Overexpression of A RING finger ubiquitin ligase gene AtATRF1 enhances aluminium tolerance in Arabidopsis thaliana
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Aluminium (Al) toxicity is a primary limitation of crop production in acid soils, which take over 40% of arable soil worldwide. In previous studies, a series of genes have been identified to regulate the plant Al resistance or tolerance. However, none of E3 ubiquitin ligase, the key factor of ubiquitination that plays an important role in plant growth and development, has been characterized for Al response in Arabidopsis. In this study, an E3 ubiquition ligase gene AtATRF1 (Al Tolerance RING Finger 1), a homolog of RAD18 interacting RAD6 to repair the damaged DNA in human and yeast, is isolated from Arabidopsis. It encodes a predicted protein of 296 amino acids with a C3HC4 type RING finger domain. The expression of AtATRF1 is induced by Al, and the transgenic plant overexpressing AtATRF1 enhances the Al tolerance. Similar as RAD18, the AtATRF1 locates in nucleus and regulates the expression of AtATR, which involves in DNA repair and Al response in Arabidopsis. Our results indicate that nuclearlocated AtATRF1 may interact and ubiquitinate the transcriptional regulator of AtATR to mediate the Al tolerance of Arabidopsis.
KeywordsArabidopsis AtATRF1 Al tolerance E3 ubiquitin ligase
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- Bu Q, Li H, Zhao Q, Jiang H, Zhai Q, Zhang J, Wu X, Sun J, Xie Q, Wang D, Li C (2009) The Arabidopsis RING finger E3 ligase RHA2a is a novel positive regulator of abscisic acid signaling during seed germination and early seedling development. Plant Physiol 150:463–481CrossRefPubMedPubMedCentralGoogle Scholar
- Hoekenga OA, Vision TJ, Shaff JE, Monforte AJ, Lee GP, Howell SH, Kochian LV (2003) Identification and characterization of aluminum tolerance loci in Arabidopsis (Landsberg erecta × Columbia) by quantitative trait locus mapping. A physiologically simple but genetically complex trait. Plant Physiol 132:936–948CrossRefPubMedPubMedCentralGoogle Scholar
- Iuchi S, Koyama H, Iuchi A, Kobayashi Y, Kitabayashi S, Ikka T, Hirayama T, Shinozaki K, Kobayashi M (2007) Zinc finger protein STOP1 is critical for proton tolerance in Arabidopsis and coregulates a key gene in aluminum tolerance. Proc Natl Acad Sci USA 104:9900–9905CrossRefPubMedPubMedCentralGoogle Scholar
- Kobayashi Y, Hoekenga OA, Itoh H, Nakashima M, Saito S, Shaff JE, Maron LG, Pineros MA, Kochian LV, Koyama H (2007) Characterization of AtALMT1 expression in aluminum-inducible malate release and its role for rhizotoxic stress tolerance in Arabidopsis. Plant Physiol 145:843–852CrossRefPubMedPubMedCentralGoogle Scholar
- Ma JF (2007) Syndrome of aluminum toxicity and diversity of aluminum resistance in higer plants. Int Rev Cytol 264:226–252Google Scholar
- Yu H, Jiang WZ, Liu Q, Zhang H, Piao MX, Chen ZD, Bian MD (2015) Expression pattern and subcellular localization of the ovate protein family in rice. Plos One 10:69–75Google Scholar
- Zhang H, Shi WL, You JF, Bian MD, Qin XM. Yu H, Liu Q, Peter R, Yang ZM (2015) Transgenic Arabidopsis thaliana plants expressing a β-1,3-glucanase from sweet sorghum (Sorghum bicolor L.) show reduced callose deposition and increased tolerance to aluminium toxicity. Plant Cell Environ 38:1178–1188CrossRefPubMedGoogle Scholar