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World Journal of Pediatrics

, Volume 9, Issue 3, pp 245–250 | Cite as

The differences in T and B cell subsets in thyroid of children with Graves’ disease and Hashimoto’s thyroiditis

  • Iwona Ben-SkowronekEmail author
  • Leszek Szewczyk
  • Beata Kulik-Rechberger
  • Elzbieta Korobowicz
Original Article

Abstract

Background

The differences between Graves’ disease (GD) and Hashimoto’s thyroiditis (HT) suggest that changes in the subsets of T cells may have an influence on the course of these reactions.

Methods

This study included 90 children: 30 with GD, 30 with HT, and 30 healthy children as controls. After thyroidectomy, standard histological examinations and immunohistochemical reactions were performed in paraffin specimens with monoclonal antibodies against T cell markers CD3, CD4, CD8 as well as against CD79 alpha B cells. Ultrathin sections were examined under a transmission electron microscope.

Results

Autoimmune reaction in GD consisted of an increased number of CD4+ T cells (3.17±4.27%) and plasma cells (22.89±8.61%) producing thyroidstimulating hormone-receptors and stimulating thyrocytes to activity. The number of CD8+ T cells was increased in children with HT (20.54±0.68%) as compared with the controls (0.65±0.30%). The autoimmune reaction in the HT children showed antibody dependent cytotoxicity with a low number of CD4+ T cells and an increased number of CD8+ T cells in the thyroid tissue in comparison with that in the GD children and the controls. Plasma cells (31.65±9.11%) in this situation produced the antibodies involved in cytotoxic reactions against thyrocytes.

Conclusions

Graves’ disease is characterized by the increased number of CD4+ T cells and CD8+ T cells. Hashimoto’s thyroiditis is characterized by the low number of CD4+ T cells and increased number of CD8+ T cells. CD8+ T cells have cytotoxic properties only in Hashimoto’s thyroiditis.

Key words

autoimmunity lymphocytes thyroid disorders 

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Copyright information

© Children's Hospital, Zhejiang University School of Medicine and Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Iwona Ben-Skowronek
    • 1
    Email author
  • Leszek Szewczyk
    • 1
  • Beata Kulik-Rechberger
    • 2
  • Elzbieta Korobowicz
    • 3
  1. 1.Department of Pediatric Endocrinology and DiabetologyMedical UniversityLublinPoland
  2. 2.Department of Neonatology and Infant PathologyMedical UniversityLublinPoland
  3. 3.Department of PathologyMedical UniversityLublinPoland

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