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Dual DNA-protein extraction from human archeological remains

  • Ioana Rusu
  • Ioana Paica
  • Adriana Vulpoi
  • Claudia Radu
  • Cristina Mircea
  • Cătălin Dobrinescu
  • Vitalie Bodolică
  • Beatrice Kelemen
Original Paper
  • 171 Downloads

Abstract

Human skeletal remains may be considered bio-archives due to their particular combination of mechanical, structural, and chemical properties that render natural resistance against post-mortem degradation of ancient biomolecules. The survival of organic matter in ancient tissues is of great interest due to its potential in recovering information on the historical past of individuals and populations including health, dietary history, ancestry, and migration patterns. Even though the ancient biomolecules have become a powerful research tool, the archeogenetic research field can often deal with obstacles regarding the limited availability of osteological material and/or ethical concerns as their analysis is destructive. Here, we describe an alternative, efficient method for simultaneous extraction of DNA and proteins from the same archeological sample source, thus limiting the damage to valuable ancient human skeletal remains. The proposed method consists of overnight decalcification of powdered tissue, purification and concentration of biomolecules, and separation of the resulting solution for subsequent DNA purification and protein precipitation. Its performance was assessed relative to previously described approaches, comparing the concentration of the retrieved biomolecules. Our results show that this method designed to co-purify DNA and proteins is appropriate to obtain authentic sequences of the human mitochondrial control region from medieval or more recent human teeth. Also, the resulting proteins can be used for immunochromatographic detection of malaria in skeletal material suspect of infection with Plasmodium sp.

Keywords

Ancient molecules DNA extraction Mitochondrial DNA Protein extraction Fourier transform InfraRed spectroscopy Malaria diagnosis 

Notes

Funding information

This work was supported by the Executive Unit for Financing Higher Education, Research, Development and Innovation (UEFISCDI), project number PN-II-PT-PCCA-2011-3.1-1153.

Supplementary material

12520_2018_760_MOESM1_ESM.xlsx (13 kb)
ESM 1 (XLSX 13 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Molecular Biology Center, Interdisciplinary Research Institute on Bio-Nano-SciencesBabeș-Bolyai UniversityCluj-NapocaRomania
  2. 2.Department of Molecular Biology and Biotechnology, Faculty of Biology and GeologyBabeș-Bolyai UniversityCluj-NapocaRomania
  3. 3.Institute of Biology Bucharest of Romanian AcademyBucharestRomania
  4. 4.Nanostructured Materials and Bio-Nano-Interfaces Center, Interdisciplinary Research Institute on Bio-Nano-SciencesBabeș-Bolyai UniversityCluj-NapocaRomania
  5. 5.Faculty of History and PhilosophyBabeș-Bolyai UniversityCluj-NapocaRomania
  6. 6.Museum of National History and ArcheologyConstanțaRomania

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