Early Neolithic funerary diversity and mitochondrial variability of two Iberian sites

Original Paper

Abstract

An analysis of the burial characteristics of the individuals recovered from two Early Neolithic sites in Navarre (Los Cascajos and Paternanbidea), in the Spanish Basque Country, revealed a complex funerary ritual. The individuals recovered from the Paternanbidea site were distributed in three double graves and a multiple one, while the individuals from Los Cascajos were buried in individual pit-shaped tombs; furthermore, the tombs had a variety of cultural and funerary features. The aim of this study is to evaluate the burial ritual by means of mitochondrial DNA data and the funerary characteristics of 36 individuals recovered from these two sites. The results show that the diversity of these Early Neolithic burial practices from the northern Iberian Peninsula cannot be explained by means of maternal kinship relationships.

Keywords

Farmer groups Ancient DNA Mitochondrial DNA Funerary diversity Early Neolithic 

References

  1. Achilli A, Rengo C, Magri C, Battaglia V, Olivieri A, Scozzari R, Cruciani F, Zeviani M, Briem E, Carelli V, Moral P, Dugoujon JM, Roostalu U, Loogväli EL, Kivisild T, Bandelt HJ, Richards MR, Villems AS, Santachiara-Benerecetti SO, Torroni A (2004) The molecular dissection of mtDNA haplogroup H confirms that the Franco-Cantabrian glacial refuge was a major source for the European gene pool. Am J Hum 75:910–918CrossRefGoogle Scholar
  2. Alonso A, Albarran C, Martin P, Garcia P, Garcia O, de la Rúa C, Alzualde A, Fernandez De Simon L, Sancho M, Fernandez-Piqueras J (2003) Multiplex-PCR of short amplicons for mtDNA sequencing from ancient DNA. Int Congr Ser 1239:585–588CrossRefGoogle Scholar
  3. Alzualde A, Izagirre N, Alonso S, Alonso A, de la Rúa C (2005) Temporal mitochondrial DNA variation in the Basque Country: influence of post-neolithic events. Ann Hum Genet 69:665–679CrossRefGoogle Scholar
  4. Alzualde A, Izagirre N, Alonso S, Alonso A, Albarran C, Azkarate A, de la Rúa C (2006) Insights into the “isolation” of the Basques: mtDNA lineages from the historical site of Aldaieta (6th-7th centuries AD). Am J Phys Anthropol 130:394–404CrossRefGoogle Scholar
  5. Alzualde A, Izagirre N, Alonso S, Rivera N, Alonso A, Azkarate A, de la Rua C (2007) Influences of the European kingdoms of late antiquity on the Basque country: an ancient DNA study. Curr Anthropol 48:155–162CrossRefGoogle Scholar
  6. Anderson MJ (2003) PCO: a front ran computer program for principal coordinate analysis. Department of Statistics, University of Auckland, New ZealandGoogle Scholar
  7. Andrews RM, Kubacka I, Chinnery PF, Lightowlers RN, Turnbull DM (1999) Reanalysis and revision of the Cambridge reference sequence for human mitochondrial DNA. Nat Genet 23:147CrossRefGoogle Scholar
  8. Ennafaa H, Cabrera VM, Abu-Amero KK, Gonzalez AM, Amor MB, Bouhaha R, Dzimiri N, Elgaaied AB, Larruga JM (2009) Mitochondrial DNA haplogroup H structure in North Africa. BMC Genet 10:8CrossRefGoogle Scholar
  9. Gamba C, Fernández E, Tirado M, Deguilloux MF, Pemonge MH, Utrilla P, Edo M, Molist M, Rasteiro R, Chikhi L, Arroyo-Pardo E (2012) Ancient DNA from an early neolithic Iberian population supports a pioneer colonization by first farmers. Mol Ecol 21:45–56CrossRefGoogle Scholar
  10. García-Gazólaz J, Sesma J (2007a) Los enterramientos en el poblado Neolitico de Los Cascajos (Los Arcos) In La tierra te sea leve Arqueología de la muerte en Navarra 49–58 Navarra: Pamplona Hispania DeorumGoogle Scholar
  11. García-Gazólaz J, Sesma J (2007b) Los enterramientos neolíticos del yacimiento de Paternanbidea (Ibero) In La tierra te sea leve Arqueología de la muerte en Navarra: 59–65. Pamplona Hispania Deorum, NavarraGoogle Scholar
  12. Gilbert MT, Hofreiter M, Barnes I (2005) Assessing ancient DNA studies. Trends Ecol Evol 20:541–544CrossRefGoogle Scholar
  13. Hervella M, Izagirre N, Alonso S, Fregel R, Alonso A, Cabrera VM, de la Rúa C (2012) Ancient DNA from hunter-gatherer and farmer groups from Northern Spain supports a random dispersion model for the Neolithic expansion into Europe. Plos One 7:e34417CrossRefGoogle Scholar
  14. Izagirre N, de la Rúa C (1999) An mtDNA analysis in ancient Basque populations: implications for haplogroup V as a marker for a major Palaeolithic expansion from southwestern Europe. Am J Hum Genet 65:199–207CrossRefGoogle Scholar
  15. Lacan M, Keyser C, Ricaut FX, Brucato N, Tarrús J, Bosch A, Guilaine J, Crubézy E, Judes B (2011) Ancient DNA suggests the leading role played by men in the Neolithic dissemination. Proc Natl Acad Sci U S A 108:18255–18259CrossRefGoogle Scholar
  16. Lee EJ, Makarewicz C, Renneberg RM, Harder B, Krause-Kyoramüller Ostritz S, Fehren-Schmitz L, Schreiber S, Müller J, Von Wurmb-Schwark N, Nebel A (2012) Emerging genetic patterns of the European neolithic: perspectives from a late neolithic bell beak burial site in Germany. Am J Phys Anthropol 148:571–779CrossRefGoogle Scholar
  17. Nei M (1987) Molecular Evolutionary Genetics New York: Columbia University PressGoogle Scholar
  18. Pääbo S, Poinar H, Serre D, Jaenicke-Despres V, Hebler J, Rohland N, Kuch J, Krause M, Vigilant L, Hofreiter M (2004) Genetic analyses from ancient DNA. Ann Rev Genet 38:645–679CrossRefGoogle Scholar
  19. Peña-Chocarro L, Zapata L, Iriarte MJ, González-Morales M, Straus LG (2005) The oldest agriculture in northern Atlantic Spain: new evidence from El Miron Cave (Ramales de la Victoria Cantabria). J Archaeol Sci 32:579–587CrossRefGoogle Scholar
  20. Raymond M, Rousset F (1995) An exact test for population differentiation. J Evol 49:1280–1283CrossRefGoogle Scholar
  21. Sampietro ML, Lao O, Caramelli ML, Pou R, Marti M, Bertrenpetit J, Lalueza-Fox C (2007) Palaeogenetic evidence supports a dual model of Neolithic spreading into Europe. Proc R Soc B 274:2161–2167CrossRefGoogle Scholar
  22. Schneider S, Excoffier L (1999) Estimation of past demographic parameters from the distribution of pairwise differences when the mutation rates vary among sites: application to human mitochondrial DNA. Genetics 152:1079–1089Google Scholar
  23. Soares P, Achilli A, Semino O, Davies W, Macaulay V, Bandelt HJ, Torroni A, Richards MB (2010) The archaeogenetics of Europe. Curr Biol 20:R174–R183CrossRefGoogle Scholar
  24. Torroni A, Huoponen K, Francalacci P, Petrozzi M, Morelli L, Scozzari R, Obinu D, Savontaus ML, Wallace DC (1996) Classification of European mtDNAs from an analysis of three European populations. Genetics 144:1835–1850Google Scholar
  25. White TD, Folkens PA (2005) The Human Bone Manual Elsevier Academic PressGoogle Scholar
  26. Zapata L, Peña-Chocarro L, Perez-Jordá G, Stika HP (2004) Early neolithic agriculture in the iberian peninsula. J World Prehist 18:283–325CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  1. 1.Department of Genetics, Physical Anthropology and Animal PhysiologyUniversity of the Basque Country UPV/EHULeioaSpain
  2. 2.Department of GeneticsUniversity of La LagunaLa LagunaSpain

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