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Comparative Analysis of CRISPR Loci Found in Streptomyces Genome Sequences

  • Jinqi Zhang
  • Xiaobin Li
  • Zixin Deng
  • Hong-Yu Ou
Original Research Article

Abstract

The interspaced short palindromic repeats (CRISPR) system is an immune system widely distributed in prokaryotes, resisting the invasion of the foreign mobile genetic elements like phages or plasmids. In this study, we present the comparative analysis of 182 CRISPR loci found in 46 publicly available complete genome sequences of Streptomyces. Overall, nine direct repeats (DRs) groups are identified while all the 2104 spacers are divided into three main groups according to the multiple sequence alignment. Only 11 spacers are identical with parts of 10 plasmid sequences, which indicates a possible origin. The cas gene clusters near the CRISPR arrays are found to mainly belong to the I–E subtype. These CRISPR loci might play an important role in the genome evolution of Streptomyces.

Keywords

Streptomyces CRISPR loci Direct repeats Spacers Cas gene cluster 

Notes

Acknowledgements

This work was supported by the National Key R&D Program of China [2017YFC1600100 to H.Y.O.] and the National Natural Science Foundation of China [31371261 to H.Y.O., 21661140002 to Z.D.].

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflicts of interest with the contents of this article.

Supplementary material

12539_2018_301_MOESM1_ESM.pdf (540 kb)
Supplementary material 1 (PDF 541 KB)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.State Key Laboratory of Microbial Metabolism, Joint International Laboratory on Metabolic and Developmental Sciences, School of Life Sciences and BiotechnologyShanghai Jiao Tong UniversityShanghaiChina

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