A review on 3D printed bioimplants

  • Chee Meng Benjamin Ho
  • Sum Huan Ng
  • Yong-Jin Yoon


Additive manufacturing (AM) also known as 3D printing have been making inroads into medical applications such as surgical models and tools, tooling equipment, medical devices. One key area researchers are looking into is bioimplants. With the improvement and development of AM technologies, many different bioimplants can be made using 3D printing. Different biomaterials and various AM technologies can be used to create customized bioimplants to suit the individual needs. With the aid of 3D printing this could lead to new foam and design of bioimplants in the near further. Therefore, the purpose of this review articles is to (1) Describe the various AM technologies and process used to make bioimplants, (2) Different types of bioimplants printed with AM and (3) Discuss some of the challenges and future developments for 3D printed bioimplants.


3D printing Additive manufacturing Bioimplant 


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  1. 1.
    DGBMT, “Bioimplants: Biological, Biologised and Biofunctionalised Implants,” pdf (Accessed 1 APR 2015)Google Scholar
  2. 2.
    Wohlers, T. T., “Wohlers Report 2013: Additive Manufacturing and 3D Printing State of the Industry: Annual Worldwide Progress Report”, 2013.Google Scholar
  3. 3.
    Salmi, M., Tuomi, J., Paloheimo, K.-S., Björkstrand, R., Paloheimo, M., et al., “Patient-Specific Reconstruction with 3D Modeling and DMLS Additive Manufacturing,” Rapid Prototyping Journal, Vol. 18, No. 3, pp. 209–214, 2012.CrossRefGoogle Scholar
  4. 4.
    Jakab, K., Norotte, C., Marga, F., Murphy, K., Vunjak-Novakovic, G., and Forgacs, G., “Tissue Engineering by Self-Assembly and Bio-Printing of Living Cells,” Biofabrication, Vol. 2, No. 2, Paper No. 022001, 2010.Google Scholar
  5. 5.
    Warnke, P. H., Seitz, H., Warnke, F., Becker, S. T., Sivananthan, S., et al., “Ceramic Scaffolds Produced by Computer-Assisted 3D Printing and Sintering: Characterization and Biocompatibility Investigations,” Journal of Biomedical Materials Research Part B: Applied Biomaterials, Vol. 93, No. 1, pp. 212–217, 2010.Google Scholar
  6. 6.
    Kruth, J., Vandenbroucke, B., Van Vaerenbergh, J., and Naert, I., “Digital Manufacturing of Biocompatible Metal Frameworks for Complex Dental Prostheses by Means of SLS/SLM,” Virtual Prototyping and Rapid Manufacturing-Advanced research in virtual and Rapid Prototyping, Taylor & Francis, pp. 139–146, 2005.Google Scholar
  7. 7.
    Lavine, M., Roberts, L., and Smith, O., “If I Only Had a,” Science, Vol. 295, No. 5557, p. 995, 2002.CrossRefGoogle Scholar
  8. 8.
    Kolan, K. C., Leu, M. C., Hilmas, G. E., Brown, R. F., and Velez, M., “Fabrication of 13-93 Bioactive Glass Scaffolds for Bone Tissue Engineering using Indirect Selective Laser Sintering,” Biofabrication, Vol. 3, No. 2, Paper No. 025004, 2011.Google Scholar
  9. 9.
    U.S. Food and Drug Administration, “IDE Definitions and Acronyms,” ExemptionIDE/ucm046698.htm (Accessed 1 APR 2015)Google Scholar
  10. 10.
    Giannatsis, J. and Dedoussis, V., “Additive Fabrication Technologies Applied to Medicine and Health Care: A Review,” The International Journal of Advanced Manufacturing Technology, Vol. 40, No. 1–2, pp. 116–127, 2009.CrossRefGoogle Scholar
  11. 11.
    Webb, P., “A Review of Rapid Prototyping (RP) Techniques in the Medical and Biomedical Sector,” Journal of Medical Engineering & Technology, Vol. 24, No. 4, pp. 149–153, 2000.CrossRefGoogle Scholar
  12. 12.
    Melchels, F. P., Domingos, M. A., Klein, T. J., Malda, J., Bartolo, P. J., and Hutmacher, D. W., “Additive Manufacturing of Tissues and Organs,” Progress in Polymer Science, Vol. 37, No. 8, pp. 1079–1104, 2012.CrossRefGoogle Scholar
  13. 13.
    Honiball, J. R., “The Application of 3D Printing in Reconstructive Surgery,” Stellenbosch: University of Stellenbosch, 2010.Google Scholar
  14. 14.
    Bourell, D. L., Leu, M. C., and Rosen, D. W., “Roadmap for Additive Manufacturing: Identifying the Future of Freeform Processing,” The University of Texas at Austin, 2009.Google Scholar
  15. 15.
    Chua, C. K., Leong, K. F., and Lim, C. S., “Rapid Prototyping: Principles and Applications,” World Scientific, 2010.Google Scholar
  16. 16.
    Rezende, R. A., Pereira, F. D., Kasyanov, V., Ovsianikov, A., Torgensen, J., et al., “Design, Physical Prototyping and Initial Characterisation of ‘Lockyballs’ this Paper Reports the Fabrication of Interlockable Microscale Scaffolds using Two Photon Polymerization (2pp) and Proposes a “Lockyball” Approach for Tissue Self-Assembly for Biofabrication,” Virtual and Physical Prototyping, Vol. 7, No. 4, pp. 287–301, 2012.CrossRefGoogle Scholar
  17. 17.
    Ferris, C. J., Gilmore, K. G., and Wallace, G. G., “Biofabrication: An Overview of the Approaches Used for Printing of Living Cells,” Applied Microbiology and Biotechnology, Vol. 97, No. 10, pp. 4243–4258, 2013.CrossRefGoogle Scholar
  18. 18.
    Peltola, S. M., Melchels, F. P., Grijpma, D. W., and Kellomäki, M., “A Review of Rapid Prototyping Techniques for Tissue Engineering Purposes,” Annals of Medicine, Vol. 40, No. 4, pp. 268–280, 2008.CrossRefGoogle Scholar
  19. 19.
    Yeong, W. Y., Chua, C. K., Leong, K. F., and Chandrasekaran, M., “Rapid Prototyping in Tissue Engineering: Challenges and Potential,” Trends in Biotechnology, Vol. 22, No. 12, pp. 643–652, 2004.CrossRefGoogle Scholar
  20. 20.
    Schiele, N. R., Corr, D. T., Huang, Y., Raof, N. A., Xie, Y., and Chrisey, D. B., “Laser-based Direct-Write Techniques for Cell Printing,” Biofabrication, Vol. 2, No. 3, Paper No. 032001, 2010.Google Scholar
  21. 21.
    Eyers, D. and Dotchev, K., “Technology Review for Mass Customisation using Rapid Manufacturing,” Assembly Automation, Vol. 30, No. 1, pp. 39–46, 2010.CrossRefGoogle Scholar
  22. 22.
    He, J., Li, D., Lu, B., Wang, Z., and Zhang, T., “Custom Fabrication of a Composite Hemi-Knee Joint based on Rapid Prototyping,” Rapid Prototyping Journal, Vol. 12, No. 4, pp. 198–205, 2006.CrossRefGoogle Scholar
  23. 23.
    Singare, S., Yaxiong, L., Dichen, L., Bingheng, L., Sanhu, H., and Gang, L., “Fabrication of Customised Maxillo-Facial Prosthesis using Computer-Aided Design and Rapid Prototyping Techniques,” Rapid Prototyping Journal, Vol. 12, No. 4, pp. 206–213, 2006.CrossRefGoogle Scholar
  24. 24.
    Chang, C., Lee, M., and Wang, S., “Digital Denture Manufacturing- An Integrated Technologies of Abrasive Computer Tomography, CNC Machining and Rapid Prototyping,” The International Journal of Advanced Manufacturing Technology, Vol. 31, No. 1–2, pp. 41–49, 2006.CrossRefGoogle Scholar
  25. 25.
    Eggbeer, D., Bibb, R., and Williams, R., “The Computer-Aided Design and Rapid Prototyping Fabrication of Removable Partial Denture Frameworks,” Proceedings of the Institution of Mechanical Engineers, Part H: Journal of Engineering in Medicine, Vol. 219, No. 3, pp. 195–202, 2005.CrossRefGoogle Scholar
  26. 26.
    Ovsianikov, A., Chichkov, B., Adunka, O., Pillsbury, H., Doraiswamy, A., and Narayan, R., “Rapid Prototyping of Ossicular Replacement Prostheses,” Applied Surface Science, Vol. 253, No. 15, pp. 6603–6607, 2007.CrossRefGoogle Scholar
  27. 27.
    Jardini, A., Larosa, M., Bernardes, L., Zavaglia, C., and MacielFilho, R., “Application of Direct Metal Laser Sintering in Titanium Alloy For Cranioplasty,” Proc. of 6th Brazilian Conference on Manufacturing Engineering, 2011.Google Scholar
  28. 28.
    Bibb, R., Eggbeer, D., and Williams, R., “Rapid Manufacture of Removable Partial Denture Frameworks,” Rapid Prototyping Journal, Vol. 12, No. 2, pp. 95–99, 2006.CrossRefGoogle Scholar
  29. 29.
    Vandenbroucke, B. and Kruth, J. P., “Selective Laser Melting of Biocompatible Metals for Rapid Manufacturing of Medical Parts,” Rapid Prototyping Journal, Vol. 13, No. 4, pp. 196–203, 2007.CrossRefGoogle Scholar
  30. 30.
    Hollander, D. A., Von Walter, M., Wirtz, T., Sellei, R., Schmidt-Rohlfing, B.,et al., “Structural, Mechanical and in Vitro Characterization of Individually Structured Ti-6Al-4V Produced by Direct Laser Forming,” Biomaterials, Vol. 27, No. 7, pp. 955–963, 2006.CrossRefGoogle Scholar
  31. 31.
    Li, X., Wang, J., Shaw, L. L., and Cameron, T. B., “Laser Densification of Extruded Dental Porcelain Bodies in Multi-Material Laser Densification Process,” Rapid Prototyping Journal, Vol. 11, No. 1, pp. 52–58, 2005.CrossRefGoogle Scholar
  32. 32.
    Meszaros, R., Zhao, R., Travitzky, N., Fey, T., Greil, P., and Wondraczek, L., “Three-Dimensional Printing of a Bioactive Glass,” Glass Technology-European Journal of Glass Science and Technology Part A, Vol. 52, No. 4, pp. 111–116, 2011.Google Scholar
  33. 33.
    Harrysson, O. L., Cansizoglu, O., Marcellin-Little, D. J., Cormier, D. R., and West, H. A., “Direct Metal Fabrication of Titanium Implants with Tailored Materials and Mechanical Properties using Electron Beam Melting Technology,” Materials Science and Engineering: C, Vol. 28, No. 3, pp. 366–373, 2008.CrossRefGoogle Scholar
  34. 34.
    Yang, J., Cai, H., Lv, J., Zhang, K., Leng, H.,et al., “In Vivo Study of a Self-Stabilizing Artificial Vertebral Body Fabricated by Electron Beam Melting,” Spine, Vol. 39, No. 8, pp. E486–E492, 2014.Google Scholar
  35. 35.
    Vorndran, E., Klammert, U., Ewald, A., Barralet, J. E., and Gbureck, U., “Simultaneous Immobilization of Bioactives during 3D Powder Printing of Bioceramic Drug-Release Matrices,” Advanced Functional Materials, Vol. 20, No. 10, pp. 1585–1591, 2010.CrossRefGoogle Scholar
  36. 36.
    Ciocca, L. and Scotti, R., “CAD-CAM Generated Ear Cast by Means of a Laser Scanner and Rapid Prototyping Machine,” The Journal of Prosthetic Dentistry, Vol. 92, No. 6, pp. 591–595, 2004.CrossRefGoogle Scholar
  37. 37.
    Murphy, S. V. and Atala, A., “3D Bioprinting of Tissues and Organs,” Nature Biotechnology, Vol. 32, No. 8, pp. 773–785, 2014.CrossRefGoogle Scholar
  38. 38.
    Cui, X., Breitenkamp, K., Finn, M., Lotz, M., and D’Lima, D. D., “Direct Human Cartilage Repair using Three-Dimensional Bioprinting Technology,” Tissue Engineering Part A, Vol. 18, No. 11–12, pp. 1304–1312, 2012.CrossRefGoogle Scholar
  39. 39.
    Struecker, B., Raschzok, N., and Sauer, I. M., “Liver Support Strategies: Cutting-Edge Technologies,” Nature Reviews Gastroenterology & Hepatology, Vol. 11, No. 3, pp. 166–176, 2014.CrossRefGoogle Scholar
  40. 40.
    Chang, R., Emami, K., Wu, H., and Sun, W., “Biofabrication of a Three-Dimensional Liver Micro-Organ as an In Vitro Drug Metabolism Model,” Biofabrication, Vol. 2, No. 4, Paper No. 045004, 2010.Google Scholar
  41. 41.
    Snyder, J., Hamid, Q., Wang, C., Chang, R., Emami, K., et al., “Bioprinting Cell-Laden Matrigel for Radioprotection Study of Liver By Pro-Drug Conversion in a Dual-Tissue Microfluidic Chip,” Biofabrication, Vol. 3, No. 3, Paper No. 034112, 2011.Google Scholar
  42. 42.
    Jakab, K., Norotte, C., Damon, B., Marga, F., Neagu, A., et al., “Tissue Engineering by Self-Assembly of Cells Printed into Topologically Defined Structures,” Tissue Engineering Part A, Vol. 14, No. 3, pp. 413–421, 2008.CrossRefGoogle Scholar
  43. 43.
    Khatiwala, C., Law, R., Shepherd, B., Dorfman, S., and Csete, M., “3D Cell Bioprinting for Regenerative Medicine Research and Therapies,” Gene Therapy and Regulation, Vol. 7, No. 1, Paper No. 1230004, 2012.Google Scholar
  44. 44.
    Koch, L., Kuhn, S., Sorg, H., Gruene, M., Schlie, S., et al., “Laser Printing of Skin Cells and Human Stem Cells,” Tissue Engineering Part C: Methods, Vol. 16, No. 5, pp. 847–854, 2009.CrossRefGoogle Scholar
  45. 45.
    Gruene, M., Deiwick, A., Koch, L., Schlie, S., Unger, C., et al., “Laser Printing of Stem Cells for Biofabrication of Scaffold-Free Autologous Grafts,” Tissue Engineering Part C: Methods, Vol. 17, No. 1, pp. 79–87, 2010.CrossRefGoogle Scholar
  46. 46.
    Gaebel, R., Ma, N., Liu, J., Guan, J., Koch, L., et al., “Patterning Human Stem Cells and Endothelial Cells with Laser Printing for Cardiac Regeneration,” Biomaterials, Vol. 32, No. 35, pp. 9218–9230, 2011.CrossRefGoogle Scholar
  47. 47.
    Syam, W. P., Mannan, M., and Al-Ahmari, A., “Rapid Prototyping and Rapid Manufacturing in Medicine and Dentistry: This Paper Presents an Overview of Recent Developments in the Field of Rapid Prototyping and Rapid Manufacturing with Special Emphasis in Medicine and Dentistry,” Virtual and Physical Prototyping, Vol. 6, No. 2, pp. 79–109, 2011.CrossRefGoogle Scholar
  48. 48.
    Ozbolat, I. T. and Yu, Y., “Bioprinting Toward Organ Fabrication: Challenges and Future Trends,” IEEE Transactions on Biomedical Engineering, Vol. 60, No. 3, pp. 691–699, 2013.CrossRefGoogle Scholar
  49. 49.
    Al Mardini, M., Ercoli, C., and Graser, G. N., “A Technique to Produce a Mirror-Image Wax Pattern of an Ear using Rapid Prototyping Technology,” The Journal of prosthetic Dentistry, Vol. 94, No. 2, pp. 195–198, 2005.CrossRefGoogle Scholar
  50. 50.
    Liu, Q., Leu, M. C., and Schmitt, S. M., “Rapid Prototyping in Dentistry: Technology and Application,” The International Journal of Advanced Manufacturing Technology, Vol. 29, No. 3–4, pp. 317–335, 2006.CrossRefGoogle Scholar
  51. 51.
    Wu, M., Tinschert, J., Augthun, M., Wagner, I., Schädlich-Stubenrauch, J., et al., “Application of Laser Measuring, Numerical Simulation and Rapid Prototyping to Titanium Dental Castings,” Dental Materials, Vol. 17, No. 2, pp. 102–108, 2001.CrossRefGoogle Scholar
  52. 52.
    Leong, K. F., Chua, C. K., and Gui, W. S., “Building Porous Biopolymeric Microstructures for Controlled Drug Delivery Devices using Selective Laser Sintering,” The International Journal of Advanced Manufacturing Technology, Vol. 31, No. 5–6, pp. 483–489, 2006.CrossRefGoogle Scholar
  53. 53.
    Low, K., Leong, K., Chua, C., Du, Z., and Cheah, C., “Characterization of SLS Parts for Drug Delivery Devices,” Rapid Prototyping Journal, Vol. 7, No. 5, pp. 262–268, 2001.CrossRefGoogle Scholar
  54. 54.
    Manzano, M. and Vallet-Regí, M., “Revisiting Bioceramics: Bone Regenerative and Local Drug Delivery Systems,” Progress in Solid State Chemistry, Vol. 40, No. 3, pp. 17–30, 2012.CrossRefGoogle Scholar
  55. 55.
    Wu, C., Luo, Y., Cuniberti, G., Xiao, Y., and Gelinsky, M., “Three- Dimensional Printing of Hierarchical and Tough Mesoporous Bioactive Glass Scaffolds with a Controllable Pore Architecture, Excellent Mechanical Strength and Mineralization Ability,” Acta Biomaterialia, Vol. 7, No. 6, pp. 2644–2650, 2011.CrossRefGoogle Scholar
  56. 56.
    Sharp, P., Cooney, C., Kastner, M., Lees, J., Sasisekharan, R., et al., “The Third Revolution: The Convergence of the Life Sciences, Physical Sciences, and Engineering,” Massachusetts Institute of Technology, 2011.Google Scholar
  57. 57.
    Sharp, P. A. and Langer, R., “Promoting Convergence in Biomedical Science,” Science, Vol. 333, No. 6042, p. 527, 2011.CrossRefGoogle Scholar
  58. 58.
    Bens, A., Seitz, H., Bermes, G., Emons, M., Pansky, A., et al., “Non-Toxic Flexible Photopolymers for Medical Stereolithography Technology,” Rapid Prototyping Journal, Vol. 13, No. 1, pp. 38–47, 2007.CrossRefGoogle Scholar
  59. 59.
    Chou, D. T., Wells, D., Hong, D., Lee, B., Kuhn, H., and Kumta, P. N., “Novel Processing of Iron–Manganese Alloy-based Biomaterials by Inkjet 3-D Printing,” Acta Biomaterialia, Vol. 9, No. 10, pp. 8593–8603, 2013.CrossRefGoogle Scholar
  60. 60.
    Balandin, A. A., Ghosh, S., Bao, W., Calizo, I., Teweldebrhan, D., et al., “Superior Thermal Conductivity of Single-Layer Graphene,” Nano Letters, Vol. 8, No. 3, pp. 902–907, 2008.CrossRefGoogle Scholar
  61. 61.
    Lee, C., Wei, X., Kysar, J. W., and Hone, J., “Measurement of the Elastic Properties and Intrinsic Strength of Monolayer Graphene,” Science, Vol. 321, No. 5887, pp. 385–388, 2008.CrossRefGoogle Scholar
  62. 62.
    Novoselov, K. S., Geim, A. K., Morozov, S., Jiang, D., Zhang, Y., et al., “Electric Field Effect in Atomically Thin Carbon Films,” Science, Vol. 306, No. 5696, pp. 666–669, 2004.CrossRefGoogle Scholar
  63. 63.
    Bunch, J. S., Verbridge, S. S., Alden, J. S., van der Zande, A. M., Parpia, J. M., et al., “Impermeable Atomic Membranes from Graphene Sheets,” Nano Letters, Vol. 8, No. 8, pp. 2458–2462, 2008.CrossRefGoogle Scholar
  64. 64.
    Tölle, F. J., Fabritius, M., and Mülhaupt, R., “Emulsifier-Free Graphene Dispersions with High Graphene Content for Printed Electronics and Freestanding Graphene Films,” Advanced Functional Materials, Vol. 22, No. 6, pp. 1136–1144, 2012.CrossRefGoogle Scholar
  65. 65.
    Ladd, C., So, J. H., Muth, J., and Dickey, M. D., “3D Printing of Free Standing Liquid Metal Microstructures,” Advanced Materials, Vol. 25, No. 36, pp. 5081–5085, 2013.CrossRefGoogle Scholar
  66. 66.
    Stratasys, “Stratasys Design Series,” (Accessed 1 APR 2015)Google Scholar
  67. 67.
    Mustaffa, B., Narahara, H., and Suzuki, H., “Electronic Circuit Fabrication using Inkjet Printed Silver Nanoparticles for SFF,” Proc. of Japan Society for Precision Engineering National Conference, pp. 83–84, 2004.Google Scholar
  68. 68.
    3D Printing Industry, “3D Printing Breakthrough Yields Organic and Inorganic Multimaterial Vista Printhead,” (Accessed 1 APR 2015)Google Scholar
  69. 69.
    Mannoor, M. S., Jiang, Z., James, T., Kong, Y. L., Malatesta, K. A., et al., “3D Printed Bionic Ears,” Nano Letters, Vol. 13, No. 6, pp. 2634–2639, 2013.CrossRefGoogle Scholar
  70. 70.
    Khalil, S., Nam, J., and Sun, W., “Multi-Nozzle Deposition for Construction of 3D Biopolymer Tissue Scaffolds,” Rapid Prototyping Journal, Vol. 11, No. 1, pp. 9–17, 2005.CrossRefGoogle Scholar
  71. 71.
    Khalil, S., Nam, J., and Sun, W., “Multi-Nozzle Deposition for Construction of 3D Biopolymer Tissue Scaffolds,” Rapid Prototyping Journal, Vol. 11, No. 1, pp. 9–17, 2006.CrossRefGoogle Scholar

Copyright information

© Korean Society for Precision Engineering and Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Chee Meng Benjamin Ho
    • 1
    • 2
    • 3
  • Sum Huan Ng
    • 3
  • Yong-Jin Yoon
    • 1
    • 2
  1. 1.School of Mechanical and Aerospace EngineeringNanyang Technological UniversitySingapore CitySingapore
  2. 2.NTU Additive Manufacturing CentreNanyang Technological UniversitySingapore CitySingapore
  3. 3.A*STAR’s Singapore Institute of Manufacturing Technology (SIMTech)Singapore CitySingapore

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