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Molecular confirmation of hybridization between Dascyllus reticulatus × Dascyllus aruanus from the Great Barrier Reef

  • Song He
  • Jacob L. Johansen
  • Andrew S. Hoey
  • Melissa K. Pappas
  • Michael L. Berumen
Original Paper

Abstract

To date, more than 81 species of tropical coral reef fish have been reported to hybridize in nature, spanning multiple families, including the Chaetodontidae, Pomacanthidae, and Labridae. Hybridization, however, is seemingly rare among benthic nesting species that engage in pair spawning, such as the Pomacentridae. Here, we present evidence for the first molecularly confirmed record of hybridization within the genus Dascyllus; D. aruanus and D. reticulatus. Interestingly, although many hybridization events are attributed to peripheral range effects or areas of limited overlap among otherwise allopatric species, this hybrid individual was collected from the northern Great Barrier Reef, centrally located within the distribution ranges of both species. The hybrid exhibited coloration and meristic counts intermediate between D. aruanus and D. reticulatus. Diagnostic genetic markers and subsequent microsatellites analysis confirmed that this individual was a hybrid offspring of D. aruanus and D. reticulatus, with the latter providing the maternal contribution. The occurrence of the D. aruanus × D. reticulatus hybrid on the Great Barrier Reef represents an exception to the otherwise species-specific haplotypes. The nuclear diagnostic marker which was identified during this study could serve as a hybrid indicator and benefit future hybrid investigations for hybridization between these two species.

Keywords

Damselfish Natural hybridization Molecular identification 

Notes

Acknowledgements

This study was conducted under Great Barrier Reef Marine Park Authority permit G11/34319.1 and QLD Fisheries permit 103256. Ethical approval was obtained from James Cook University (permit A1267). We thank the Lizard Island Research Station staff for fieldwork support and the King Abdullah University of Science and Technology (KAUST) Bioscience Core Laboratory for laboratory support. We thank Gerry Allen, Tane Sinclair-Taylor, and Jean-François Flot for the useful discussions and/or assistance with figures. We thank Prof. Zeng Xiaoqi from the Ocean University of China for sharing the samples collected from Paracel Islands, South China Sea. Financial support was provided by the Australian Research Council (DE130100688 to ASH) and KAUST baseline research funds (to MLB).

Supplementary material

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ESM 1 (DOCX 1032 kb)
12526_2017_819_MOESM2_ESM.xlsx (58 kb)
ESM 2 (XLSX 58 kb)

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Copyright information

© Senckenberg Gesellschaft für Naturforschung and Springer-Verlag GmbH Germany, part of Springer Nature 2017

Authors and Affiliations

  1. 1.Red Sea Research Center, Division of Biological and Environmental Science and EngineeringKing Abdullah University of Science and TechnologyThuwalSaudi Arabia
  2. 2.Marine Science InstituteUniversity of Texas at AustinAustinUSA
  3. 3.ARC Centre of Excellence for Coral Reef StudiesJames Cook UniversityTownsvilleAustralia

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