Abstract
Forty samples of dried vine fruit (raisin, n = 22; currant, n = 18) were collected in 2009–2011 from the Iranian market. Aflatoxins (AFs) and ochratoxin A (OTA) were determined in these samples after immunoaffinity column clean-up by high performance liquid chromatography (HPLC) with fluorescence detection. The limit of quantification (LOQ) for AFs B1. B2, G1, G2, and OTA were 0.62, 0.50, 0.70, 0.40, and 0.42 ng/g, respectively. AFB1 was found in one sample of raisin (0.64 ng/g) and in two samples of currant (0.20 and 0.63 ng/g). AFB2 (0.33 ng/g) and AFG2 (0.49 ng/g) were found in 2 samples of currant. OTA was detected in 3 of the 22 samples of raisin (mean 2.21 ng/g) and in one sample of currant (2.99 ng/g). The results show that in AFs and OTA levels are well below the regulatory limits both of the European Union and of Iran.
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Abdel-Sater MA, Saber SM (1999) Mycoflora and mycotoxins of some Egyptian dried fruits. Bull Fac Sci Assiut 28:91–107
Aksoy U, Eltem R, Meyvaci KB, Altindisli A, Karabat S (2007) Five-year survey of ochratoxin A in processed sultanas from Turkey. Food Addit Contam 24:292–296
Apergi E, Gardikis JP, Panagiotopoulou V-Y (1998) Occurrence of aflatoxins B1, B2, G1 and G2 in imported goods in Greece during 1995. In: Miraglia M, Van Egmond H, Brera C, Gilbert J (eds) Mycotoxins and phycotoxins—developments in chemistry, toxicology and food safety. Alaken, Fort Collins, pp 105–110
Asadi M, Beheshti HR, Feizy J (2011) A survey of aflatoxins in sesame in Iran. Mycotoxin Res 27:259–263
Deiner UL, Cole RJ, Sanders TH, Payne GA, Lee LS, Kich MA (1987) Epidemiology of aflatoxin formation by Aspergillus flavus. Annu Rev Phytopathol 25:240–270
European Commission (2006a) Commission Regulation (EC) No. 401/2006 of 23 February 2006, laying down the methods of sampling and analysis for the official control of the levels of mycotoxins in foodstuffs. Off J Eur Union L 70/12
European Commission (2006b) Commission Regulation EC No. 1881/2006 of 19 December 2006 setting maximum levels for certain contaminants in foodstuffs. Off J Eur Union L 364/5
Fakoor Janati SS, Beheshti HR, Khoshbakht Fahim N, Feizy J (2011) Aflatoxins and ochratoxinin A in bean from Iran. Bull Environ Contam Toxicol 87:194–19
Fakoor Janati SS, Beheshti HR, Asadi M, Mihanparast M, Feizy J (2012) Preliminary survey of aflatoxins and ochratoxin A in dried fruits from Iran. Bull Environ Contam Toxicol 88:391–395
Feizy J, Beheshti HR, Fakoor Janati SS, Khoshbakht Fahim N (2010) Survey of ochratoxin A in rice from Iran using affinity column cleanup and HPLC with fluorescence detection. Food Addit Contam B 3:263–267
Feizy J, Beheshti HR, Fakoor Janati SS, Khoshbakht Fahim N (2011) Survey of aflatoxins in watermelon seeds from Iran using immunoaffinity column cleanup and HPLC with fluorescence detection. Food Addit Contam B 4:106–109
Feizy J, Beheshti HR, Asadi M (2012) A survey of aflatoxin in cotton seed in Iran by HPLC with online photochemical derivatization and fluorescence detection. Food Addit Contam B 5:200–203
Gareis M, Scheuer R (2000) Ochratoxin A in meat and meat products. Arch Leb 51:102–103
Goto T, Ito Y, Peterson SW, Wicklow D (1997) Mycotoxin producing ability of Aspergillus tamarii. Mycotoxin 44:17–20
Guler FK, Heperkan D (2008) Natural occurrence of ochratoxin A in dried figs. Anal Chim Acta 617:32–36
Hubner M, Vrabcheva T, Gareis M (1998) Simultaneous detection of ochratoxin A and B in spices and herbs by immunoaffinity column cleanup and HPLC. Recueil Medecine Veterinaire, p. 507
Iamanaka BT, de-Menezes HC, Vicente E, Leite RSF, Taniwaki MH (2007) Aflatoxigenic fungi and aflatoxins occurrence in sultanas and dried figs commercialized in Brazil. Food Control 18:454–457
Institute of Standards and Industrial Research of Iran (2002) Food and Feed. Mycotoxins: maximum tolerated level. 1st ed. ISIRI No. 5925
International Agency for Research on Cancer (1993) Ochratoxin A. In: IARC Monographs on the evaluation of carcinogenic risk to humans: some naturally is occurring substances, food items and constituents, heterocyclic aromatic amines and mycotoxins, vol 56. InternationalAgency for Research on Cancer, Geneva, pp 26–32
Ito Y, Peterson SW, Wicklow D, Goto T (2001) Aspergillus pseudotamarii, a new aflatoxin producing species in Aspergillus section flavi. Mycol Res 105:233–239
Khoury AE, Rizk T, Lteif R, Azouri H, Delia M, Lebrihi A (2008) Fungal contamination and Aflatoxin B1 and Ochratoxin A in Lebanese wine–grapes and musts. Food ChemToxicol 46:2244–2250
Kutrzman CP, Horn BW, Hesseltine CW (1987) Aspergillus nominus, a new aflatoxin-producing species related to Aspergillus flavus and Aspergillus tamarii. Antonie Van Leeuwenhoek 53:147–158
Lombaert GA, Pellaers P, Neumann G, Kitchen D, Huzel V,Trelka R, Kotello S, Scott PM (2004) Ochratoxin A in driedvine fruits on the Canadian retail market. Food Addit Contam 21:578–585.
MacDonald SJ, Anderson S, Brereton P, Wood R (2003) Determination of ochratoxin A in currants, raisins, sultanas, mixed dried fruit, and dried figs by immunoaffinity column clean-up with liquid chromatography: interlaboratory study. J AOAC Int 86:1164–1171
Meyvaci KB, Altindisli A, Aksoy U, Eltem R, Turgut H, Arasiler Z, Kartal N (2005) Ochratoxin A in sultanas from Turkey I: survey of unprocessed sultanas from vineyards and packing-houses. Food Addit Contam 22:1138–1143
Moller TE, Nyberg M (2003) Ochratoxin A in raisins, & currants: basic extraction procedure used in two small marketing surveys of the occurrence and control of the heterogeneity of the toxins in samples. Food Addit Contam 20:1072–1076
Pitt DB, Plestina JI, Shepard R, Solfrizzo G, Vergere M, Walker PJP (2001) Safety evaluation of certain mycotoxins in food. In: Joint FAO/WHO Expert Committee on Food Additives (JECFA), vol 47. Food and Agriculture Organization, Rome, pp 281–415
Rodrigues LC, Camppa Ta AG, Linares CJ, Ceba MR (1993) Estimation of performace characteristics an analytical method using the data set of the calibration experiment. Anal Lett 26(6):1243–1258
Sage L, Krivobok S, Delbos E, Seigle-Murandi F, Creppy EE (2002) Fungal flora and Ochratoxin A production in grapes and musts from France. J Agric Food Chem 50:1306–1311
Saxena J, Mehrotra BS (1990) The occurrence of mycotoxins in some dry fruits retail marketed in Nainital district of India. Acta Aliment Hung 19:221–224
Senyuva HZ, Gilbert J, Ozcan S, Ulken U (2005) Survey for co-occurrence of ochratoxin A and aflatoxin B1 in dried figs in Turkey by using a single laboratory-validated alkaline extraction method for ochratoxin A. J Food Prot 68:1512–1515
Trucksess MW, Scott PM (2008) Mycotoxins inbotanicals and dried fruits: a review. Food Addit Contam 25:181–192
Trucksess MW, Giler J, Young K, White KD, Page SW (1999) Determination and survey of ochratoxin A in wheat, barley and coffee, 1997. J AOAC Int 82:85–89
Varga J, Kocsubé S, Koncz Z, Téren J (2006) MycobiOchratoxin A and ochratoxin A in raisins purchased in Hungary. Acta Aliment Hung 35:289–294
Youssef MS, Abo-Dahab NF, Abou-Seidah AA (2000) Mycobiota and mycotoxin contamination of dried raisins in Egypt. Afr J Mycol Biotechnol 8:69–86, Chemical Abstracts 135: 317617r, 2001
Zinedine A, Soriano JM, Juan C, Mojemmi B et al (2007) Incidence of ochratoxin A in rice and dried fruits from Rabat and Sale area, Morocco. Food Addit Contam 24:285–291
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Feizy, J., Beheshti, H.R. & Asadi, M. Ochratoxin A and aflatoxins in dried vine fruits from the Iranian market. Mycotoxin Res 28, 237–242 (2012). https://doi.org/10.1007/s12550-012-0145-8
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DOI: https://doi.org/10.1007/s12550-012-0145-8