Fractionation of heavy metals in contaminated soil after amendment with composted cow manure and poultry litter

  • Bushra Haroon
  • Muhammad IrshadEmail author
  • Farhan HafeezEmail author
  • Arshid Pervez
  • Faridullah
Part of the following topical collections:
  1. Implications of Biochar Application to Soil Environment under Arid Conditions


Irrigation of agricultural fields with industrial wastewater results in the accumulation of heavy metals (HM) in soils. This situation could be harmful to animals and humans health. Therefore, this study investigated HM fractions in soils treated with composted cow manure and poultry litter. Before application, manures were composted with privet and Italian cypress residues at the ratio of 1:0, 1:1, and 1:2 (animal manure:plant residues). The results showed that extraction of HM fractions reduced from contaminated soils depending upon the type and ratio of composted manure applied. Heavy metal fractions in soils reduced significantly as compost application rates increased and decreased as the amount of privet and cypress wastes in the compost increased. Heavy metals were released less from the soil amended with composted poultry litter as compared to soil samples containing cow manure. Mixing of soil with manure containing cypress residues reduced heavy metal concentrations more than the composted material containing privet residues. Concentrations of HM in polluted soil varied as Pb > Cu > Zn > Ni > Cd irrespective of the extracting agent. The concentration of HM differed among fractions as residual metal > oxide associated metal > carbonate associated metal > organically bound metal > exchangeable metal. Overall treatments of manures composted with plant waste reduced the extractability of HM from soil, and this would be beneficial for limiting HM bioavailability from contaminated soils.


Fractionation Heavy metals Contaminated soil Cow manure Poultry litter Privet Cypress 


Funding information

This research work received funding through national research program for universities (project no. NRPU-4831) by the Higher Education Commission of Pakistan.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


  1. Abbas T, Rizwan M, Ali S, Zia-ur-Rehman M, Qayyum MF, Abbas F, Ok YS (2017) Effect of biochar on cadmium bioavailability and uptake in wheat (Triticum aestivum L.) grown in a soil with aged contamination. Ecotoxicol Environ Saf 140:37–47CrossRefGoogle Scholar
  2. Abdel-Salam AA, Salem HM, Abdel-Salam MA, Seleiman MF (2015) Phytochemical removal of heavy metal-contaminated soils. Heavy metal contamination of soils 44: 299–309Google Scholar
  3. Adriano DC, Wenzel WW, Vangronsveld J, Bolan NS (2004) Role of assisted natural remediation in environmental cleanup. Geoderma 122:121–142CrossRefGoogle Scholar
  4. Alamgir M, Marschner P (2016) Changes in P pools over three months in two soils amended with legume residues. J Soil Sci Plant Nutr 16:76–87Google Scholar
  5. Ali S, Rizwan M, Bano R, Bharwana SA, Rehman MZ, Hussain MB, Al-Wabel MI (2018) Effects of biochar on growth, photosynthesis and chromium (Cr) uptake in Brassica rapa L. under Cr stress. Arab J Geosci 11:1–9CrossRefGoogle Scholar
  6. Alwaneen WS (2016) Cow manure composting by microbial treatment for using as potting material: an overview. Pak J Biol Sci 19(1):1–10CrossRefGoogle Scholar
  7. Amacher MC (1996) Nickel, cadmium, and lead. In Methods of soil analysis, part 3: chemical methods; Spark DL, Ed.; SSSA Book Series No 5, SSSA and ASA, Madison. 739–768Google Scholar
  8. Anwar S, Nawaz MF, Gul S, Rizwan M, Ali S, Kareem A (2016) Uptake and distribution of minerals and heavy metals in commonly grown leafy vegetable species irrigated with sewage water. Environ Monit Assess 188:541CrossRefGoogle Scholar
  9. Asante K (2017) Public health risk assessment for human exposure to chemicals, vol 27. SpringerGoogle Scholar
  10. Balkhair KS, Ashraf MA (2016) Field accumulation risks of heavy metals in soil and vegetable crop irrigated with sewage water in western region of Saudi Arabia. Saudi J Biol Sci 23(1):S32–S44CrossRefGoogle Scholar
  11. Basta NT, Ryan JA, Chaney RL (2005) Trace element chemistry in residual-treated soil: key concepts and metal bioavailability. J Environ Qual 34:49–63CrossRefGoogle Scholar
  12. Brown S, Chaney RL, Hallfrisch JG, Xue Q (2003) Effect of biosolids processing on lead bioavailability in an urban soil. J Environ Qual 32(1):100–108CrossRefGoogle Scholar
  13. Bushra H, Abbasi AM, Faridullah PA, Pervez A, Irshad M (2018) Chemical characterization of cow manure and poultry manure after composting with privet and cypress residues. Commun Soil Sci Plant Anal 49(22):2854–2866CrossRefGoogle Scholar
  14. Calmon C (2018) Ion exchange pollution control: volume II. CRC PressGoogle Scholar
  15. Charles A, Rochette P, Whalen JK, Angers DA, Chantigny MH, Bertrand N (2017) Global nitrous oxide emission factors from agricultural soils after addition of organic amendments: a meta-analysis. Agric Ecosyst Environ 236:88–98CrossRefGoogle Scholar
  16. Chatterjee D, Datta SC, Manjaiah KM (2014) Fractions, uptake and fixation capacity of phosphorus and potassium in three contrasting soil orders. J Soil Sci Plant Nutr 14:640–656Google Scholar
  17. Clemente R, Bernal MP (2006) Fractionation of heavy metals and distribution of organic carbon in two contaminated soils amended with humic acids. Chemosphere 64:1264–1273CrossRefGoogle Scholar
  18. De la Fuente C, Clemente R, Martinez-Alcala I, Tortosa G, Pilar Bernal M (2011) Impact of fresh and composted solid olive husk and their water-soluble fractions on soil heavy metal fractionation; microbial biomass and plant uptake. J Hazard Mater 186(2–3):1283–1289CrossRefGoogle Scholar
  19. Dikinya O, Mufwanzala N (2010) Chicken manure-enhanced soil fertility and productivity: effects of application rates. J Soil Sci Environ Manage 1(3):46–54Google Scholar
  20. Dudka S, Piotrowska M, Chlopecka A (1994) Effect of elevated concentrations of Cd and Zn in soil on spring wheat yield and the metal contents of the plants. Water Air Soil Pollut 76(3–4):333–341CrossRefGoogle Scholar
  21. El-Ramady H, Alshaal T, Abowaly M, Abdalla N, Taha HS, Al-Saeedi AH, Sztrik A (2017) Nanoremediation for sustainable crop production. In: Nanoscience in food and agriculture, vol 5, pp 335–363CrossRefGoogle Scholar
  22. Gee GW, Bauder JW (1986) Particle-size analysis. Methods of soil analysis: part 1. Physical and mineralogical methods 383–411Google Scholar
  23. Gul S, Naz A, Khan A, Nisa S, Irshad M (2016) Phytoavailability and leachability of heavy metals from contaminated soil treated with composted livestock manure. Soil Sed Contam Inter J 25(2):181–194CrossRefGoogle Scholar
  24. Gupta AK, Sinha S (2007) Phytoextraction capacity of the plants growing on tannery sludge dumping sites. Bioresour Technol 98(9):1788–1794CrossRefGoogle Scholar
  25. Hafeez F, Rizwan M, Saqib M, Yasmeen T, Ali S, Abbas T, Rehman MZ, Qayyum MF (2019) Residual effect of biochar on growth, antioxidant defense and cadmium (Cd) accumulation in rice in a Cd-contaminated saline soil. Pak J Agric Sci 56:197–204Google Scholar
  26. Hnyine ZT, Sagala S, Lubis W, Yamin D (2016) Benefits of rural biogas implementation to economy and environment: Boyolali case study. Forum Geografi 29(2):115–128CrossRefGoogle Scholar
  27. Irshad M, Gul S, Eneji AE, Anwar Z, Ashraf M (2014) Extraction of heavy metals from manure and their bioavailability to spinach (Spinacia oleracea L.) after composting. J Plant Nutr 37(10):1661–1675CrossRefGoogle Scholar
  28. Irshad M, Naureen R, Faridullah (2015) Assessing selected heavy metals in vegetables and soils irrigated with wastewater at Haripur, Pakistan. Minerva Biotecnol 27:99–105Google Scholar
  29. Kargar M, Clark OG, Hendershot WH, Jutras P, Prasher SO (2015) Immobilization of trace metals in contaminated urban soil amended with compost and biochar. Water Air Soil Pollut 226(6):1–12CrossRefGoogle Scholar
  30. Kumpiene J, Lagerkvist A, Maurice C (2008) Stabilization of As, Cr, Cu, Pb, and Zn in soil using amendments: a review. Waste Manag 28:215–225CrossRefGoogle Scholar
  31. Lagomarsino A, Mench M, Marabottini R, Pignataro A, Grego S, Renella G (2011) Copper distribution and hydrolase activities in a contaminated soil amended with dolomitic limestone and compost. Ecotoxicol Environ Saf 74(7):2013–2019CrossRefGoogle Scholar
  32. Liu L, Chen H, Cai P, Liang W, Huang Q (2009) Immobilization and phytotoxicity of Cd in contaminated soil amended with chicken manure compost. J Hazard Mater 163(2–3):563–567CrossRefGoogle Scholar
  33. Liu SH, Zeng GM, Niu QY, Liu Y, Zhou L, Jiang LH, Cheng M (2017) Bioremediation mechanisms of combined pollution of PAHs and heavy metals by bacteria and fungi: a mini review. Bioresour Technol 224:25–33CrossRefGoogle Scholar
  34. Loper S, Shober AL, Wiese C, Denny GC, Stanley CD, Gilman EF (2010) Organic soil amendment and tillage affect soil quality and plant performance in simulated residential landscapes. Hortic Sci 45(10):1522–1528Google Scholar
  35. Ma Y, Rajkumar M, Zhang C, Freitas H (2016) Beneficial role of bacterial endophytes in heavy metal phytoremediation. J Environ Manag 174:14–25CrossRefGoogle Scholar
  36. Madejón E, Madejón P, Burgos P, Pérez de Mora A, Cabrera F (2008) Trace elements, pH and organic matter evolution in contaminated soils under assisted natural remediation: a 4 year field study. J Hazard Mater 162:931–938CrossRefGoogle Scholar
  37. Mahmoud EK, Ghoneim AM (2016) Effect of polluted water on soil and plant contamination by heavy metals in El-Mahla El-Kobra, Egypt. Solid Earth 7(2):703–711CrossRefGoogle Scholar
  38. McLaren RG, Clucas LM (2001) Fractionation of copper, nickel, and zinc in metal-spiked sewage sludge. J Environ Qual 30(6):1968–1975CrossRefGoogle Scholar
  39. Mench M, Lepp N, Bert V, Schwitzguébel JP, Gawronski SW, Schöder J (2010) Vangronsveld, Successes and limitations of phytotechnologies at field scale: outcomes, assessment and outlook from COST Action 859. J Soils Sediments 10:1039–1070CrossRefGoogle Scholar
  40. Nelson, D.W., Sommers, L. (1982) Total carbon, organic carbon and organic matter. Methods of soil analysis. Part 2. Chemical and microbiological properties 539-579Google Scholar
  41. Park JH, Lamb D, Paneerselvam P, Choppala G, Bolan N, Chung JW (2011) Role of organic amendments on enhanced bioremediation of heavy metalloid contaminated soils. J Hazard Mater 185(2–3):549–574CrossRefGoogle Scholar
  42. Rajeswari TR, Sailaja N (2014) Impact of heavy metals on environmental pollution. J Chem Pharm Sci 3:175–181Google Scholar
  43. Rehman MZU, Rizwan M, Ali S, Ok YS, Ishaque W, Saifullah NMF, Akmal F, Waqar M (2017) Remediation of heavy metal contaminated soils by using Solanum nigrum: a review. Ecotoxicol Environ Saf 143:236–248CrossRefGoogle Scholar
  44. Rizwan M, Ali S, Zia Ur Rehman M, Rinklebec J, Tsang DCW, Bashir A, Maqbool A, Tack FMG, OK YS (2018) Cadmium phytoremediation potential of Brassica crop species: a review. Sci Total Environ:631, 1175–632, 1191Google Scholar
  45. Rosazlin A, Fauziah CI, Wan Rasidah AW, Rosenani AB (2010) Leaching of heavy metals (Cu, Mn, Zn, Ni, Pb and As) after six months application of raw and composted recycled paper mill sludge. 19th World Congress of Soil Science, Soil Solutions for a Changing World. Brisbane, AustraliaGoogle Scholar
  46. Roy S, Kashem M (2014) Effects of organic manures in changes of some soil properties at different incubation periods. Open J Soil Sci 4:81–86CrossRefGoogle Scholar
  47. Saha JK, Selladurai R, Coumar MV, Dotaniya ML, Kundu S, Patra AK (2017) Soil pollution-an emerging threat to agriculture. Soil Poll 21(2):43–58Google Scholar
  48. Sarwar N, Imran M, Shaheen MR, Ishaque W, Kamran MA, Matloob A, Hussain S (2017) Phytoremediation strategies for soils contaminated with heavy metals: modifications and future perspectives. Chemosphere 171:710–721CrossRefGoogle Scholar
  49. Scharenbroch BC (2009) A meta-analysis of studies published in arboriculture and urban forestry relating to organic materials and impacts on soil, tree, and environmental properties. J Arboric 35(5):221–231Google Scholar
  50. Shen F, Liao R, Ali A, Mahar A, Guo D, Li R, Zhang Z (2017) Spatial distribution and risk assessment of heavy metals in soil near a Pb/Zn smelter in Feng County, China. Ecotoxicol Environ Saf 139:254–262CrossRefGoogle Scholar
  51. Silveira MLA, Alleoni LRF, Guilherme LRG (2003) Biosolids and heavy metals in soils. Sci Agric 60(4):793–806CrossRefGoogle Scholar
  52. Singh WR, Pankaj SK, Kalamdhad AS (2015) Reduction of bioavailability and leachability of heavy metals during agitated pile composting of Salvinia natans weed of Loktak lake. Int J Recycl Org Waste Agric 4(2):143–156CrossRefGoogle Scholar
  53. Steffan JJ, Brevik EC, Burgess LC, Cerdà A (2018) The effect of soil on human health: an overview. Eur J Soil Sci 69(1):159–171CrossRefGoogle Scholar
  54. Thomas EY, Dauda SO (2015) Comparative effects of compost and poultry manure on bioavailability of Pb and Cu and their uptake by maize. New York Sci J 8:72–81Google Scholar
  55. Tóth G, Hermann T, Da Silva MR, Montanarella L (2016) Heavy metals in agricultural soils of the European Union with implications for food safety. Environ Int 88:299–309CrossRefGoogle Scholar
  56. Udovic M, McBride MB (2012) Influence of compost addition on lead and arsenic bioavailability in reclaimed orchard soil assessed using Porcellioscaber bioaccumulation test. J Hazard Mater 205:144–149CrossRefGoogle Scholar
  57. Walker DJ, Clemente R, Roig A, Bernal MP (2003) The effects of soil amendments on heavy metal bioavailability in two contaminated Mediterranean soils. Environ Pollut 122(2):303–312CrossRefGoogle Scholar
  58. Walker DJ, Clemente R, Bernal MP (2004) Contrasting effects of manure and compost on soil pH, heavy metal availability and growth of Chenopodium album L. in a soil contaminated by pyritic mine waste. Chemosphere 57(3):215–224CrossRefGoogle Scholar
  59. Wolka K, Melaku B (2015) Exploring selected plant nutrient in compost prepared from food waste and cattle manure and its effect on soil properties and maize yield at Wondo Genet, Ethiopia. Environ Sys Res 4(1):9–15CrossRefGoogle Scholar
  60. Wu J, Hou Y, Wen Y (2018) Tourist behavior and conservation awareness on eating wild edible plants in mountainous protected areas: a case study in Northwest China. J Sustain Forest 5:489–503Google Scholar

Copyright information

© Saudi Society for Geosciences 2019

Authors and Affiliations

  1. 1.Department of Environmental SciencesCOMSATS University IslamabadAbbottabadPakistan
  2. 2.Arid Land Research CenterTottori UniversityTottori CityJapan

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