Ecophysiological response of early stage Albizia lebbeck to cadmium toxicity and biochar addition

  • Muhammad Talha Bin Yousaf
  • Muhammad Farrakh Nawaz
  • Hammad Fayyaz Khawaja
  • Sadaf Gul
  • Shafaqat Ali
  • Irfan Ahmad
  • Fahd Rasul
  • Muhammad RizwanEmail author
Part of the following topical collections:
  1. Implications of Biochar Application to Soil Environment under Arid Conditions


Cadmium (Cd) toxicity is among the most prominent issues regarding land degradation. Traditional approaches for soil remediation are not effective. However, phytoremediation is described as the most efficient, economical, and sustainable approach to combat with terrestrial heavy metal pollution. Moreover, if phytoremediation is used in combination with soil amendment, its effectiveness can be increased several folds. Among soil amendments, biochar is considered the best amendment due to its potential for remediation and plant growth. The objective of this study was to determine the phytoremediation potential of Albizia lebbeck in Cd-contaminated and biochar-amended soils. A pot experiment was planned to test the ecophysiological response and Cd uptake of 3-month-old uniform-sized Albizia lebbeck seedlings. Cadmium was induced in soil through irrigational water at the rate of 25 mg/L. Two concentrations of biochar were used: B1 (3%) and B2 (6%). A total of six treatments were applied: Cd0B0 (T1), Cd0B1 (T2), Cd0B2 (T3), Cd1B0 (T4), Cd1B1 (T5), and Cd1B2 (T6), where, Cd0 and B0 designate the control treatment. The plants were harvested after 4 months of growth in the soil with amendments. The maximum growth and gas exchange characteristics were showed by the plant of T3. Cadmium toxicity reduced the growth and gas exchange characteristics to a great extent. Plants of T4 showed the minimum growth compared to the other amendments. The addition of 6% biochar in Cd-contaminated soil (T6) resulted in the decent increase in the growth of plants. The maximum root length (67.18 cm) and shoot length (80.06 cm) were observed in the plants of T3, whereas, the minimum root length (13.94 cm) and shoot length (35.03 cm) were observed in the plants of T4. This study suggests that biochar can be used to enhance the growth of agroforestry tree species like Albizzia lebbek in Cd-contaminated soils.


Heavy metal toxicity Phytoremediation Albizzia lebbek Biochar Agroforestry 



The authors are thankful to University of Agriculture Faisalabad for providing laboratory facilities and the Higher Education Commission for funding support of Mr. Talha Bin Yousaf under the Indigenous Scholarship Program.


  1. Abbas T, Rizwan M, Ali S, Rehman MZ, Qayyum MF, Abbas F, Hannan F, Rinklebe J, Ok YS (2017) Effect of biochar on cadmium bioavailability and uptake in wheat (Triticum aestivum L.) grown in a soil with aged contamination. Ecotoxicol Environ Saf 140:37–47CrossRefGoogle Scholar
  2. Abbas Z, Ali S, Rizwan M, Zaheer IE, Malik A, Riaz MA, Shahid MR, Rehman MZ, Al-Wabel MI (2018) A critical review of mechanisms involved in the adsorption of organic and inorganic contaminants through biochar. Arab J Geosci 11:1–23CrossRefGoogle Scholar
  3. Adrees M, Ali S, Rizwan M, Ibrahim M, Abbas F, Farid M, Zia-ur-Rehman M, Irshad MK, Bharwana SA (2015a) The effect of excess copper on growth and physiology of important food crops: a review. Environ Sci Pollut Res 22:8148–8162CrossRefGoogle Scholar
  4. Adrees M, Ali S, Rizwan M, Rehman MZ, Ibrahim M, Abbas F, Farid M, Qayyum MK, Irshad MK (2015b) Mechanisms of silicon-mediated alleviation of heavy metal toxicity in plants: a review. Ecotoxicol Environ Saf 119:186–197CrossRefGoogle Scholar
  5. Afzal S, Nawaz MF, Siddiqui MT, Aslam Z (2018) Comparative study on water use efficiency between introduced species (Eucalyptus camaldulensis) and indigenous species (Tamarix aphylla) on marginal sandy lands of Noorpur Thal. Pak J Agric Sci 55:1Google Scholar
  6. Ali B, Huang CR, Qi ZY, Ali S, Daud MK, Geng XX, Liu HB, Zhou WJ (2013a) 5-Aminolevulinic acid ameliorates cadmium-induced morphological, biochemical, and ultrastructural changes in seedlings of oilseed rape. Environ Sci Pollut Res 20:7256–7267CrossRefGoogle Scholar
  7. Ali B, Wang B, Ali S, Ghani MA, Hayat MT, Yang C, Xu L, Zhou WJ (2013b) 5-Aminolevulinic acid ameliorates the growth, photosynthetic gas exchange capacity, and ultrastructural changes under cadmium stress in Brassica napus L. J Plant Growth Regul 32:604–614CrossRefGoogle Scholar
  8. Ali B, Gill RA, Yang S, Gill MB, Farooq MA, Liu D, Zhou W (2015) Regulation of cadmium-induced proteomic and metabolic changes by 5-aminolevulinic acid in leaves of Brassica napus L. PLoS One 10:1–23Google Scholar
  9. Ali S, Rizwan M, Qayyum MF, Ok YS, Ibrahim M, Riaz M, Arif MS, Hafeez F, Al-Wabel MI, Shahzad AN (2017) Biochar soil amendment on alleviation of drought and salt stress in plants: a critical review. Environ Sci Pollut Res 24:12700–12712CrossRefGoogle Scholar
  10. Anwar S, Nawaz MF, Gul S, Rizwan M, Ali S, Kareem A (2016) Uptake and distribution of minerals and heavy metals in commonly grown leafy vegetable species irrigated with sewage water. Environ Monit Assess 188:1–9CrossRefGoogle Scholar
  11. Beiyuan J, Tsang DC, Valix M, Zhang W, Yang X, Ok YS (2017) Selective dissolution followed by EDDS washing of an e-waste contaminated soil: extraction efficiency, fate of residual metals, and impact on soil environment. Chemosphere 166:489–496CrossRefGoogle Scholar
  12. Christianson L, DeVallance D, Faulkner J, Basden T (2017) Scientifically advanced woody media for improved water quality from livestock woodchip heavy-use areas. Front Environ Sci Eng 3:1–9Google Scholar
  13. Coomes OT, Miltner BC (2017) Indigenous charcoal and biochar production: potential for soil improvement under shifting cultivation systems. Land Degrad Dev 28:811–821CrossRefGoogle Scholar
  14. Cui L, Pan G, Li L, Bian R, Liu X, Yan J, Quan G, Ding C, Chen T, Liu Y, Liu Y (2016) Continuous immobilization of cadmium and lead in biochar amended contaminated paddy soil: a five-year field experiment. Ecol Eng 93:1–8CrossRefGoogle Scholar
  15. Dennehy C, Lawlor PG, Jiang Y, Gardiner GE, Xie S, Nghiem LD (2017) Greenhouse gas emissions from different pig manure management techniques: a critical analysis. Front Environ Sci Eng 11:1–11Google Scholar
  16. Drake JA, Carrucan A, Jackson WR, Cavagnaro TR, Patti AF (2015) Biochar application during reforestation alters species present and soil chemistry. Sci Total Environ 514:359–365CrossRefGoogle Scholar
  17. Feng Z, Zhu L (2017) Sorption of phenanthrene to biochar modified by base. Front Environ Sci Eng 12:1–9CrossRefGoogle Scholar
  18. Hediji H, Djebali W, Belkadhi A, Cabasson C, Moing A, Rolin D (2015) Impact of long-term cadmium exposure on mineral content of Solanum lycopersicum plants: consequences on fruit production. S Afr J Bot 97:176–181CrossRefGoogle Scholar
  19. Hou D, Li F (2017) Complexities surrounding China's soil action plan. Land Degrad Dev 28:2315–2320CrossRefGoogle Scholar
  20. Hou D, O'Connor D, Nathanail P, Tian L, Ma Y (2017) Integrated GIS and multivariate statistical analysis for regional scale assessment of heavy metal soil contamination: a critical review. Environ Pollut 231:1188–1200CrossRefGoogle Scholar
  21. Kaur B, Singh B, Kaur N, Singh D (2018) Phytoremediation of cadmium-contaminated soil through multipurpose tree species. Agrofor Syst 92:473–483Google Scholar
  22. Keller C, Rizwan M, Davidian JC, Pokrovsky OS, Bovet N, Chaurand P, Meunier JD (2015) Effect of silicon on wheat seedlings (Triticum turgidum L.) grown in hydroponics and exposed to 0 to 30 μM cu. Planta 241:847–860CrossRefGoogle Scholar
  23. Lin Q, Xu X, Wang L, Chen Q, Fang J, Shen X (2017) The speciation, leachability and bioaccessibility of cu and Zn in animal manure-derived biochar: effect of feedstock and pyrolysis temperature. Front Environ Sci Eng 3:1–12Google Scholar
  24. McHenry M (2011) Soil organic carbon, biochar, and applicable research results fo increasing farm productivity under Australian agricultural conditions. Commun Soil Sci Plant Anal 42:1187–1199CrossRefGoogle Scholar
  25. Moreno-Barriga F, Diaz V, Acosta JA, Munoz MA, Faz A, Zornoza R (2017) Creation of technosols to decrease metal availability in pyritic tailings with addition of biochar and marble waste. Land Degrad Dev 28:1943–1951CrossRefGoogle Scholar
  26. Nawaz MF, Gul S, Tanvir MA, Akhtar J, Chaudary S, Ahmad I (2016) The influence of NaCl-salinity on Pb-phytoaccumulation and growth of river red gum tree (Eucalyptus camaldulensis Dehnh). Turk J Agric For 40:425–432CrossRefGoogle Scholar
  27. Nawaz MF, Yousaf MTB, Yasin G, Gul S, Ahmad I, Abdullah M, Rafay M, Tanvir M, Asif M, Afzal S (2018) Agroforestry status and its role to sequester atmospheric CO2 under semi-arid climatic conditions in Pakistan. Appl Ecol Environ Res 16:645–661CrossRefGoogle Scholar
  28. Paz-Ferreiro J, Plasencia P, Gasco G, Mendez A (2017) Biochar from pyrolysis of deinking paper sludge and its use in the remediation of Zn-polluted soils. Land Degrad Dev 28:355–360CrossRefGoogle Scholar
  29. Qayyum MF, Rehman MZ, Ali S, Rizwan M, Naeem A, Maqsood MA, Khalid H, Rinklebe J, Ok YS (2017) Residual effects of monoammonium phosphate, gypsum and elemental sulfur on cadmium phytoavailability and translocation from soil to wheat in an effluent irrigated field. Chemosphere 174:515–523CrossRefGoogle Scholar
  30. Rafati M, Khorasani N, Moattar F, Shirvany A, Moraghebi F, Hosseinzadeh S (2011) Phytoremediation potential of Populus alba and Morus alba for cadmium, chromium and nickel absorption from polluted soil. Int J Environ Res 5:961–970Google Scholar
  31. Rehman MZ, Rizwan M, Ghafoor A, Naeem A, Ali S, Sabir M, Qayyum MF (2015) Effect of inorganic amendments for in situ stabilization of cadmium in contaminated soils and its phyto-availability to wheat and rice under rotation. Environ Sci Pollut Res 22:16897–16906CrossRefGoogle Scholar
  32. Rehman MZ, Rizwan M, Ali S, OK YS, Ishaque W, Saifullah, Nawaz MF, Akmal F, Waqar M (2017) Remediation of heavy metal contaminated soils by using Solanum nigrum: a review. Ecotoxicol Environ Saf 143:236–248CrossRefGoogle Scholar
  33. Rehman MZ, Rizwan M, Ali S, Naeem A, Yousaf B, Lui G, Azhar M (2018) A field study investigating the potential use of phosphorus combined with organic amendments on cadmium accumulation by wheat and subsequent rice. Arab J Geosci 11:1–10CrossRefGoogle Scholar
  34. Rizwan M, Ali S, Adrees M, Rizvi H, Rehman MZ, Hannan F, Qayyum MF, Hafeez F, OK YS (2016a) Cadmium stress in rice: toxic effects, tolerance mechanisms and management: a critical review. Environ Sci Pollut Res 23:17859–17879CrossRefGoogle Scholar
  35. Rizwan M, Ali S, Abbas T, Rehman MZ, Hannan F, Keller C, Al-Wabel MI, Ok YS (2016b) Cadmium minimization in wheat: a critical review. Ecotoxicol Environ Saf 130:43–53CrossRefGoogle Scholar
  36. Rizwan M, Ali S, Ibrahim M, Farid M, Adrees M, Bharwana SA, Rehman MZ, Qayyum MF, Abbas F (2016c) Mechanisms of biochar-mediated alleviation of toxicity of trace elements in plants: a critical review. Environ Sci Pollut Res 23:2230–2248CrossRefGoogle Scholar
  37. Rizwan M, Ali S, Qayyum MF, Ok YS, Zia-ur-Rehman M, Abbas Z, Hannan F (2017) Use of maize (Zea mays L.) for phytomanagement of Cd-contaminated soils: a critical review. Environ Geochem Health 39:259–277CrossRefGoogle Scholar
  38. Rizwan M, Ali S, Abbas T, Adrees M, Rehman MZ, Ibrahim M, Abbas F, Qayyum MF, Nawaz R (2018) Residual effects of biochar on growth, photosynthesis and cadmium uptake in rice (Oryza sativa L.) under cd stress with different water conditions. J Environ Manag 206:676–683CrossRefGoogle Scholar
  39. Rizwan M, Ali S, Rehman MZ, Maqbool A (2019) A critical review on the effects of zinc at toxic levels of cadmium in plants. Environ Sci Pollut Res.
  40. Saraswat S, Rai JPN (2011) Prospective application of Leucaena leucocephala for phytoextraction of cd and Zn and nitrogen fixation in metal polluted soils. Int J Phytorem 13:271–288CrossRefGoogle Scholar
  41. Shahid M, Khalid S, Abbas G, Shahid N, Nadeem M, Sabir M (2015) Heavy metal stress and crop productivity. Hakeem KR, editor. In: Crop Production and Global Environmental Issues. Springer Int Publishing pp 1–25Google Scholar
  42. Shen Z, Zhang Y, Jin F, McMillan O, Al-Tabbaa A (2017) Qualitative and quantitative characterisation of adsorption mechanisms of lead on four biochars. Sci Total Environ 609:1401–1410CrossRefGoogle Scholar
  43. Singh AK, Pandeya S (2000) Chemical pools of Cd in sludgetreated soils and their contribution to rajmash (Phaseolus vulgaris L.). J Indian Soc Soil Sci 48:544–551Google Scholar
  44. Singh H, Verma A, Kumar M, Sharma R, Gupta R, Kaur M (2017) Phytoremediation: a green technology to clean up the sites with low and moderate level of heavy metals. Austin Biochem 2:1012Google Scholar
  45. Song Y, Hou D, Zhang J, O'Connor D, Li G, Gu Q (2018) Environmental and socio-economic sustainability appraisal of contaminated land remediation strategies: a case study at a mega-site in China. Sci Total Environ 610:391–401CrossRefGoogle Scholar
  46. Stavi I, Lal R (2013) Agroforestry and biochar to offset climate change: a review. Agron Sustain Dev 33:81–96CrossRefGoogle Scholar
  47. Thomas S, Frye S, Gale N, Garmon M, Launchbury R, Machado N, Melamed S, Murray J, Petroff A, Winsborough C (2013) Biochar mitigates negative effects of salt additions on two herbaceous plant species. J Environ Manag 129:62–68CrossRefGoogle Scholar
  48. Wrobel-Tobiszewska A, Boersma M, Sargison J, Close D, Evelyn K, Adams P (2012) Macadamia biochar as a growth stimulus for Eucalyptus nitens forestry nurseries In 5th Joint Australian and New Zealand. Soil Science Conference 518–521Google Scholar
  49. Yang D, Liu Y, Liu S, Huang X, Li Z, Tan X, Zhou L (2017) Potential benefits of biochar in agricultural soils: a review. Pedosphere 27:645–661CrossRefGoogle Scholar
  50. Younis U, Malik SA, Rizwan M, Qayyum MF, Ok YS, Shah MH, Rehman RA, Ahmad N (2016) Biochar enhances the cadmium tolerance in spinach (Spinacia oleracea) through modification of cd uptake and physiological and biochemical attributes. Environ Sci Pollut Res 23:21385–21394CrossRefGoogle Scholar
  51. Zama EF, Zhu YG, Reid BJ, Sun GX (2017) The role of biochar properties in influencing the sorption and desorption of Pb (II), cd (II) and as (III) in aqueous solution. J Clean Prod 148:127–136CrossRefGoogle Scholar

Copyright information

© Saudi Society for Geosciences 2019

Authors and Affiliations

  • Muhammad Talha Bin Yousaf
    • 1
  • Muhammad Farrakh Nawaz
    • 1
  • Hammad Fayyaz Khawaja
    • 1
  • Sadaf Gul
    • 2
  • Shafaqat Ali
    • 3
  • Irfan Ahmad
    • 1
  • Fahd Rasul
    • 4
  • Muhammad Rizwan
    • 3
    Email author
  1. 1.Department of Forestry and Range ManagementUniversity of AgricultureFaisalabadPakistan
  2. 2.Department of BotanyUniversity of KarachiKarachiPakistan
  3. 3.Department of Environmental Sciences and EngineeringGovernment College UniversityFaisalabadPakistan
  4. 4.Department of AgronomyUniversity of AgricultureFaisalabadPakistan

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