Characterization of post-flooding recovery-responsive enzymes in soybean root and hypocotyl
Soybean exhibits markedly reduced growth and yields under flooding stress. To determine the functional roles of four soybean proteins in post-flooding recovery, the organ/stress specificity and time-dependency of their enzymatic activities were analyzed. Peroxidase activity decreased in root and hypocotyl exposed to flooding and cold stresses, but increased during the post-stress recovery period. In contrast, its activity increased in both root and hypocotyl under drought stress. Acid phosphatase activity was suppressed in root treated with flooding and cold stresses, and slightly increased during the recovery period; however, the opposite profile was observed in hypocotyl. In response to drought stress, it did not change in root, but was decreased in hypocotyl. Beta-ketoacyl reductase activity did not change in root under flooding conditions, but was decreased in hypocotyl, although the activity increased slightly during the recovery period. In addition, it was decreased in both organs under drought and cold stresses, but again increased during the recovery period. Nucleotidylyl transferase activity was increased in root under flooding and drought stresses, but was decreased in hypocotyl. It was decreased in response to cold stress, but exhibited a slight increase during the recovery period. Furthermore, the treatment with jasmonate and salicylate suppressed the activities of peroxidase and acid phosphatase in root and hypocotyl under flooding stress; however, the activity of acid phosphatase increased during the recovery period. Nucleotidylyl transferase activity in root was also elevated by treatment with jasmonate, but gradually decreased during the recovery period. These results suggest that jasmonate-induced changes in nucleotidylyl transferase activity may facilitate soybean root recovery after flooding stress.
KeywordsAcid phosphatase Beta-ketoacyl reductase Nucleotidylyl Peroxidase Post-flooding recovery Soybean Transferase
Unable to display preview. Download preview PDF.
- Armstrong W, Drew MC (2002) Root growth and metabolism under oxygen deficiency. In Y Waisel, A Eshel, eds, Plant roots: the hidden half, Ed 3, Marcel Dekker, New York, pp 729–761Google Scholar
- Babu RN, Devaraj VR (2008) High temperature and salt stress response in French bean (Phaseolus vulgaris). Aust J Crop Sci 2:40–48Google Scholar
- Bray EA, Bailey-Serres J, Weretilnyk E (2000) Responses to abiotic stresses. In BB Buchanan, W Gruissem, RL Jones, eds, Biochemistry and molecular biology of plants, American society of plant physiologists, Rockville, Md, pp 1158–1203Google Scholar
- Chen C, Letnik I, Hacham Y, Dobrev P, Ben-Daniel BH, Vankova R, Amir R, Miller G (2014) Ascorbate peroxidase 6 protects Arabidopsis desiccating and germinating seeds from stress and mediates cross talk between reactive oxygen species, abscisic acid, and auxin. Plant Physiol 166:370–383CrossRefPubMedPubMedCentralGoogle Scholar
- Csiszar J, Galle A, Horvath E, Dancso P, Gombos M, Vary Z, Erdei L, Gyorgyey J, Tari I (2012) Different peroxidase activities and expression of abiotic stress-related peroxidases in apical root segments of wheat genotypes with different drought stress tolerance under osmotic stress. Plant Physiol Biochem 52:119–129CrossRefPubMedGoogle Scholar
- Garcion C, Metraux JP (2006) Salicylic acid plant hormone signaling. In P Hedden, SG Thomas, eds, Annual Plant Reviews, Vol 24, Blackwell Press, Oxford, pp 229–257Google Scholar
- Hashida SN, Itami T, Takahasi H, Takahara K, Nagano M, Kawai-Yamada M, Shoji K, Goto F, Yoshihara T, Uchimiya H (2010) Nicotinate/nicotinamide mononucleotide adenyltransferase-mediated regulation of NAD biosynthesis protects guard cells from reactive oxygen species in ABA-mediated stomatal movement in Arabidopsis. J Exp Bot 61:3813–3825CrossRefPubMedGoogle Scholar
- Ishikawa K, Ogawa T, Hirosue E, Nakayama Y, Harada K, Fukusaki E, Yoshimura K, Shigeoka S (2009) Modulation of the poly (ADP-ribosyl) ation reaction via the Arabidopsis ADP-ribose/NADH pyrophosphohydrolase, AtNUDX7, is involved in the response to oxidative stress. Plant Physiol 151:741–54CrossRefPubMedPubMedCentralGoogle Scholar
- Jouili H, Bouazizi H, Ferjani EE (2011) Plant peroxidases: biomarkers of metallic stress Acta Physiol Plant 33:2075–2082Google Scholar
- Komatsu S, Hossain Z (2013) Organ-specific proteome analysis for identification of abiotic stress response mechanism in crop. Front. Plant Sci 14:71Google Scholar
- Nourredine Y, Naima A, Dalila H, Habib S, Karim S, Zohra FLF (2015) Changes of peroxidase activities under cold stress in annuals populations of Medicago. Mol Plant Breed 6:1–9Google Scholar