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Biophysical Reviews

, 1:161 | Cite as

Single-molecule Förster resonance energy transfer studies of RNA structure, dynamics and function

  • Mark HelmEmail author
  • Andrei Yu Kobitski
  • G. Ulrich Nienhaus
Review

Abstract

Single-molecule fluorescence microscopy experiments on RNA molecules brought to light the highly complex dynamics of key biological processes, including RNA folding, catalysis of ribozymes, ligand sensing of riboswitches and aptamers, and protein synthesis in the ribosome. By using highly advanced biophysical spectroscopy techniques in combination with sophisticated biochemical synthesis approaches, molecular dynamics of individual RNA molecules can be observed in real time and under physiological conditions in unprecedented detail that cannot be achieved with bulk experiments. Here, we review recent advances in RNA folding and functional studies of RNA and RNA-protein complexes addressed by using single-molecule Förster (fluorescence) resonance energy transfer (smFRET) technique.

Keywords

RNA RNA folding RNA-protein complexes Single-molecule Förster resonance energy transfer technique 

Notes

Acknowledgements

The authors acknowledge funding by the Volkswagen Foundation and the Deutsche Forschungsgemeinschaft (DFG). We thank Martin Hengesbach for technical assistance.

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Copyright information

© International Union for Pure and Applied Biophysics (IUPAB) and Springer 2009

Authors and Affiliations

  • Mark Helm
    • 1
    • 2
    Email author
  • Andrei Yu Kobitski
    • 3
  • G. Ulrich Nienhaus
    • 3
    • 4
  1. 1.Institute of PharmacyUniversity of MainzMainzGermany
  2. 2.Institute of Pharmacy and Molecular BiotechnologyHeidelberg UniversityHeidelbergGermany
  3. 3.Institute of Applied Physics and Center for Functional Nanostructures (CFN)Karlsruhe Institute of Technology (KIT)KarlsruheGermany
  4. 4.Department of PhysicsUniversity of Illinois at Urbana-ChampaignUrbanaUSA

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