Highly polymorphic microsatellite loci for the Acapulco damselfish, Stegastes acapulcoensis, and cross amplification in three congeneric species
In the present study we report a set of 13 novel microsatellites isolated from and characterized for the Acapulco damselfish Stegastes acapulcoensis, a species endemic to, but widely distributed within, the Eastern Tropical Pacific (ETP) region. The loci were tested in 30 individuals from the Colombian Pacific and were highly polymorphic. The mean allele number per locus was 19.5 (± 4.03 SD) and the observed heterozygosities ranged from 0.192 to 1. Some of the loci were successfully cross-amplified and were polymorphic in three Stegastes species from the ETP: S. arcifrons, S. flavilatus and S. beebei (with 11, three and seven amplified loci, respectively). The high variability and cross-amplification success of the new set of microsatellites reported here allows these markers to be a useful resource for genetic studies of S. acapulcoensis and some of their congeners to address evolutionary, ecological and conservation-related questions in the Eastern Tropical Pacific.
KeywordsStegastes Marker isolation Microsatellites Cross-amplification Population genetics Eastern Pacific endemics
This project was supported with funds from COLCIENCIAS (Colombian Administrative Department of Science, Technology and Innovation) grant No. FP44842-540-2014 awarded to FAZ and the Coral Reef Ecology Research Group of Universidad del Valle, Colombia. We are grateful for the technical support provided by the Human Genetic Lab and the Ichthyology Lab of Universidad del Valle, and the Molecular Ecology and Evolution Laboratory (MEEL) of James Cook University, Townsville, Australia. Thanks also to Floriaan Devlo-Delva for his assistance in the lab. We thank the System of National Natural Parks of Colombia and Malpelo and other Marine Ecosystems Foundation for logistical support in the field. We also thank the Marine and Coastal Research institute of Colombia, INVEMAR, for their support within the academic and scientific cooperation agreement No. 010-12. Microsatellite enrichment was performed in the Pritzker Laboratory for Molecular Systematics and Evolution operated with support from the Pritzker Foundation.
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Conflict of interest
The authors declare that they have no conflict of interest.
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