Mycotoxin Research

, Volume 27, Issue 2, pp 105–113 | Cite as

Deoxynivalenol loads in matched pair wheat samples in Belgium: comparison of ELISA VERATOX kit against liquid chromatography

  • Emmanuel K. Tangni
  • Jean-Claude Motte
  • Alfons Callebaut
  • Anne Chandelier
  • Marnix De Schrijver
  • Luc Pussemier
Original Paper

Abstract

A comparison of matched pairs deoxynivalenol (DON) loads in wheat samples via VERATOX for DON 5/5 performed by two laboratories against two liquid chromatographic methods (LC-MS/MS and HPLC-UV) used by two other laboratories was carried out using biometrical and sum of ranking differences (SRD) procedures. The Lin’s Concordance correlation coefficients, the average discrepancies, the limits of agreement and the SRD between ELISA and reference values showed good overall agreement between VERATOX for DON 5/5 and reference methods for the two datasets. The VERATOX kits are valuable for quantitative screening and even for an initial exposure assessment in situations when there are practical or economical reasons not to use sophisticated methods such as HPLC or GC methods (with or without MS). However, networking of laboratories using this rapid method and laboratories with reference analytical methods should be encouraged.

Keywords

Deoxynivalenol Wheat VERATOX Liquid chromatography Confirmation Agreement 

Abbreviations

CCC

Lin’s concordance correlation coefficient

CRRN

Compare of Ranks with Random Numbers

ELISA

Enzyme-linked immunosorbent assay

HPLC

High pressure liquid chromatography

LOA

Limits of agreement

RMSE

Root of mean square error

SRD

Sum of ranking differences

SRDnormed

Normalized SRD values

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Copyright information

© Society for Mycotoxin Research and Springer 2010

Authors and Affiliations

  • Emmanuel K. Tangni
    • 1
  • Jean-Claude Motte
    • 1
  • Alfons Callebaut
    • 1
  • Anne Chandelier
    • 2
  • Marnix De Schrijver
    • 3
  • Luc Pussemier
    • 1
  1. 1.Chemical Safety of the Food Chain, Unit of Toxins and Natural componentsVeterinary and Agrochemical Research Centre (CODA-CERVA)TervurenBelgium
  2. 2.Department Biological Control and Plant Genetic ResourcesWalloon Agricultural Research Centre (CRA-W)GemblouxBelgium
  3. 3.Food and Feed Quality (FFQ) LaboratoriumMerksemBelgium

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