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Mycotoxin Research

, Volume 34, Issue 3, pp 223–227 | Cite as

Occurrence of ochratoxin A in Astragalus propinquus root and its transfer to decoction

  • Jakub Toman
  • Vladimir Ostry
  • Yann Grosse
  • Tomas Roubal
  • Frantisek Malir
Original Article
  • 36 Downloads

Abstract

The aim of this study was to conduct a survey assessing (a) the ochratoxin A (OTA) content in different samples of Astragalus propinquus root (AR), one of the fundamental herbs in traditional Chinese medicine, and (b) the rate of OTA transfer to AR decoctions that are traditionally used to reduce general weakness and increase overall vitality. A validated method of high-performance liquid chromatography with fluorescence detection (HPLC-FLD) was used to determine OTA concentrations in AR samples and AR decoctions. The limit of quantification was 0.35 ng/g; the recovery of the HPLC method for AR samples was 82%; and the relative standard deviation (SD) of repeatability was 2.6%. All 40 tested AR samples were positive, with a mean value of 451.0 ng/g (range, 28.8–1700.0 ng/g). The transfer rate of OTA to decoctions, from a naturally contaminated and homogenized AR sample (internal reference material) with a concentration of OTA of 288.9 ng/g ± 12.3 (SD), was 83.4% ± 8.5 (SD). We believe it is necessary to continue OTA monitoring in AR and other herbal products, estimate the actual human usual intake, and perform health risk assessment.

Keywords

Astragalus propinquus Schischkin Herbal food supplement Herbal products HPLC-FLD Ochratoxin A Traditional Chinese medicine 

Notes

Funding information

This study received a financial support from the specific research project (No. 2113/2016) of Faculty of Science, University Hradec Kralove, Czech Republic, and from the project of Ministry of Health, Czech Republic conceptual development of research organization (“National Institute of Public Health – NIPH, IN 75010330”).

Compliance with ethical standards

Conflict of interest

The authors confirm that there are no known conflicts of interest associated with this publication.

References

  1. Aldarmaa J, Liu Z, Long J, Mo X, Ma J, Liu J (2010) Anti-convulsant effect and mechanism of Astragalus mongholicus extract in vitro and in vivo: protection against oxidative damage and mitochondrial dysfunction. Neurochem Res 35(1):33–41.  https://doi.org/10.1007/s11064-009-0027-4 CrossRefPubMedGoogle Scholar
  2. Bedir E, Pugh N, Calis I, Pasco DS, Khan IA (2000) Immunostimulatory effects of cycloartane-type triterpene glycosides from astragalus species. Biol Pharm Bull 23(7):834–837CrossRefPubMedGoogle Scholar
  3. Bhat R, Sridhar KR, Karim AA (2010) Mycotoxins in food and feed: present status and future concerns. Compr Rev Food Sci Food Saf 9(1):57–81.  https://doi.org/10.1111/j.1541-4337.2009.00094.x CrossRefGoogle Scholar
  4. Bone K., Mills S. (2013) Principles and practice of phytotherapy (2nd ed.). Edinborough: Churchill Livingstone/Elsevier. 381–389. doi: http://dx.doi.Org/10.1016/B978-0-443-06992-5.00001-3
  5. Bullerman LB, Bianchini A (2007) Stability of mycotoxins during food processing. Int J Food Microbiol 119(1–2):140–146.  https://doi.org/10.1016/j.ijfoodmicro.2007.07.035 CrossRefPubMedGoogle Scholar
  6. Chan JYW, Lam FC, Leung PC, Che CT, Fung KP (2009) Antihyperglycemic and antioxidative effects of a herbal formulation of Radix Astragali, Radix Codonopsis and Cortex Lycii in a mouse model of type 2 diabetes mellitus. Phytother Res 23(5):658–665.  https://doi.org/10.1002/ptr.2694 CrossRefPubMedGoogle Scholar
  7. Chang YX, Sun YG, Li J, Zhang QH, Guo XR, Zhang BL, Jin H, Gao XM (2012) The experimental study of Astragalus membranaceus on meridian tropsim: the distribution study of astragaloside IV in rat tissues. J Chromatogr B Analyt Technol Biomed Life Sci 911:71–75.  https://doi.org/10.1016/j.jchromb.2012.10.024 CrossRefPubMedGoogle Scholar
  8. Chen A, Sun L, Yuan H, Wu A, Lu J, Ma S (2017) A holistic strategy for quality and safety control of traditional Chinese medicines by the “iVarious” standard system. J Pharm Anal 7(5):271–279.  https://doi.org/10.1016/j.jpha.2017.07.008 CrossRefPubMedPubMedCentralGoogle Scholar
  9. Chinese Pharmacopoeia Commission (2010) Pharmacopoeia of the People’s Republic of China 2010. Set of 3, Chinese Edition, China Medical Science and Technology Press, BeijingGoogle Scholar
  10. Cho WCS, Leung KN (2007) In vitro and in vivo anti-tumor effects of Astragallus membranaceus. Cancer Lett 252:43–54.  https://doi.org/10.1016/j.canlet.2006.12.001 CrossRefPubMedGoogle Scholar
  11. Choi HS, Joo SJ, Yoon HS, Kim KS, Song IG, Min KB (2007) Quality characteristic of hwangki (Astragalus membranaceus) chungkukjang during fermentation. Korean Journal of Food Preservation 14(4):356–363Google Scholar
  12. Creppy EE, Castegnaro M, Grosse Y, Meriaux J, Manier C, Moncharmont P, Waller C (1993) Etude de l’ochratoxicose humaine dans trois regions de France. Alsace, Aquitaine et Region Rhone-Alpes. In: Creppy EE, Castegnaro M, Dirheimer G (eds) Human Ochratoxicosis and Its Pathologies. John Libbey Eurotext, Montrouge, 1993, pp 147–158Google Scholar
  13. Dohnal V, Dvorak V, Malir F, Ostry V, Roubal T (2013) A comparison of ELISA and HPLC methods for determination of ochratoxin A in human blood serum in the Czech Republic. Food Chem Toxicol 62:427–431.  https://doi.org/10.1016/j.fct.2013.09.010 CrossRefPubMedGoogle Scholar
  14. EFSA (2006) European Food Safety Authority. Opinion of the scientific panel on contaminants in the food chain on a request from the commission related to ochratoxin A in food. EFSA J 365:1–56.  https://doi.org/10.2903/j.efsa.2006.365 Google Scholar
  15. EU (2006) Commission Regulation (EC) No 1881/2006 of 19 December 2006 setting maximum levels for certain contaminants in foodstuffs (Text with EEA relevance). Off J Eur Union L364:5–24Google Scholar
  16. Fu J, Wang Z, Huang L, Zheng S, Wang D, Chen S, Zhang H, Yang S (2014) Review of the botanical characteristics, phytochemistry, and pharmacology of Astragalus membranaceus (Huangqi). Phytother Res 28(9):1275–1283.  https://doi.org/10.1002/ptr.5188 CrossRefPubMedGoogle Scholar
  17. Halt M (1998) Moulds and mycotoxins in herb tea and medicinal plants. Eur J Epidemiol 14(3):269–274CrossRefPubMedGoogle Scholar
  18. Heussner AH, Bingle LEH (2015) Comparative Ochratoxin toxicity: a review of the available data. Toxins 7:4253–4282.  https://doi.org/10.3390/toxins7104253 CrossRefPubMedPubMedCentralGoogle Scholar
  19. Huang Y, Tang G, Zhang T, Fillet M, Crommen J, Jiang Z (2018) Supercritical fluid in traditional Chinese medicine analysis. J Pharm Biomed Anal 147:65–80.  https://doi.org/10.1016/j.jpba.2017.08.021 CrossRefPubMedGoogle Scholar
  20. IARC (1993) Monographs on the evaluation of carcinogenic risks to humans: some naturally occuring substances: food items and constituents, heterocyclic aromatic amines and mycotoxins. Vol. 56. France: IARC, Lyon, pp 489–524Google Scholar
  21. Ionkova I, Shkondrov A, Krasteva I, Ionkov T (2014) Recent progress in phytochemistry: pharmacology and biotechnology of Astragalus saponins. Phytochem Rev 13:343–374  https://doi.org/10.1007/s11101-014-9347-3 CrossRefGoogle Scholar
  22. Jiang Y, David B, Tu PF, Barbin Y (2010) Recent analytical approaches in quality control of traditional Chinese medicines—a review. AnalChimActa 657:9–18.  https://doi.org/10.1016/j.aca.2009.10.024 Google Scholar
  23. Kemper KJ, Small R (1999) Astragalus (Astragalus membranaceous) Longwood Herbal Task Force and The Center for Holistic Pediatric Education and Research. 1–18. http://www.longwoodherbal.org/willowbark/willow.cis.pdf
  24. Ma J, Qiao Z, Xiang X, Qiao Z, Xiang X (2011) Aqueous extract of Astragalus mongholicus ameliorates high cholesterol diet induced oxidative injury in experimental rat models. J Med Plant Res 5(5):855–858Google Scholar
  25. Malir F, Ostry V, Pfohl-Leszkowicz A, Toman J, Bazin I, Roubal T (2014) Transfer of ochratoxin a into tea and coffee beverages. Toxins 6:3438–3453.  https://doi.org/10.3390/toxins6123438 CrossRefPubMedPubMedCentralGoogle Scholar
  26. Ostry V, Malir F, Dofkova M, Skarkova J, Pfohl-Leszkowicz A, Ruprich J (2015) Ochratoxin A dietary exposure of ten population groups in the Czech Republic: comparison with data over the world. Toxins 7:3608–3635.  https://doi.org/10.3390/toxins7093608 CrossRefPubMedPubMedCentralGoogle Scholar
  27. Ostry V, Malir F, Toman J, Grosse Y (2017) Mycotoxins as human carcinogens—the IARC Monographs classification. Mycotoxin Res 33:65–73.  https://doi.org/10.1007/s12550-016-0265-7 CrossRefPubMedGoogle Scholar
  28. Pfohl-Leszkowicz A, Manderville RA (2007) Ochratoxin A: an overview on toxicity and carcinogenicity in animals and humans. Mol Nutr Food Res 51:61–99.  https://doi.org/10.1002/mnfr.200600137 CrossRefPubMedGoogle Scholar
  29. Puntaric D, Bosnir J, Smit Z, Skes I, Baklaic Z (2001) Ochratoxin A in corn and wheat: geographical association with endemic nephropathy. Croat Med J 42:175–180PubMedGoogle Scholar
  30. Rios LJ, Waterman PG (1997) A review of the pharmacology and toxicology of Astragalus. Phytother Res 11:411–418.  https://doi.org/10.1002/(SICI)1099-1573(199709)11:6<411::AID-PTR132>3.0.CO;2-6 CrossRefGoogle Scholar
  31. Roxas M, Jurenka J (2007) Colds and influenza: a review of diagnosis and conventional, botanical, and nutritional considerations. Altern Med Rev 12:25–48PubMedGoogle Scholar
  32. Samson RA, Pitt JI (2000) Integration of modern taxonomic methods for Penicillium and Aspergillus classification. Harwood Academic Publishers, Amsterdam, The Netherlands, pp 1–524Google Scholar
  33. Verotta L, El-Sebakhy N (2001) Cycloartane and oleanane saponins from Astragalus sp. In: Studies in natural products chemistry (Bioactive Natural Products, Part F). Elsevier Science, Karachi, pp 179–234Google Scholar
  34. World Health Organization (WHO) (2013) WHO traditional medicine strategy 2014–2023. WHO, Geneva, SwitzerlandGoogle Scholar
  35. Xu F, Zhang Y, Xiao S, Lu X, Yang D, Yang X, Li C, Shang M, Tu P, Cai S (2006) Absorption and metabolism of Astragali radix decoction: in silico, in vitro, and a case study in vivo. Drug Metab Dispos 34(6):913–924.  https://doi.org/10.1124/dmd.105.008300 PubMedGoogle Scholar
  36. Yang L, Wang L, Pan J, Xiang vL, Yang M, Logrieco AF (2010) Determination of ochratoxin A in traditional Chinese medicinal plants by HPLC-FLD. Food Addit Contam Part A Chem Anal Control Expo Risk Assess 27(7):989–997.  https://doi.org/10.1080/19440041003647122 CrossRefPubMedGoogle Scholar
  37. Zhou W, Kong W, Dou X, Zhao M, Ouyang Z, Yang M (2016) An aptamer based lateral flow strip for on-site rapid detection of ochratoxin A in Astragalus membranaceus. J Chromatogr B Analyt Technol Biomed Life Sci 1022:102–108.  https://doi.org/10.1016/j.jchromb.2016.04.016 CrossRefPubMedGoogle Scholar
  38. Zhu H, Zhang Y, Ye G, Li Z, Zhou P, Huang C (2009) In vivo and in vitro antiviral activities of calycosin-7-O-β-D-glucopyranoside against Coxsackie virus B3. Biol Pharm Bull 32(1):68–73.  https://doi.org/10.1248/bpb.32.68 CrossRefPubMedGoogle Scholar
  39. Zimmerli B, Dick R (1995) Determination of Ochratoxin A at the ppt level in human blood, serum, milk and some foodstuffs by high performance liquid chromatography with enhanced fluorescence detection and immunoaffinity column cleanup: methodology and Swiss data. J. Chromatogr B Biomed Appl 666:85–99CrossRefPubMedGoogle Scholar

Copyright information

© Society for Mycotoxin Research and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Faculty of Science, Department of BiologyUniversity of Hradec KraloveHradec KraloveCzech Republic
  2. 2.Center for Health, Nutrition and FoodNational Institute of Public Health in PragueBrnoCzech Republic
  3. 3.International Agency for Research on CancerLyon CedexFrance
  4. 4.Institute of Public Health in Usti nad LabemRegional Branch Hradec KraloveHradec KraloveCzech Republic

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