Isolation and characterisation of 16 polymorphic microsatellite loci for the sooty tern (Onychoprion fuscatus; Sternidae), a super-abundant pan-tropical seabird, including a test of cross-species amplification using two noddies (Anous spp.)
- 120 Downloads
We isolate and characterise 16 polymorphic microsatellite loci for the super-abundant, pan-tropical sooty tern (Onychoprion fuscatus), facilitating population genetic studies. In 70 samples from two breeding colonies, the total number of alleles per locus ranged between 5 and 21, observed heterozygosity ranged from 0.143 to 0.942, while estimated null allele frequency varied from −0.131 to 0.273. Polymerase chain reaction (PCR) conditions were optimised across loci, enabling multiplexing and rapid multilocus genotyping. These 16 loci will be useful for future studies of genetic diversity and population structure, and can be used as a proxy through which to assess ecosystem function and change. We additionally test cross-species amplification in the brown (Anous stolidus) and lesser (A. tenuirostris) noddies, illustrating a use of these microsatellites in other related Sternidae species.
KeywordsSooty tern Onychoprion fuscatus Anous Sternidae Microsatellite Population genetics Conservation
Primer development was co-funded by the Western Indian Ocean Marine Science Association (WIOMSA; grant MARG I_2015_03) and the South African Research Chairs Initiative (SARChI) of the South African Department of Science and Technology (DST) and the National Research Foundation (NRF). We are additionally grateful to Raylene Swanepoel for technical support, and to Matthieu Bastien, Sophie Bureau, Chris Feare, Audrey Jaeger and Christine Larose for sample collection. Extreme gratitude must also be paid to the Savy family, for providing access and support at Bird Island. All procedures performed in this study were in accordance with the ethical standards of the institutions (Department of Zoology and Entomology, Rhodes University, Animal Ethics reference number: ZOOL-01-2013) and organisations through which it was conducted. Finally, the authors declare that they have no conflict of interest.
- Gochfeld M, Burger J (1996) Family Sternidae (terns). In: del Hoyo J, Elliott A, Sargatal J (eds) Handbook of birds of the world. Lynx Edicions, Barcelona, pp 624–667Google Scholar
- Lebarbenchon C, Jaeger A, Feare C, Bastien M, Dietrich M, Larose C, Lagadec E, Rocamora G, Shah N, Pascalis H, Boulinier T, Le Corre M, Stallknecht DE, Dellagi K (2015) Influenza a virus on oceanic islands: host and viral diversity in seabirds in the western Indian Ocean. PLoS Pathog 11(5):e1004925CrossRefGoogle Scholar
- Paradis E (2010) Peags: an R package for population genetics with an integrated-modular approach. Bioinformatics 26:419–420Google Scholar
- R Development Core Team (2017) R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. http://www.R-project.org/, accessed 20 Mar 2017
- Schreiber EA, Feare CJ, Harrington B, Murray B, Robertson WB, Robertson B, Woolfenden GE (2002) Sooty tern Sterna fuscata. In: Poole A, Gill F (eds) The birds of North America. The Birds of North America, Philadelphia, p 32Google Scholar
- Surman CA, Nicholson LW (2009) The good, bad and the ugly. ENSO driven oceanographic variability and its influence on seabird diet and reproductive performance at the Houtman Abrolhos, eastern Indian Ocean. Mar Ornithol 37:129–138Google Scholar