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Journal of Plant Biology

, Volume 58, Issue 5, pp 293–302 | Cite as

Knock-down of OsLOX by RNA interference leads to improved seed viability in rice

  • Suyang Bai
  • Niqing He
  • Lu Zhou
  • Beibei Shen
  • Wei Wu
  • Xi Liu
  • Ling JiangEmail author
  • Jianmin Wan
Original Article
  • 194 Downloads

Abstract

Previous work found that lipoxygenases were key enzymes in lipid peroxidation, which causes grain deterioration during storage. In order to obtain better seed viability in rice, 10 marker-free knock-down lines were obtained in the progeny of endogenous OsLOX knock-down mutations caused by the RNAi technology. After artificial accelerated aging, there were four types of knock-down lines with higher seed viability than wild type (receptor parent). OsLOX3 knock-down line NPF1 was of special interest. In a series of experiments, including Southern blots, analysis of OsLOX3 expression, and enzymatic activity, NPF1 had better seed viability than wild-type. We also investigated the main agronomic characters of both knock-down lines and non-transgenetic wild type families. Knock-down lines were identified with generally excellent agronomic characteristics similar to the wild-type.

Keywords

Agronomic characters Enzymatic activity Gene expression Knock-down lines Lipoxygenase Seed viavility 

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Copyright information

© Korean Society of Plant Biologists and Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Suyang Bai
    • 1
  • Niqing He
    • 1
  • Lu Zhou
    • 1
  • Beibei Shen
    • 1
  • Wei Wu
    • 1
  • Xi Liu
    • 1
  • Ling Jiang
    • 1
    Email author
  • Jianmin Wan
    • 1
  1. 1.National Key Laboratory for Crop Genetics & Germplasm Enhancement, Jiangsu Plant Gene Engineering Research CenterNanjing Agricultural University, Research Center of Plant Gene EngineeringNanjing, JiangsuChina

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