Marine Biodiversity

, Volume 40, Issue 4, pp 237–247

Preliminary analyses of cultured Symbiodinium isolated from sand in the oceanic Ogasawara Islands, Japan

  • James Davis Reimer
  • Md Mahfuzur Rahman Shah
  • Frederic Sinniger
  • Kensuke Yanagi
  • Shoichiro Suda
Original paper

DOI: 10.1007/s12526-010-0044-1

Cite this article as:
Reimer, J.D., Shah, M.M.R., Sinniger, F. et al. Mar Biodiv (2010) 40: 237. doi:10.1007/s12526-010-0044-1

Abstract

The dinoflagellate genus Symbiodinium is generally found in many tropical and subtropical marine invertebrates. Recently, reports have focused on free-living types. We examined free-living Symbiodinium from the Ogasawara (Bonin) Islands, a group of oceanic islands south of Japan. Examining sand samples, seven of eight initial isolates were successfully cultured. Genetic analyses of 18S, 28S and internal transcribed spacer (ITS) ribosomal DNA regions reveal that one isolate cultured with only IMK was identical to clade A isolated from coral reef sand in Okinawa, and four additional isolates cultured with only IMK comprised a new clade A lineage. Additionally, two isolates cultured with IMK and soil extract were closely related to a little-known divergent lineage within clade D. Our results demonstrate some free-living Symbiodinium types may have very wide distributions, and that utilizing different culturing techniques will further discovery of unique Symbiodinium lineages from environmental samples.

Keywords

Zooxanthellae Symbiodinium Free-living Ogasawara (Bonin) Islands Sand 

Supplementary material

12526_2010_44_Fig4_ESM.jpg (54 kb)
Fig. S1

ML tree of 28S-rDNA sequences for Symbiodinium, including specimens from this study. Values at branches represent ML and NJ bootstrap probabilities, respectively. Monophylies with more than 95% Bayesian posterior probabilities are shown by thick branches. New isolates and sequences from this study in bold. Sequences used in the alignment for this tree are in Table 1 (JPEG 54 kb)

12526_2010_44_MOESM1_ESM.eps (338 kb)
High resolution image. (EPS 338 kb)
12526_2010_44_Fig5_ESM.jpg (58 kb)
Fig. S2

ML tree of the ITS-rDNA sequences for Symbiodinium, including specimens from this study. Values at branches represent ML and NJ bootstrap probabilities, respectively. Monophylies with more than 95% Bayesian posterior probabilities are shown by thick branches. New isolates and sequences from this study in bold. Sequences used in the alignment for this tree are in Table 1 (JPEG 58 kb)

12526_2010_44_MOESM2_ESM.eps (375 kb)
High resolution image (EPS 374 kb)

Copyright information

© Senckenberg, Gesellschaft für Naturforschung and Springer 2010

Authors and Affiliations

  • James Davis Reimer
    • 1
    • 2
  • Md Mahfuzur Rahman Shah
    • 3
  • Frederic Sinniger
    • 4
    • 5
  • Kensuke Yanagi
    • 6
    • 7
  • Shoichiro Suda
    • 5
  1. 1.Molecular Invertebrate Systematics and Ecology Laboratory, Rising Star Program, Trans-disciplinary Organization for Subtropical Island StudiesUniversity of the RyukyusOkinawaJapan
  2. 2.Marine Biodiversity Research Program, Institute of BiogeosciencesJapan Agency for Marine-Earth Science and Technology (JAMSTEC)KanagawaJapan
  3. 3.Graduate Shool of Engineering and ScienceUniversity of the RyukyusOkinawaJapan
  4. 4.Department of OceanographyFlorida State UniversityTallahasseeUSA
  5. 5.Department of Chemistry, Biology and Marine Science, Faculty of ScienceUniversity of the RyukyusOkinawaJapan
  6. 6.Chiba Biodiversity CenterChibaJapan
  7. 7.Natural History Museum and Institute ChibaChibaJapan

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