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Mycotoxin Research

, Volume 34, Issue 3, pp 211–221 | Cite as

Feasibility of 3D UV-C treatment to reduce fungal growth and mycotoxin loads on maize and wheat kernels

  • Vladimir Popović
  • Nicholas Fairbanks
  • Jacob Pierscianowski
  • Michael Biancaniello
  • Ting Zhou
  • Tatiana Koutchma
Original Article
  • 96 Downloads

Abstract

Fungal disease of grain crops is a concern for the agricultural industry, resulting in economic losses. Aside from severe yield losses, mycotoxigenic fungi such as Penicillium and Fusarium can produce harmful mycotoxins, including deoxynivalenol (DON), zearalenone (ZEN), and ochratoxin A (OTA). This proof-of-concept study explored the feasibility and effects of ultraviolet (UV) C light at 253.7 nm to reduce fungal and mycotoxin loads on model surfaces as well as on maize and wheat kernels using benchtop 2D and 3D illumination strategies. Reduction of Penicillium verrucosum (98.6%) and Fusarium graminearum (88.8%) on agar was achieved using a UV-C dose of 100 mJ cm−2. Naturally occurring fungal growth resembling P. verrucosum on maize was reduced by 79% after exposure to 5000 mJ cm−2. Similarly, fungal growth resembling F. graminearum on maize was reduced by 60% with 1000 mJ cm−2. On wheat, significant reduction of fungal growth was not observed. Maximal reduction of DON (97.3%), ZEN (75.4%), and OTA (91.2%) on filter paper was obtained using 15,000 mJ cm−2. The overall reduction of DON (30%; 14%), ZEN (52%; 42%), and OTA (17%; 6%) on maize and wheat, respectively, was lower than on filter paper. Moisture and crude protein content as well as percent germination of maize kernels were not affected by UV-C treatment up to 5000 mJ cm−2. This study has shown that 3D UV-C treatment is a feasible option for reducing Fusarium and Penicillium growth on maize kernels and, at higher doses, decreasing ZEN by ~ 50%.

Keywords

Ultraviolet light Maize Corn Wheat Fungus Mycotoxins Grains Penicillium Fusarium Deoxynivalenol (DON) Zearalenone (ZEN) Ochratoxin A (OTA) 

Notes

Acknowledgements

The authors would like to thank Don Kabbes from Great Lakes Grains Inc. (Chatham, ON, Canada) for supplying the maize and wheat samples for this study and Yan Zhu for running all HPLC samples.

Funding

This research was funded in equal parts by Agriculture and Agri-Food Canada (Agriculture Innovation Program, AIP) and the Grain Farmers of Ontario (GFO).

Compliance with ethical standards

Conflict of interest

None.

References

  1. Ameer Sumbal G, Hussain Shar Z, Hussain Sherazi ST, Sirajuddin, Nizamani SM, Mahesar SA (2016) Decontamination of poultry feed from ochratoxin A by UV and sunlight radiations. J Sci Food Agric 96:2668–2673.  https://doi.org/10.1002/jsfa.7384 CrossRefPubMedGoogle Scholar
  2. AOAC (2002) official method 992.23: Crude protein in cereal grains and oilseeds. In: Official methods of analysis of AOAC International. AOAC International, Washington. Available (2018-04-19) from http://eoma.aoac.org
  3. Awika JM (2011) Major cereal grains production and use around the world. In: Advances in cereal science: Implications to food processing and health promotion, vol 1089. vol 1089. American Chemical Society, pp 1–13.  https://doi.org/10.1021/bk-2011-1089.ch001
  4. Campbell MA, Medd RW, Brown JB (2003) Optimizing conditions for growth and sporulation of Pyrenophora semeniperda. Plant Pathol 52:448–454.  https://doi.org/10.1046/j.1365-3059.2003.00872.x CrossRefGoogle Scholar
  5. Copeland LO, McDonald MB (1976) Principles of seed science and technology. Burgess, MinneapolisGoogle Scholar
  6. FAO (2017) FAO Cereal Supply and Demand Brief. http://www.fao.org/worldfoodsituation/csdb/en/. Accessed 19 April 2018
  7. Gardner DW, Shama G (2000) Modeling UV-induced inactivation of microorganisms on surfaces. J Food Prot 63:63–70CrossRefPubMedGoogle Scholar
  8. Hidaka Y, Kubota K (2006) Study on the sterilization of grain surface using UV radiation: Development and evaluation of UV irradiation equipment. Jpn Agric Res Q 40:157–161.  https://doi.org/10.6090/jarq.40.157 CrossRefGoogle Scholar
  9. Kuiper-Goodman T, Hilts C, Billiard SM, Kiparissis Y, Richard IDK, Hayward S (2010) Health risk assessment of ochratoxin a for all age-sex strata in a market economy. Food Addit Contam 27:212–240.  https://doi.org/10.1080/02652030903013278 CrossRefGoogle Scholar
  10. Kumar D, Kalita P (2017) Reducing postharvest losses during storage of grain crops to strengthen food security in developing countries. Foods 6:8.  https://doi.org/10.3390/foods6010008 CrossRefPubMedCentralGoogle Scholar
  11. Leach CM (1962) Sporulation of diverse species of fungi under near-ultraviolet radiation. Can J Bot 40:151–161.  https://doi.org/10.1139/b62-016 CrossRefGoogle Scholar
  12. Limay-Rios V, Miller JD, Schaafsma AW (2017) Occurrence of Penicillium verrucosum, ochratoxin A, ochratoxin B and citrinin in on-farm stored winter wheat from the Canadian Great Lakes region. PLoS One 12:e0181239.  https://doi.org/10.1371/journal.pone.0181239 CrossRefPubMedPubMedCentralGoogle Scholar
  13. McMullen M, Jones R, Gallenberg D (1997) Scab of wheat and barley: a re-emerging disease of devastating impact. Plant Dis 81:1340–1348.  https://doi.org/10.1094/PDIS.1997.81.12.1340 CrossRefGoogle Scholar
  14. Murata H, Mitsumatsu M, Shimada N (2008) Reduction of feed-contaminating mycotoxins by ultraviolet irradiation: an in vitro study. Food Addit Contam 25:1107–1110.  https://doi.org/10.1080/02652030802057343 CrossRefGoogle Scholar
  15. Paez C, Reyes M, Aguilar C, Pacheco F, Martinez E, Orea A, Bonilla J (2011) Control of natural mycobiota in maize grains by ultraviolet (UVC) irradiation. Acta Agrophys 18:375–388Google Scholar
  16. Sauer DB, Burroughs R (1986) Disinfection of seed surfaces with sodium hypochlorite. Phytopathology 76:745–749.  https://doi.org/10.1094/Phyto-76-745 CrossRefGoogle Scholar
  17. Schaafsma AW, Phibbs T, Paul D, Tamburic-Ilincic L (2000) Fusarium and mycotoxin contamination of grain by a survey conducted of the Ontario maize crop at harvest in 2000. University of Guelph, RidgetownGoogle Scholar

Copyright information

© Crown 2018

Authors and Affiliations

  • Vladimir Popović
    • 1
  • Nicholas Fairbanks
    • 1
    • 2
  • Jacob Pierscianowski
    • 1
    • 3
  • Michael Biancaniello
    • 1
    • 4
  • Ting Zhou
    • 1
  • Tatiana Koutchma
    • 1
  1. 1.Guelph Research and Development CenterAgriculture and Agri-Food CanadaGuelphCanada
  2. 2.University of VictoriaVictoriaCanada
  3. 3.McMaster UniversityHamiltonCanada
  4. 4.University of GuelphGuelphCanada

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