Reproductive Medicine and Biology

, Volume 11, Issue 3, pp 129–133 | Cite as

Combination of recombinant follicle stimulating hormone with human menopausal gonadotrophin or recombinant luteinizing hormone in a long gonadotrophin-releasing hormone agonist protocol: a retrospective study

  • Gottumukkala Achyuta Rama Raju
  • S. C. Teng
  • Prathigudupu Kavitha
  • Balabomma Kavitha Lakshmi
  • Cheemakurthi Ravikrishna
Original Article



To assess the effect of supplementation with recombinant human luteinizing hormone (rhLH) for patients treated either with recombinant follicle stimulating hormone (rFSH) plus rhLH or with rFSH plus human menopausal gonadotrophin (HMG) in a long gonadotrophin-releasing hormone (GnRH) agonist-stimulation protocol.


A single-centre, retrospective analysis of patients with hypo responsiveness to a long GnRH agonist protocol (n = 174), with consecutive in-vitro fertilization or intracytoplasmic sperm injection cycles, compared the outcomes of long luteal GnRH agonist ovarian stimulation using rFSH combined with HMG (n = 100) versus rFSH combined with rhLH (n = 74). The endpoints included clinical pregnancy, number of oocytes retrieved, and total gonadotrophin dose.


Significantly more clinical pregnancies were achieved after stimulation with rFSH and rhLH than after stimulation with rFSH and HMG (35.1 vs. 19%, p < 0.01). More oocytes were recovered (13.1 vs. 11.3, p = 0.024) with less FSH utilized in the rFSH and rhLH group than in the rFSH and HMG group (2706.4 vs. 4134.2 U, p < 0.001).


Use of rFSH combined with rhLH in long GnRH agonist assisted reproductive technology (ART) cycles was associated with more clinical pregnancies, recovery of more oocytes, and reduction in gonadotrophin use, suggesting that the superior purity and consistency of rFSH and rhLH may result in better clinical outcomes.


ICSI IVF LH Ovarian stimulation Recombinant FSH 



We would like to thank Dr K. Murali Krishna for his help in the preparation of this manuscript.

Conflict of interest

G.A. Ramaraju, Kavitha, Kavitha Lakshmi and Ravikrishna have no conflicts of interest to declare. S.C Teng is an employee of Merck Pte Ltd.


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Copyright information

© Japan Society for Reproductive Medicine 2012

Authors and Affiliations

  • Gottumukkala Achyuta Rama Raju
    • 1
  • S. C. Teng
    • 2
  • Prathigudupu Kavitha
    • 1
  • Balabomma Kavitha Lakshmi
    • 1
  • Cheemakurthi Ravikrishna
    • 1
  1. 1.Krishna IVF ClinicVisakhapatnamIndia
  2. 2.Merck Pte LtdSingaporeSingapore

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