Growth inhibition and IRT1 induction of Arabidopsis thaliana in response to bismuth
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Abstract
Bismuth (Bi) is used in cosmetics and in semiconductor. And more, Bismuth is used in water pipes as substitute of lead. However, the Bi concentration in soil has not been investigated and no environmental quality standard is available in Japan. Here we investigated the effect of Bi on growth of Arabidopsis thaliana and identified the responsible genes with microarray analysis. The seeds, which were soaked with various concentrations of Bi including agar medium, were not able to germinate by more than 7 µM Bi. The root elongation and shoot growth were significantly inhibited by more than 2 µM Bi. Then, the mRNA was extracted from 2 µM Bi treated seedlings and the gene expression levels were analyzed by microarray. In response to Bi, 13 metal homeostasis genes were expressed 2-fold higher than that of control. The relative transcription level of AtIRT1, the primary Fe2+ uptake transporter in the root, was about 5-fold higher compared to the control. This data is indicating that Bi induces AtIRT1 expression in roots. Moreover, ferritin protein coding genes were one half lesser than that of the control. Bi exposure increased Fe accumulation in roots. These results suggest that Bi might disturb iron homeostasis in seedlings.
Keywords
Arabidopsis thaliana Bismuth Gene expression Iron Iron-Regulated Transporter 1 MicroarrayPreview
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References
- Cataldo DA, Garland TR, Wildung RE (1978) Nickel in plants: I. Uptake kinetics using intact soybean seedlings. Plant Physiol 62:563–565PubMedCentralCrossRefPubMedGoogle Scholar
- Connolly EL, Fett JP, Guerinot ML (2002) Expression of the IRT1 metal transporter is controlled by metals at the levels of transcript and protein accumulation. Plant Cell 14:1347–1357PubMedCentralCrossRefPubMedGoogle Scholar
- Jihou-sha (2011) The Japanese Pharmacopoeia 16th edition (JP16)Google Scholar
- Milner MJ, Seamon J, Craft E, Kochian LV (2013) Transport properties of members of the ZIP family in plants and their role in Zn and Mn homeostasis. J Exp Bot 64:369–381PubMedCentralCrossRefPubMedGoogle Scholar
- Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
- Nagata T, Morita H, Akizawa T, Pan-Hou H (2010) Development of a transgenic tobacco plant for phytoremediation of methylmercury pollution. Appl Microbiol Biotechnol 87:781–786CrossRefPubMedGoogle Scholar
- Nishida S, Aisu A, Mizuno T (2012) Induction of IRT1 by the nickelinduced iron-deficient response in Arabidopsis. Plant Signal Behav 7:329–331PubMedCentralCrossRefPubMedGoogle Scholar
- Nishida S, Tsuzuki C, Kato A, Aisu A, Yoshida J, Mizuno T (2011) AtIRT1, the Primary Iron Uptake Transporter in the Root, Mediates Excess Nickel Accumulation in Arabidopsis thaliana. Plant Cell Physiol 52:1433–1442CrossRefPubMedGoogle Scholar
- Pandey N, Sharma CP (2002) Effect of heavy metals Co2+, Ni2+ and Cd2+ on growth and metabolism of cabbage. Plant Sci 163:753–758CrossRefGoogle Scholar
- Rogers EE, Eide DJ, Guerinot ML (2000) Altered selectivity in an Arabidopsis metal transporter. Proc Natl Acad Sci USA 97:12356–1260PubMedCentralCrossRefPubMedGoogle Scholar
- Sano Y, Satoh H, Chiba M, Okamoto M, Serizawa K, Nakashima H, Omae K (2005a) Oral toxicity of bismuth in rat: single and 28-d repeated administration studies. J Occup Health 47:293–298CrossRefPubMedGoogle Scholar
- Sano Y, Satoh H, Chiba M, Shinohara A, Okamoto M, Serizawa K, Nakashima H, Omae K (2005b) A 13-week toxicity study of bismuth in rats by intratracheal intermittent administration. J Occup Health 47:242–248CrossRefPubMedGoogle Scholar
- Shanmugam V, Lo JC, Wu CL, Wang SL, Lai CC, Connolly EL, Huang JL, Yeh KC (2011) Differential expression and regulation of iron-regulated metal transporters in Arabidopsis halleri and Arabidopsis thaliana-the role in zinc tolerance. New Phytol 190:125–137CrossRefPubMedGoogle Scholar
- Srinarong C, Siramolpiwat S, Wongcha-Um A, Mahachai V, Vilaichone RK (2014) Improved Eradication Rate of Standard Triple Therapy by Adding Bismuth and Probiotic Supplement for Helicobacter pylori Treatment in Thailand. Asian Pac J Cancer Prev 15:9909–9913CrossRefPubMedGoogle Scholar
- Theil EC (1987) Ferritin: structure, gene regulation, and cellular function in animals, plants, and microorganisms. Annu Rev Biochem 56:289–315CrossRefPubMedGoogle Scholar
- Thomas PD, Campbell MJ, Kejariwal A, Mi H, Karlak B, Daverman R, Diemer K, Muruganujan A, Narechania A (2003) PANTHER: a library of protein families and subfamilies indexed by function. Genome Res 13:2129–2141PubMedCentralCrossRefPubMedGoogle Scholar
- Vert G, Barberon M, Zelazny E, Séguéla M, Briat JF, Curie C (2009) Arabidopsis IRT2 cooperates with the high-affinity iron uptake system to maintain iron homeostasis in root epidermal cells. Planta 229:1171–1179CrossRefPubMedGoogle Scholar
- Vert G, Grotz N, Dédaldéchamp F, Gaymard F, Guerinot ML, Briat JF, Curie C (2002) IRT1, an Arabidopsis transporter essential for iron uptake from the soil and for plant growth. Plant Cell 14:1223–1233PubMedCentralCrossRefPubMedGoogle Scholar
- Wilhelmsen I, Weberg R, Berstad K, Hausken T, Hundal O, Berstad A (1994) Helicobacter pylori eradication with bismuth subnitrate, oxytetracycline and metronidazole in patients with peptic ulcer disease. Hepatogastroenterology 41:43–47PubMedGoogle Scholar
- Yang X, Baligar VC, Martens DC, Clark RB (1996) Plant tolerance to nickel toxicity: II. Nickel effects on influx and transport of mineral nutrients four plant species. J Plant Nutr 19:265–279CrossRefGoogle Scholar