nogara et al. 2016b) have made the reasonable and appealing proposal that the SRX is related to the folded-back sequestered IHM state seen by others in EM studies. This relationship seems highly likely, but SRX has only been described biochemically in skinned striated muscle fibers, where other proteins associated with the thick filament could easily be playing a role in reducing basal level ATPase rates to the SRX level. Alamo et al. (2016) have hypothesized that conserved intramolecular interactions that maintain the myosin IHM state can explain the structural basis of the tarantula muscle super-relaxed state. This hypothesis needs to be tested biochemically, however, since detailed molecular interactions in the IHM remain hypothetical with a 2-nm resolution map and will have to await a high-resolution (3 Å or better) structure of the IHM. Data are available on purified de-phosphorylated smooth muscle HMM, showing a very slow ATPase (<0.004 s−1) (Cremo et al. 1995), but phosphorylation/dephosphorylation is an on/off switch for smooth muscle, unlike striated muscle. A recent study by Rohde et al. (2017) demonstrated a marginal increase in the basal ATPase of bovine cardiac HMM at 25 mM KCl versus 0 mM KCl which may hint at disruption of the IHM by increased ionic strength. However, this experiment needs to be repeated as a function of phosphorylation of the RLC. There are no other data showing a large reduction in basal ATPase with purified skeletal or cardiac myosin in a folded-back configuration. It is very important, therefore, to demonstrate biochemically with purified human β-cardiac myosin (or HMM) whether de-phosphorylated myosin alone results in a decrease of basal ATPase to the levels seen in the SRX in fibers. If not, then the question is which proteins need to be added (MyBP-C, titin, others) to the myosin to reduce the ATPase activity to the low SRX values. A recent study exploring the impact of homozygous and heterozygous MyBP-C knockout mice on the population of the SRX state revealed that the homozygous knockout of MyBP-C significantly decreased the proportion of myosin heads in the SRX state, as compared to the WT (McNamara et al. 2016), implicating MyBP-C in SRX in vertebrate striated muscle. However, the SRX state has been demonstrated biochemically in tarantula skeletal muscle (Naber et al. 2011), which does not contain MyBP-C. Thus, MyBP-C per se is not necessary for the SRX state in all systems.
A primary effect of HCM mutations may be to increase Na by reducing the levels of the IHM state in cardiac muscle
Several putative HCM mutations have been mapped on the tarantula IHM, and these cardiomyopathy mutations map to regions that could potentially alter the IHM structure (Alamo et al. 2008; Blankenfeldt et al. 2006; Moore et al. 2012; Waldmuller et al. 2011). The first of such mappings was done on proximal S2 by Blankenfeldt et al. (2006) using the tarantula IHM structure of Woodhead et al. (2005). These authors showed that the HCM mutations R869C or R870H probably affect the structural integrity of proximal S2 by disrupting side-chain hydrogen-bonding networks, while several others lead to charge inversion and cluster within the second negative charge belt (Ring 2) centered around residue E927. Comparison of the structures of WT- and E924K-proximal S2 demonstrates that these mutations are unlikely to have a structural effect (Blankenfeldt et al. 2006). Instead, the HCM mutation E924K has been shown to lead to a loss of binding of the C1–C2 fragment of MyBP-C (Gruen and Gautel 1999), implicating the second negative charge belt in S2 (Ring 2) as the interaction partner of C1–C2. Gruen and Gautel (1999) pointed out that the charge-inverting HCM mutations E927K, E930K and E935K could have a similar effect and that electrostatic interactions may play a role in the regulatory function of cardiac MyBP-C. Subsequently, Alamo et al. (2008) mapped five HCM residues on the tarantula IHM structure and suggested that the close proximity and possible interaction of four proximal S2 mutations (E924K, E927K, E930K, and E935K) with the myosin loop containing R403Q (called the ‘cardiomyopathy loop’) may be helpful in understanding how mutations in the rod region of human cardiac myosin lead to disease. This assignment was corrected, however, in a later IHM model by the same group (Alamo et al. 2016), who then suggested that it is myosin loop 2, not the cardiomyopathy loop, that is responsible for the electrostatic docking interaction with proximal S2. The mapping of seven putative HCM mutations on the motor domain to the tarantula IHM was also reported by Waldmuller et al. (2011), who concluded that the mutations they studied do not correspond to distinct myosin motor domains and suggested a random distribution of mutations in the molecule. However, it is now clear that HCM mutations fall in hotspots, as described above. Finally, Moore et al. (2012) mapped five potentially interacting residues to a tarantula IHM structure and pointed out that HCM mutations lie near these interacting sites; these authors suggested that these cardiomyopathy mutations could potentially alter the interacting head motif. One cautionary note here is that one must be careful in describing which putative HCM mutations are clearly causative of HCM because in many cases HCM mutations are reported from a single individual or from an insufficient number of individuals to be certain that the ‘HCM mutation’ is not simply a normal variant in the population. The inclusion of possible benign ‘HCM mutations’ in structural analyses can easily mislead the spatial analysis and potential clustering of disease-causing HCM mutations on the molecule.
To tie together the mesa hypothesis with the known IHM structure, we asked the question of just where on the IHM structure are the mesa surfaces of the S1 heads? To explore this, we performed homology modeling of three IHM versions of human β-cardiac myosin using the tarantula IHM structures of Alamo et al. (2008) as templates. Two versions (MS01, MS02) (Nag et al. 2017) used Protein Data Bank (PDB) 3DTP (Alamo et al. 2008) as the template and the third (MS03) (Nag et al. 2017) used PDB 3JBH (Alamo et al. 2016) as the template. These were all energy minimized using the YASARA (Krieger et al. 2009) force field. The MS03 model is deposited on Model Archive (http://www.modelarchive.org/project/index/doi/ma-am3yh) and can be downloaded from there. All three of these files can be downloaded from our website (http://spudlab.stanford.edu/homology-models/). The three models are very similar to one another and are all consistent with the conclusions and considerations discussed in our 2016 and 2017 publications (Adhikari et al. 2016; Kawana et al. 2017; Nag et al. 2017). All three models received a good evaluation score by the evaluation metrics TSVMod scores, Z-score (DOPE) and GA341 scores as described at https://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/modbase.html. All figures in this review are based on MS03.
When we highlighted the residues of the myosin mesa on our human β-cardiac myosin IHM models, we were amazed to see that the mesas of the blocked and free heads (pink, Fig. 7a) cradle the proximal S2. This observation initiated a series of studies on the physiological regulation of Na as well as the role of Na in the hyper-contractility seen clinically by HCM mutations in both myosin and MyBP-C (Adhikari et al. 2016; Kawana et al. 2017; Nag et al. 2017). Thus, to test the notion that HCM-causing mutations increase Na, releasing myosin heads into the actively contracting pool in the absence of adrenergic stimulation, thus leading to the hyper-contractility seen clinically, we examined four HCM-causing mutations that lie in the zone where proximal S2 is thought to bind according to the working model (zone 2; Fig. 7a). Strikingly, each of these four HCM mutations, namely R249Q, H251N and R453C on the mesa and D906G on proximal S2, significantly weakened the affinity of proximal S2 for sS1, the truncated form of S1 missing the RLC, while the three HCM mutations lying outside of zone 2, i.e. R403Q, D239N and R870H, did not (Adhikari et al. 2016; Nag et al. 2017). If the structural model and the overall hypothesis on the effects of HCM mutations on increasing Na are correct, then there should be good correspondence between the localization of HCM mutations and the stability of the various proposed domain interaction sites, and thus far this has proven true. One should, however, design experiments to disprove one’s hypotheses, and there is much experimentation that needs to be done regarding the working hypotheses discussed here.
What other interaction sites are involved in the folded-back IHM off-state? Apart from the interaction between the blocked-head S1 mesa and the proximal S2 tail of myosin, the two heads of the myosin also interact directly with each other (Fig. 7b). These interactions were first reported in chicken smooth muscle HMM 2D crystals (Liu et al. 2003; Wendt et al. 1999, 2001), and then observed in 3D reconstructions from electron micrographs in intact tarantula thick filaments (Woodhead et al. 2005) and analyzed by Alamo et al. (2008, 2016). The interaction between the two heads is mediated by a blocked-head surface, which is adjacent to both the mesa and the actin-binding surfaces, binding to the converter domain of the free head (Figs. 4, 7, bright green residues). Strikingly, most of the known converter HCM mutations lie at this interface, and we hypothesized that they may weaken the head–head interaction, thereby shifting the equilibrium between the closed IHM ‘off state’ and the open ‘on state’ in the direction of increased Na (Kawana et al. 2017; Nag et al. 2017). Appropriate assays need to be developed to specifically probe this S1–S1 interaction, and this is another high priority in the field.
In the sarcomere, other molecular interactions, such as RLC–RLC (Alamo et al. 2008, 2016; Brito et al. 2011; Nogara et al. 2016b), intermolecular head–head (Alamo et al. 2008, 2016; Woodhead et al. 2005), and head–LMM (Alamo et al. 2016; Woodhead et al. 2005) interactions, have been described. In addition, S2–LMM and head–titin interactions may contribute to the IHM state. The interactions of myosin with MyBP-C seem likely to be central to increases in Na by HCM mutations. The C1–C2 interaction with proximal S2 studied by Gruen et al. (Gruen and Gautel 1999) is weakened or abrogated in the disease-causing mutations R870H and E924K. Again, specific assays for focusing on these domain interactions and the effect of local HCM mutations on their affinities need to be established.
The importance of dividing our homology-modeled structure into three zones (Fig. 7a) is to appreciate the location of the HCM-causing mutations at the interfaces of the putative important interactions and to provide a working hypothesis for future experiments. Thus zone 3 mesa mutations, which include R403Q, may weaken the C0–C2-free head interaction (Figs. 6b, 7a), while zone 1 mesa mutations, which include R663H, may weaken the C5–C6-blocked head interactions (Figs. 6c, 7a). Future experiments should be directed at testing such predictions. R403Q is unique in that it lies at the junction of the mesa, the blocked head domain that interacts with the converter surface of the free head (the S1–S1 interaction region), and the actin-binding site for myosin (Fig. 4b). Thus, R403Q could be weakening interactions in zone 3, the S1–S1 interaction region and/or the actin-binding interface.
We emphasize that our models serve the purpose of working hypotheses and should not be viewed as actual structures. As working hypotheses, they can guide experiments to test the models. Another word of caution—we do not consider any of the current IHM models from EM reconstructions to be of sufficient resolution to embark on analyses of the effects of HCM residue changes on neighboring interacting residues, rates of ADP or release of inorganic phosphate, changes in switch 1 and 2, binding affinities between the head and S2 or interactions with MyBP-C, and so forth. Furthermore, the actual human β-cardiac folded IHM structure would be expected to be different in high-resolution detail from, for example, the tarantula skeletal myosin IHM structure, given that more than 40% of residues differ between the S1 heads of these two myosins. High-resolution EM reconstruction images and/or X-ray crystallography structures using purified human β-cardiac myosin or HMM will be necessary to begin to assess the more detailed aspects of the effects of the HCM mutations, and this should be a high priority effort in the field. Nonetheless, our working models of human β-cardiac myosin IHM structures serve as a reasonable guide for experiments to test them. This is one area that is just in its infancy, and a huge amount of work lies ahead.
Small molecule effectors are promising agents for future treatment of HCM
Conclusion and perspectives
Over the past five decades our understanding of muscle physiology, biochemistry and biophysics has greatly advanced. We have a good understanding of how various sarcomeric proteins function and their roles in muscle contractility. Now focus is shifting to understanding the molecular basis of disease states of these proteins. Although a significant knowledge of myosin biomechanics is at hand, there is much to be learned to understand how HCM mutations alter the system at the molecular level to cause the hyper-contractility seen clinically. This is an exciting area of research that deserves a great deal of experimental effort, a perfect area for many new investigators to become involved in. There is much to be done.
We thank M.S. Sunitha for extensive help in homology modeling of the human β-cardiac S1, the IHM state of the human β-cardiac myosin, and the full length MyBP-C.
D.V.T., A.S.A., S.S.S., K.M.R., and J.A.S. wrote and edited the manuscript. Molecular modeling studies were performed by J.A.S., and figures were prepared by D.V.T. and J.A.S.
Compliance with ethical standards
This work was funded by NIH grants GM33289 and HL117138 to J.A.S., a Stanford Lucile Packard CHRI Postdoctoral Award (UL1 TR001085) and American Heart Association Postdoctoral Fellowship (17POST33411070) to D.V.T., and a Stanford Lucile Packard CHRI Postdoctoral Award (UL1 TR001085), Stanford ChEM-H Postdocs at the Interface Award, and American Heart Association Postdoctoral Fellowship (16POST30890005) to ASA.
Conflict of interest
J.A.S. is a founder of Cytokinetics and MyoKardia and a member of their scientific advisory boards. K.M.R. is on the SAB of MyoKardia.
This manuscript does not contain any work involving human subjects or animal models.
- Ababou A, Rostkova E, Mistry S, Le Masurier C, Gautel M, Pfuhl M (2008) Myosin binding protein C positioned to play a key role in regulation of muscle contraction: structure and interactions of domain C1. J Mol Biol 384:615–630. doi: 10.1016/j.jmb.2008.09.065 PubMedPubMedCentralCrossRefGoogle Scholar
- Adhikari AS, Kooiker KB, Sarkar SS, Liu C, Bernstein D, Spudich JA, Ruppel KM (2016) Early-onset hypertrophic cardiomyopathy mutations significantly increase the velocity, force, and actin-activated ATPase activity of human beta-cardiac myosin. Cell Rep 17:2857–2864. doi: 10.1016/j.celrep.2016.11.040 PubMedCrossRefGoogle Scholar
- Ait-Mou Y, Hsu K, Farman GP, Kumar M, Greaser ML, Irving TC, de Tombe PP (2016) Titin strain contributes to the frank-Starling law of the heart by structural rearrangements of both thin- and thick-filament proteins. Proc Natl Acad Sci USA 113:2306–2311. doi: 10.1073/pnas.1516732113 PubMedPubMedCentralCrossRefGoogle Scholar
- Alamo L, Li XE, Espinoza-Fonseca LM, Pinto A, Thomas DD, Lehman W, Padron R (2015) Tarantula myosin free head regulatory light chain phosphorylation stiffens N-terminal extension, releasing it and blocking its docking back. Mol BioSyst 11:2180–2189. doi: 10.1039/c5mb00163c PubMedPubMedCentralCrossRefGoogle Scholar
- Alamo L, Qi D, Wriggers W, Pinto A, Zhu J, Bilbao A, Gillilan RE, Hu S, Padrón R (2016) Conserved intramolecular interactions maintain myosin interacting-heads motifs explaining tarantula muscle super-relaxed state structural basis. J Mol Biol 428(6):1142–1164. doi: 10.1016/j.jmb.2016.01.027
- Alamo L, Wriggers W, Pinto A, Bartoli F, Salazar L, Zhao FQ, Craig R, Padron R (2008) Three-dimensional reconstruction of tarantula myosin filaments suggests how phosphorylation may regulate myosin activity. J Mol Biol 384:780–797. doi: 10.1016/j.jmb.2008.10.013
- Bardswell SC, Cuello F, Rowland AJ, Sadayappan S, Robbins J, Gautel M, Walker JW, Kentish JC, Avkiran M (2010) Distinct sarcomeric substrates are responsible for protein kinase D-mediated regulation of cardiac myofilament Ca2+ sensitivity and cross-bridge cycling. J Biol Chem 285:5674–5682. doi: 10.1074/jbc.M109.066456
- Blankenfeldt W, Thoma NH, Wray JS, Gautel M, Schlichting I (2006) Crystal structures of human cardiac beta-myosin II S2-Delta provide insight into the functional role of the S2 subfragment. Proc Natl Acad Sci USA 103:17713–17717. doi: 10.1073/pnas.0606741103 PubMedPubMedCentralCrossRefGoogle Scholar
- Brito R, Alamo L, Lundberg U, Guerrero JR, Pinto A, Sulbaran G, Gawinowicz MA, Craig R, Padron R (2011) A molecular model of phosphorylation-based activation and potentiation of tarantula muscle thick filaments. J Mol Biol 414:44–61. doi: 10.1016/j.jmb.2011.09.017
- Carrier L, Knoll R, Vignier N, Keller DI, Bausero P, Prudhon B, Isnard R, Ambroisine ML, Fiszman M, Ross J, Jr., Schwartz K, Chien KR (2004) Asymmetric septal hypertrophy in heterozygous cMyBP-C null mice. Cardiovasc Res 63:293–304. doi: 10.1016/j.cardiores.2004.04.009
- Cazorla O, Szilagyi S, Vignier N, Salazar G, Kramer E, Vassort G, Carrier L, Lacampagne A (2006) Length and protein kinase A modulations of myocytes in cardiac myosin binding protein C-deficient mice. Cardiovasc Res 69:370–380. doi: 10.1016/j.cardiores.2005.11.009
- Colson BA, Thompson AR, Espinoza-Fonseca LM, Thomas DD (2016) Site-directed spectroscopy of cardiac myosin-binding protein C reveals effects of phosphorylation on protein structural dynamics. Proc Natl Acad Sci USA 113:3233–3238. doi: 10.1073/pnas.1521281113 PubMedPubMedCentralCrossRefGoogle Scholar
- Craig R, Lee KH, Mun JY, Torre I, Luther PK (2014) Structure, sarcomeric organization, and thin filament binding of cardiac myosin-binding protein-C. Pflugers Arch 466:425–431. doi: 10.1007/s00424-013-1426-6
- Dirkx E, Cazorla O, Schwenk RW, Lorenzen-Schmidt I, Sadayappan S, Van Lint J, Carrier L, van Eys GJ, Glatz JF, Luiken JJ (2012) Protein kinase D increases maximal Ca2+−activated tension of cardiomyocyte contraction by phosphorylation of cMyBP-C-Ser315. Am J Physiol Heart Circ Physiol 303:H323–H331. doi: 10.1152/ajpheart.00749.2011
- Green EM, Wakimoto H, Anderson RL, Evanchik MJ, Gorham JM, Harrison BC, Henze M, Kawas R, Oslob JD, Rodriguez HM, Song Y, Wan W, Leinwand LA, Spudich JA, McDowell RS, Seidman JG, Seidman CE (2016) A small-molecule inhibitor of sarcomere contractility suppresses hypertrophic cardiomyopathy in mice. Science 351:617–621Google Scholar
- Harris SP, Bartley CR, Hacker TA, McDonald KS, Douglas PS, Greaser ML, Powers PA, Moss RL (2002) Hypertrophic cardiomyopathy in cardiac myosin binding protein-C knockout mice. Circ Res 90:594–601Google Scholar
- Herman DS , Lam L, Taylor MR, Wang L, Teekakirikul P, Christodoulou D, Conner L, DePalma SR, McDonough B, Sparks E, Teodorescu DL, Cirino AL, Banner NR, Pennell DJ, Graw S, Merlo M, Di Lenarda A, Sinagra G, Bos JM, Ackerman MJ, Mitchell RN, Murry CE, Lakdawala NK, Ho CY, Barton PJ, Cook SA, Mestroni L, Seidman JG, Seidman CE (2012) Truncations of titin causing dilated cardiomyopathy. N Engl J Med 366:619–628. doi: 10.1056/NEJMoa1110186
- Homburger JR, Green EM, Caleshu C, Sunitha MS, Taylor RE, Ruppel KM, Metpally RP, Colan SD, Michels M, Day SM, Olivotto I, Bustamante CD, Dewey FE, Ho CY, Spudich JA, Ashley EA (2016) Multidimensional structure-function relationships in human beta-cardiac myosin from population-scale genetic variation. Proc Natl Acad Sci USA 113:6701–6706. doi: 10.1073/pnas.1606950113
- Howard J (2001) Mechanics of motor proteins and the cytoskeleton. Sinauer, SunderlandGoogle Scholar
- Jia W, Shaffer JF, Harris SP, Leary JA (2010) Identification of novel protein kinase A phosphorylation sites in the M-domain of human and murine cardiac myosin binding protein-C using mass spectrometry analysis. J Proteome Res 9:1843–1853. doi: 10.1021/pr901006h PubMedPubMedCentralCrossRefGoogle Scholar
- Jung HS, Billington N, Thirumurugan K, Salzameda B, Cremo CR, Chalovich JM, Chantler PD, Knight PJ (2011) Role of the tail in the regulated state of myosin 2. J Mol Biol 408:863–878. doi: 10.1016/j.jmb.2011.03.019
- Jung HS, Burgess SA, Billington N, Colegrave M, Patel H, Chalovich JM, Chantler PD, Knight PJ (2008a) Conservation of the regulated structure of folded myosin 2 in species separated by at least 600 million years of independent evolution. Proc Natl Acad Sci USA 105:6022–6026. doi: 10.1073/pnas.0707846105
- Krieger E, Joo K, Lee J, Lee J, Raman S, Thompson J, Tyka M, Baker D, Karplus K (2009) Improving physical realism, stereochemistry, and side-chain accuracy in homology modeling: four approaches that performed well in CASP8. Proteins 77[Suppl 9]:114–122. doi: 10.1002/prot.22570
- Linari M, Brunello E, Reconditi M, Fusi L, Caremani M, Narayanan T, Piazzesi G, Lombardi V, Irving M (2015) Force generation by skeletal muscle is controlled by mechanosensing in myosin filaments. Nature 528:276–279. doi: 10.1038/nature15727
- Lowey S, Lesko LM, Rovner AS, Hodges AR, White SL, Low RB, Rincon M, Gulick J, Robbins J (2008) Functional effects of the hypertrophic cardiomyopathy R403Q mutation are different in an alpha- or beta-myosin heavy chain backbone. J Biol Chem 283:20579–20589. doi: 10.1074/jbc.M800554200
- Luther PK, Bennett PM, Knupp C, Craig R, Padron R, Harris SP, Patel J, Moss RL (2008) Understanding the organisation and role of myosin binding protein C in normal striated muscle by comparison with MyBP-C knockout cardiac muscle. J Mol Biol 384:60–72. doi: 10.1016/j.jmb.2008.09.013
- Luther PK, Winkler H, Taylor K, Zoghbi ME, Craig R, Padron R, Squire JM, Liu J (2011) Direct visualization of myosin-binding protein C bridging myosin and actin filaments in intact muscle. Proc Natl Acad Sci USA 108:11423–11428. doi: 10.1073/pnas.1103216108
- Maron BJ (2010) Hypertrophic cardiomyopathy. In: Bonow RO, Mann DL, Zipes DP, Libby P (eds) Braunwald’s heart disease: a textbook of cardiovascular medicine. Elsevier, Amsterdam, pp 1582–1594Google Scholar
- Maron BJ, Gardin JM, Flack JM, Gidding SS, Kurosaki TT, Bild DE (1995) Prevalence of hypertrophic cardiomyopathy in a general population of young adults: echocardiographic analysis of 4111 subjects in the CARDIA study. Circulation 92:785–789. doi: 10.1161/01.cir.92.4.785 PubMedCrossRefGoogle Scholar
- Matsumoto Y, Hayashi T, Inagaki N, Takahashi M, Hiroi S, Nakamura T, Arimura T, Nakamura K, Ashizawa N, Yasunami M, Ohe T, Yano K, Kimura A (2005) Functional analysis of titin/connectin N2-B mutations found in cardiomyopathy. J Muscle Res Cell Motil 26:367–374. doi: 10.1007/s10974-005-9018-5
- McNamara JW, Li A, Smith NJ, Lal S, Graham RM, Kooiker KB, van Dijk SJ, Remedios CG, Harris SP, Cooke R (2016) Ablation of cardiac myosin binding protein-C disrupts the super-relaxed state of myosin in murine cardiomyocytes. J Mol Cell Cardiol 94:65–71. doi: 10.1016/j.yjmcc.2016.03.009
- Moore JR, Campbell SG, Lehman W (2016) Structural determinants of muscle thin filament cooperativity. Arch Biochem Biophys 594:8–17. doi: 10.1016/j.abb.2016.02.016
- Moore JR, Leinwand L, Warshaw DM (2012) Understanding cardiomyopathy phenotypes based on the functional impact of mutations in the myosin motor. Circ Res 111:375–385. doi: 10.1161/circresaha.110.223842
- Mun JY, Previs MJ, Yu HY, Gulick J, Tobacman LS, Beck Previs S, Robbins J, Warshaw DM, Craig R (2014) Myosin-binding protein C displaces tropomyosin to activate cardiac thin filaments and governs their speed by an independent mechanism. Proc Natl Acad Sci USA 111:2170–2175. doi: 10.1073/pnas.1316001111
- Nag S, Sommese RF, Ujfalusi Z, Combs A, Langer S, Sutton S, Leinwand LA, Geeves MA, Ruppel KM, Spudich JA (2015) Contractility parameters of human beta-cardiac myosin with the hypertrophic cardiomyopathy mutation R403Q show loss of motor function. Sci Adv 1:e1500511. doi: 10.1126/sciadv.1500511
- Nag S, Trivedi DV, Sarkar SS, Adhikari AS, Sunitha MS, Sutton S, Ruppel KM, Spudich JA (2017) The myosin mesa and the basis of hypercontractility caused by hypertrophic cardiomyopathy mutations. Nat Struct Mol Biol 24(6):525–533. doi: 10.1038/nsmb.3408
- Nogara L, Naber N, Pate E, Canton M, Reggiani C, Cooke R (2016a) Piperine’s mitigation of obesity and diabetes can be explained by its up-regulation of the metabolic rate of resting muscle. Proc Natl Acad Sci USA 113:13009–13014. doi: 10.1073/pnas.1607536113 PubMedPubMedCentralCrossRefGoogle Scholar
- Obermann WM, Gautel M, Weber K, Furst DO (1997) Molecular structure of the sarcomeric M band: mapping of titin and myosin binding domains in myomesin and the identification of a potential regulatory phosphorylation site in myomesin. EMBO J 16:211–220. doi: 10.1093/emboj/16.2.211 PubMedPubMedCentralCrossRefGoogle Scholar
- Palmiter KA, Tyska MJ, Haeberle JR, Alpert NR, Fananapazir L, Warshaw DM (2000) R403Q and L908V mutant beta-cardiac myosin from patients with familial hypertrophic cardiomyopathy exhibit enhanced mechanical performance at the single molecule level. J Muscle Res Cell Motil 21:609–620PubMedCrossRefGoogle Scholar
- Previs MJ, Mun JY, Michalek AJ, Previs SB, Gulick J, Robbins J, Warshaw DM, Craig R (2016) Phosphorylation and calcium antagonistically tune myosin-binding protein C's structure and function Proc Natl Acad Sci USA. 113:3239–3244 doi: 10.1073/pnas.1522236113
- Previs MJ, Prosser BL, Mun JY, Previs SB, Gulick J, Lee K, Robbins J, Craig R, Lederer WJ, Warshaw DM (2015) Myosin-binding protein C corrects an intrinsic inhomogeneity in cardiac excitation-contraction coupling Sci Adv 1. doi: 10.1126/sciadv.1400205
- Reconditi M, Caremani M, Pinzauti F, Powers JD, Narayanan T, Stienen GJ, Linari M, Lombardi V, Piazzesi G (2017) Myosin filament activation in the heart is tuned to the mechanical task. Proc Natl Acad Sci USA 114:3240–3245. doi: 10.1073/pnas.1619484114
- Sadayappan S, Gulick J, Osinska H, Barefield D, Cuello F, Avkiran M, Lasko VM, Lorenz JN, Maillet M, Martin JL, Brown JH, Bers DM, Molkentin JD, James J, Robbins J (2011) A critical function for Ser-282 in cardiac myosin binding protein-C phosphorylation and cardiac function. Circ Res 109:141–150. doi: 10.1161/CIRCRESAHA.111.242560
- Seidman CE, Seidman JG (2000) Hypertrophic cardiomyopathy. In: Scriver CR, Beaudet AL, Valle D, Sly WS, Childs KW, Vogelstein B (eds) The metabolic and molecular bases of inherited disease. McGraw-Hill, New York, pp 5532–5452Google Scholar
- Sommese RF, Sung J, Nag S, Sutton S, Deacon JC, Choe E, Leinwand LA, Ruppel K, Spudich JA (2013) Molecular consequences of the R453C hypertrophic cardiomyopathy mutation on human beta-cardiac myosin motor function. Proc Natl Acad Sci USA 110:12607–12612. doi: 10.1073/pnas.1309493110
- Spudich JA, Aksel T, Bartholomew SR, Nag S, Kawana M, Yu EC, Sarkar SS, Sung J, Sommese RF, Sutton S, Cho C, Adhikari AS, Taylor R, Liu C, Trivedi D, Ruppel KM (2016) Effects of hypertrophic and dilated cardiomyopathy mutations on power output by human beta-cardiac myosin. J Exp Biol 219:161–167. doi: 10.1242/jeb.125930
- Sulbaran G, Alamo L, Pinto A, Marquez G, Mendez F, Padron R, Craig R (2015) An invertebrate smooth muscle with striated muscle myosin filaments. Proc Natl Acad Sci USA 112:E5660–E5668. doi: 10.1073/pnas.1513439112
- Sulbaran G, Biasutto A, Alamo L, Riggs C, Pinto A, Mendez F, Craig R, Padron R (2013) Different head environments in tarantula thick filaments support a cooperative activation process. Biophys J 105:2114–2122. doi: 10.1016/j.bpj.2013.09.001
- Syamaladevi DP, Sunitha MS, Kalaimathy S, Reddy CC, Iftekhar M, Pasha SN, Sowdhamini R (2012) Myosinome: a database of myosins from select eukaryotic genomes to facilitate analysis of sequence-structure-function relationships. Bioinform Biol Insights 6:247–254. doi: 10.4137/BBI.S9902 PubMedPubMedCentralGoogle Scholar
- Toepfer C, Caorsi V, Kampourakis T, Sikkel MB, West TG, Leung MC, Al-Saud SA, MacLeod KT, Lyon AR, Marston SB, Sellers JR, Ferenczi MA (2013) Myosin regulatory light chain (RLC) phosphorylation change as a modulator of cardiac muscle contraction in disease. J Biol Chem 288:13446–13454. doi: 10.1074/jbc.M113.455444
- Toyoshima YY, Kron SJ, McNally EM, Niebling KR, Toyoshima C, Spudich JA (1987) Myosin subfragment-1 is sufficient to move actin filaments in vitro Nature 328:536–539. doi: 10.1038/328536a0
- Waldmuller S, Erdmann J, Binner P, Gelbrich G, Pankuweit S, Geier C, Timmermann B, Haremza J, Perrot A, Scheer S, Wachter R, Schulze-Waltrup N, Dermintzoglou A, Schonberger J, Zeh W, Jurmann B, Brodherr T, Borgel J, Farr M, Milting H, Blankenfeldt W, Reinhardt R, Ozcelik C, Osterziel KJ, Loeffler M, Maisch B, Regitz-Zagrosek V, Schunkert H, Scheffold T, German Competence Network Heart F (2011) Novel correlations between the genotype and the phenotype of hypertrophic and dilated cardiomyopathy: results from the German competence network heart failure. Eur J Heart Fail 13:1185–1192. doi: 10.1093/eurjhf/hfr074
- Weith A, Sadayappan S, Gulick J, Previs MJ, Vanburen P, Robbins J, Warshaw DM (2012) Unique single molecule binding of cardiac myosin binding protein-C to actin and phosphorylation-dependent inhibition of actomyosin motility requires 17 amino acids of the motif domain. J Mol Cell Cardiol 52:219–227. doi: 10.1016/j.yjmcc.2011.09.019 PubMedCrossRefGoogle Scholar
- Wendt T, Taylor D, Trybus KM, Taylor K (2001) Three-dimensional image reconstruction of dephosphorylated smooth muscle heavy meromyosin reveals asymmetry in the interaction between myosin heads and placement of subfragment 2. Proc Natl Acad Sci USA 98:4361–4366. doi: 10.1073/pnas.071051098 PubMedPubMedCentralCrossRefGoogle Scholar
- Yang Y, Gourinath S, Kovacs M, Nyitray L, Reutzel R, Himmel DM, O'Neall-Hennessey E, Reshetnikova L, Szent-Gyorgyi AG, Brown JH, Cohen C (2007) Rigor-like structures from muscle myosins reveal key mechanical elements in the transduction pathways of this allosteric motor. Structure 15:553–564. doi: 10.1016/j.str.2007.03.010
- Zhang X, Kampourakis T, Yan Z, Sevrieva I, Irving M, Sun YB (2017) Distinct contributions of the thin and thick filaments to length-dependent activation in heart muscle. Elife 6. doi: 10.7554/eLife.24081
- Zoghbi ME, Woodhead JL, Moss RL, Craig R (2008) Three-dimensional structure of vertebrate cardiac muscle myosin filaments. Proc Natl Acad Sci USA 105:2386–2390. doi: 10.1073/pnas.0708912105
- Zot AS, Potter JD (1987) Structural aspects of troponin-tropomyosin regulation of skeletal muscle contraction. Annu Rev Biophys Biophys Chem 16:535–559. doi: 10.1146/annurev.bb.16.060187.002535
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