Biophysical Reviews

, Volume 1, Issue 2, pp 95–103 | Cite as

Vibrational spectroscopic mapping and imaging of tissues and cells

  • Elizabeth A. Carter
  • Koman K. Tam
  • Robert S. Armstrong
  • Peter A. Lay


Vibrational spectroscopic mapping (point-by-point measurement) and imaging of biological samples (cells and tissues) covering Fourier-transform infrared (FTIR) and Raman spectroscopies has opened up many exciting new avenues to explore biochemical architecture and processes within healthy and diseased cells and tissues, including medical diagnostics and drug design.


Biological tissues Cells Imaging Mapping Vibrational spectroscopy 



The breast cancer research was supported by a grant from The University of Sydney, Cancer Research Fund, the Australian Synchrotron Research Program, which is funded by the Commonwealth of Australia under the Major National Research Facilities Program for research conducted at NSSRC, and the Australian Synchrotron. The authors also thank Dr. Brian Reedy for the use of the FTIR imaging instrument at the University of Technology, Sydney. We are grateful to the ARC for funding of some of the research reported herein through RIEF and LIEF grants and ARC Discovery grants to PAL, including Australian Professorial Fellowships. We also thank Carolyn Mountford from the Institute for Magnetic Resonance Research, The University of Sydney, and Peter Russell from the Department of Anatomical Pathology, Royal Prince Alfred Hospital, for provision of the breast cancer samples used to obtain the spectra in Figs. 1, 2 and 4.


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Copyright information

© International Union for Pure and Applied Biophysics (IUPAB) and Springer 2009

Authors and Affiliations

  • Elizabeth A. Carter
    • 1
  • Koman K. Tam
    • 1
    • 2
  • Robert S. Armstrong
    • 1
  • Peter A. Lay
    • 1
  1. 1.Vibrational Spectroscopy Facility, School of ChemistryThe University of SydneySydneyAustralia
  2. 2.Analytical Technologies Division–Biolab (Aust) Pty LtdVictoriaAustralia

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