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Journal of Plant Biology

, Volume 59, Issue 2, pp 162–171 | Cite as

Analysis the role of arabidopsis CKRC6/ASA1 in auxin and cytokinin biosynthesis

  • Dong-Wei Di
  • Lei Wu
  • Pan Luo
  • Li Zhang
  • Tian-Zi Zhang
  • Xue Sun
  • Shao-Dong Wei
  • Chen-Wei An
  • Guang-Qin Guo
Original Article

Abstract

The crosstalk between auxin and cytokinin (CK) is important for plant growth and development, although the underlying molecular mechanisms remain unclear. Here, we describe the isolation and characterization of a mutant of Arabidopsis Cytokinin-induced Root Curling 6 (CKRC6), an allele of ANTHRANILATE SYNTHASE ALPHA SUBUNIT 1 (ASA1) that encodes the á-subunit of AS in tryptophan (Trp) biosynthesis. The ckrc6 mutant exhibits root gravitropic defects and insensitivity to both CK and the ethylene precursor 1-aminocyclopropane-1-carboxylicacid (ACC) in primary root growth. These defects can be rescued by exogenous indole-3-acetic acid (IAA) or tryptophan (Trp) supplementation. Furthermore, our results suggest that the ckrc6 mutant has decreased IAA content, differential expression patterns of auxin biosynthesis genes and CK biosynthesis isopentenyl transferase (IPT) genes in comparison to wild type. Collectively, our study shows that auxin controls CK biosynthesis based on that CK sensitivity is altered in most auxin-resistant mutants and that CKs promote auxin biosynthesis but inhibit auxin transport and response. Our results also suggest that CKRC6/ASA1 may be located at an intersection of auxin, CK and ethylene metabolism and/or signaling.

Keywords

Auxin Biosynthesis Cytokinin Ethylene IPTs 

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Copyright information

© Korean Society of Plant Biologists and Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Dong-Wei Di
    • 1
    • 2
  • Lei Wu
    • 1
  • Pan Luo
    • 1
  • Li Zhang
    • 1
  • Tian-Zi Zhang
    • 1
  • Xue Sun
    • 1
  • Shao-Dong Wei
    • 1
  • Chen-Wei An
    • 1
  • Guang-Qin Guo
    • 1
  1. 1.MOE Key Laboratory of Cell Activities and Stress Adaptations, School of Life SciencesLanzhou UniversityLanzhouChina
  2. 2.Institute of Soil ScienceChinese Academy of SciencesNanjingChina

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