Advertisement

Journal of Plant Biology

, Volume 59, Issue 2, pp 105–113 | Cite as

Effect of La(NO3)3 and Ce(NO3)3 on shoot induction and seedling growth of in vitro cultured anoectochilus roxburghii

  • Yingya Xu
  • Guifang Zhang
  • Yan Wang
  • Ge Guo
Original Article

Abstract

Anoectochilus roxburghii, a highly valuable medicinal plant species, is threatened in its native habitat. To ensure the sustainability of this useful resource, we studied the effect of La(NO3)3 and Ce(NO3)3 on plant tissue culturepropagated A. roxburghii. Apical buds, mid-stem segments, and basal rhizome segments were taken from aseptic seedlings of A. roxburghii and cultured in vitro on half-strength Murashige-Skoog medium containing different concentrations (up to 5.0 mg/L) of La(NO3)3 or Ce(NO3)3. After 100 d of culturing, average heights of plantlets derived from apical buds were 6.0 cm with 5.0 mg/L La(NO3)3 and 6.2 cm with 2.0 mg/L Ce(NO3)3, which respectively increased by 28.0% and 32.0% as compared with that in the non-treated control group. The optimum concentration for shoot induction from mid-stem segments was 1.0 mg/L Ce(NO3)3 which had a better proliferation times of 1.5-fold and an average length of 3.0 cm compared with 1.0-fold and 2.2 cm in the control group. Optimum growth from basal rhizome segments was achieved on media supplemented with 3.0 mg/L Ce(NO3)3, which provided a better proliferation times of 5.1-fold and an average shoot length of 4.5 cm compared with corresponding control values of 2.0-fold and 3.5 cm. Our results showed that La(NO3)3 and Ce(NO3)3 can accelerate A. roxburghii regeneration, which was probably due to the effect on chlorophyll contents, enzymes activity (superoxide dismutase, catalase and peroxidase) and malonldialedhyde contents caused by the addition of La(NO3)3 or Ce(NO3)3.

Keywords

Anoectochilus roxburghii Cesium nitrate in vitro culture Lanthanum nitrate Plant regeneration 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bowler C, Camp WV, Montagy MV, Inze D (1994) Superoxide dismutase in plants. Crit Rev Plant Sci 13(3):199–218CrossRefGoogle Scholar
  2. Brown PH, Rathjen AH, Graham RD, Tribe DE (1990) In: Gschneidner KA Jr, Eyring L (eds) Handbook on the physics and chemistry of rare earths, vol. 13. Elsevier, Amsterdam, pp 423–453Google Scholar
  3. Burda K, Strzalka K, Schmid, GH (1995) Europium- and dysprosiumions as probes for the study of calcium binding sites in photosystem II. Zeitschrift für Naturforschung 50:220–230Google Scholar
  4. Chen SA, Zhao B, Wang XD, Yuan XF, Wang YH (2003) Promotion of the growth of Crocus sativus cells and the production of crocin by rare earth elements. Biotechnol Lett 26:27–30CrossRefGoogle Scholar
  5. Chen XQ, Stephan WG, Phillip JC, Paul O (2009) Flora of China Editorial Committee, Flora of China, vol. 25 (Orchidaceae). Missouri Botanical Garden Press and Science Press, St. Louis and Beijing, pp 76–80Google Scholar
  6. Diatloff E, Smith FW, Asher CJ (2008) Effects of lanthanum and cerium on the growth and mineral nutrition of corn and mungbean. Ann Bot 101:971–982CrossRefPubMedPubMedCentralGoogle Scholar
  7. Du XM, Irino N, Furusho N, Hayashi J, Shoyama Y (2008) Pharmacologically active compounds in the Anoectochilus and Goodyera species. J Nat Med 62:132–148CrossRefPubMedGoogle Scholar
  8. d’Aquino L, Morgana M, Carboni MA, Staiano M, Antisari MV, Re M, Lorito M, Vinale F, Abadi KM, Woo SL (2009) Effect of some rare earth elements on the growth and lanthanide accumulation in different Trichoderma strains. Soil Biol Biochem 41:2406–2413CrossRefGoogle Scholar
  9. Feng G, Wang XD, Zhao B, Wang YC (2006) Effects of rare earth elements on the growth of Arnebia euchroma cells and the biosynthesis of shikonin. Plant Growth Regul 48:283–290CrossRefGoogle Scholar
  10. Feng WX, Zhang YE, Wang YG, Li FX (1999) The alleviative effects of LaCl3 on the osmotic stress in maize seedlings. Henan Sci 17:45–46Google Scholar
  11. Guo B, Xu LL, Guan ZJ, Wei YH (2012) Effect of lanthanum on rooting of in vitro regenerated shoots of Saussurea involucrata Kar. et Kir. Biol Trace Elem Res 147:334–340CrossRefPubMedGoogle Scholar
  12. Hong FS, Fang N, Gu YH, Zhao GW (1999) Effect of cerium nitrate on seed vigor and activities of enzymes during germination of rice. Chinese Rare Earths 20:45–47Google Scholar
  13. Hong FS, Wang L, Meng XX, Wei Z, Zhao GW (2002a) The effect of cerium (III) on the chlorophyll formation in spinach. Biol Trace Elem Res 89:263–275CrossRefGoogle Scholar
  14. Hong FS, Wei ZG, Zhao GW (2000) Effect of lanthanumon aged seed germination of rice. Biol Trace Elem Res 75:205–213CrossRefGoogle Scholar
  15. Hong FS, Wei ZG, Zhao GW (2002b) Mechanism of lanthanum effect on the chlorophyll of spinach. Sci China Ser C 45:166–176CrossRefGoogle Scholar
  16. Hu Z, Richter H, Sparovek G, Schnug E (2004) Physiological and biochemical effects of rare earth elements on plants and their agricultural significance: a review. J Plant Nutr 27:183–220CrossRefGoogle Scholar
  17. Huang G, Wang L, Zhou Q (2012) Lanthanum (III) regulates the nitrogen assimilation in soybean seedlings under ultraviolet-Bradiation. Biol Trace Elem Res 151:105–112CrossRefPubMedGoogle Scholar
  18. Ippolito MP, Fasciano C, d’Aquino L, Morgana M, Tommasi F (2010) Responses of antioxidant systems after exposition to rare earths and their role in chilling stress in common duckweed (Lemna minor L.): a defensive weapon or a boomerang? Arch Environ Contam Toxicol 58:42–52CrossRefPubMedGoogle Scholar
  19. Ippolito MP, Paciolla C, d’Aquino L, Morgana M, Tommasi F (2007) Effect of rare earth elements on growth and antioxidant metabolism in Lemna minor L. Caryologia 60:125–128CrossRefGoogle Scholar
  20. Jing F, Bei W, Shan XQ, Wang H, Lin J, Zhang S (2007) Evaluation of bioavailability of light rare earth elements to wheat (Triticum aestivum L.) under field conditions. Geoderma 141:53–59CrossRefGoogle Scholar
  21. Liao TJ, Huang Y, Su BY, Zou ZJ (1994) Study of rare earths on yields, qualities and physiological effect of spinach, Rare Earths 15(5): 26–29Google Scholar
  22. Lin CC, Huang PC, Lin JM (2000) Antioxidant and hepatoprotective effects of Anoectochilus formosanus and Gynostemma pentaphyllum. Amer J Clin Med 28:87–96CrossRefGoogle Scholar
  23. Lin JM, Lin CC, Chiu HF, Yang JJ, Lee SG (1993) Evaluation of the anti- inflammatory and liver protective effects of Anoectochilus formosanus, Ganderma lucidum and Gynostemma pentaphyllum. Amer J Clin Med 11:59–69CrossRefGoogle Scholar
  24. Luo AX, Meng ZX, Chen XM, Guo SX (2012) Seed germination and young seedling propagation of Anoectochilus roxburghii. Chin Pharm J 47:1199–1203Google Scholar
  25. Ni JZ (1995) Rare Earth Bioinorganic Chemistry, Science Press, Beijing pp 13–37Google Scholar
  26. Olivares E, Aguiar G, Colonnello G (2011) Rare earth elements in vascular plants: a review. Interciencia 36:331–340Google Scholar
  27. Peng X, Zhou SL, He JY, Ding Li (2013) Influence of rare earth elements on metabolism and related enzyme activity and isozyme expression in Tetrastigma hemsleyanum cell suspension cultures. Biol Trace Elem Res 152:82–90CrossRefGoogle Scholar
  28. Song WP, Hong FS, Wan ZG, Zhou YZ, Gu FG, Xu HG, Yu ML, Chang YH, Zhao MZ, Su JL (2003) Effects of cerium on nitrogen metabolism of peach plantlet in vitro. Biol Trace Elem Res 95:259–268CrossRefGoogle Scholar
  29. Tseng CC, Shang HF, Wang LF, Su B, Hsu CC, Kao HY, Cheng KT (2006) Antitumor and immunostimulating effects of Anoectochilus formosanus Hayata. Phytomedicine 13:366–370CrossRefPubMedGoogle Scholar
  30. Tyler G (2004) Rare earth elements in soil and plant systems—a review. Plant Soil 267:191–206CrossRefGoogle Scholar
  31. Wang BS (1988) Biological free radicals and membrane damage of plants. Plant Physiol Commun 2:12–16Google Scholar
  32. Wang L, Huang X, Zhou Q (2009) Protective effect of rare earth against oxidative stress under ultraviolet-B radiation. Biol Trace Elem Res 128:82–93CrossRefPubMedGoogle Scholar
  33. Wang SY, Kuo YH, Chang HN, Kang PL, Tsay HS, Lin KF, Yang NS, Shyur LF (2002) Profiling and characterization of antioxidant activities in Anoectochilus formosanus Hayata. J Agric Food Chem 50:1859–1865CrossRefPubMedGoogle Scholar
  34. Wang YL, Wang XD, Zhao B, Wang YC (2007) Reduction of hyperhydricity in the culture of Lepidium meyenii shoots by the addition of rare earth elements. Plant Growth Regul 52:151–159CrossRefGoogle Scholar
  35. Wu JY, Wang CG, Mei XG (2001) Stimulation of taxol production and excretion in Taxus spp cell cultures by rare earth chemical lanthanum. J Biotechnol 85:67–73CrossRefPubMedGoogle Scholar
  36. Yang HL, Hu JF, Xu XZ, He JM, Song S (2013) Study on tissue culture and rapid propagation of Anoectochilus roxburghii. Southwest China Journal of Agriculture Science 26:2485–2488Google Scholar
  37. Yuan X, Zhao B, Wang Y (2005) Application of rare earth elements in medicinal plant cell and tissue culture. Chinese Bull Bot 22:115–120Google Scholar
  38. Yuan XF, Wang Q, Zhao B, Wang YC (2002) Improved cell growth and total flavonoids of Saussurea medusa on solid culture medium supplemented with rare earth elements. Biotechnol Lett 24:1889–1892CrossRefGoogle Scholar
  39. Zeng F, Tian HE, Wang Z, Yi A, Gao F, Zhang L, Li F, Shan L (2003) Effect of rare earth element europium on amaranthin synthesis in Amarathus caudatus seedlings. Biol Trace Elem Res 93:271–282CrossRefPubMedGoogle Scholar

Copyright information

© Korean Society of Plant Biologists and Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  1. 1.School of Chinese MedicineGuangzhou University of Chinese MedicineGuangzhou, GuangdongChina

Personalised recommendations