Molecular Imaging of Stem Cell Transplantation in Myocardial Disease
Stem cell therapy has been heralded as a novel therapeutic option for cardiovascular disease. In vivo molecular imaging has emerged as an indispensible tool in investigating stem cell biology post-transplantation into the myocardium and in evaluating the therapeutic efficacy. This review highlights the features of each molecular imaging modality and discusses how these modalities have been applied to evaluate stem cell therapy.
KeywordsStem cells Molecular imaging Heart disease
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- 18.• Hendry SL 2nd, van der Bogt KE, Sheikh AY, et al.: Multimodal evaluation of in vivo magnetic resonance imaging of myocardial restoration by mouse embryonic stem cells. J Thorac Cardiovasc Surg 2008, 136:1028–1037; e1021. This study shows significance of transplanted cell viability to get therapeutic benefit.CrossRefPubMedGoogle Scholar
- 24.• Suzuki Y, Zhang S, Kundu P, et al.: In vitro comparison of the biological effects of three transfection methods for magnetically labeling mouse embryonic stem cells with ferumoxides. Magn Reson Med 2007, 57:1173–1179. This study shows SPIO labeling of stem cells does not affect the viability of cells.CrossRefPubMedGoogle Scholar
- 27.• Chen IY, Greve JM, Gheysens O, et al.: Comparison of optical bioluminescence reporter gene and superparamagnetic iron oxide MR contrast agent as cell markers for noninvasive imaging of cardiac cell transplantation. Mol Imaging Biol 2009, 11:178–187. This study demonstrates SPIO direct labeling does not tell viability of transplanted cells.CrossRefPubMedGoogle Scholar
- 30.• Yamada M, Gurney PT, Chung J, et al.: Manganese-guided cellular MRI of human embryonic stem cell and human bone marrow stromal cell viability. Magn Reson Med 2009, 62:1047–1054. This study shows manganese labeling of stem cells does not affect biologic property of human embryonic and mesenchymal stem cells.CrossRefPubMedGoogle Scholar
- 35.• Chung J, Kee K, Rajesh D, et al.: In vivo molecular MRI of embryonic stem cell survival kinetics following transplantation into murine injured myocardium. Circulation 2009, 120:A32. This is the first study to show in vivo viability of embryonic stem cells using an MRI reporter gene.CrossRefGoogle Scholar