Marine Biodiversity

, Volume 41, Issue 2, pp 313–323

New records and molecular characterization of Acrozoanthus (Cnidaria: Anthozoa: Hexacorallia) and its endosymbionts (Symbiodinium spp.) from Taiwan

  • James Davis Reimer
  • Sohta A. Ishikawa
  • Mamiko Hirose
Original Paper

DOI: 10.1007/s12526-010-0069-5

Cite this article as:
Davis Reimer, J., Ishikawa, S.A. & Hirose, M. Mar Biodiv (2011) 41: 313. doi:10.1007/s12526-010-0069-5

Abstract

During a recent survey of the zoanthid (Cnidaria: Anthozoa) fauna of Taiwan, specimens resembling Acrozoanthus australiae (family Zoanthidae) were found at Kenting and Green Island, Taiwan, attached to eunicid worm tubes growing out from under large Porites coral colonies in coral reef environments. As this species had previously been described only from eunicid worm tubes in mud flats in Australia and Indonesia, and no studies had specifically examined its phylogenetic position, molecular and morphological examinations were conducted to determine the identity of the Taiwan specimens, and its phylogenetic relationships with other Zoanthidae genera and species. At the same time, endosymbiotic Symbiodinium types within specimens were also investigated. Results from the phylogenetic analyses of sequences of three DNA markers [cytochrome oxidase subunit I, mitochondrial 16S ribosomal DNA, internal transcribed spacer 2 of ribosomal DNA (ITS-2)] strongly suggested that the Taiwan specimens were identical with A. australiae. Based on endosymbiont ITS-2 sequences, these colonies were in symbiosis with Symbiodinium clade D1a (= S. trenchii), theorized to be adapted to both comparatively cold and hot marine environments. Furthermore, phylogenetic analyses from all three zoanthid DNA markers suggest that Acrozoanthus may be within the closely related genus Zoanthus. This study demonstrates the overall lack of data on zoanthid species’ distributions, and it is recommended the diversity of zoanthids within the nearby Coral Triangle be investigated to link Indo-Australian zoanthid data with information from Japan and the northwest Pacific.

Keywords

Acrozoanthus Symbiodinium Zoanthid COI mt 16S rDNA ITS-rDNA 

Supplementary material

12526_2010_69_Fig4_ESM.gif (116 kb)
Fig. S1

ML tree of the ITS-rDNA sequences for clade D Symbiodinium including specimens from this study. Values at branches represent ML and NJ bootstrap probabilities, respectively. New isolates and sequences from this study in bold (GIF 116 kb)

12526_2010_69_MOESM1_ESM.eps (310 kb)
High resolution image (EPS 309 kb)
12526_2010_69_MOESM2_ESM.xls (7 kb)
Table S1(XLS 7 kb)

Copyright information

© Senckenberg, Gesellschaft für Naturforschung and Springer 2010

Authors and Affiliations

  • James Davis Reimer
    • 1
    • 2
  • Sohta A. Ishikawa
    • 3
  • Mamiko Hirose
    • 1
  1. 1.Molecular Invertebrate Systematics and Ecology Laboratory, Rising Star Program, Transdisciplinary Research Organization for Subtropical Island Studies (TRO-SIS)University of the RyukyusNishiharaJapan
  2. 2.Marine Biodiversity Research ProgramInstitute of Biogeosciences, Japan Agency for Marine-Earth Science and Technology (JAMSTEC)YokosukaJapan
  3. 3.Graduate School of Life and Environmental SciencesUniversity of TsukubaTsukubaJapan

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