Analysis of expressed sequence tags from a normalized cDNA library of perilla (Perilla frutescens)
- 243 Downloads
Perilla (Perilla frutescens), an herbal plant belonging to the Lamiaceae family, has long been cultivated in Asia. Perilla is notable as an aroma-rich leaf vegetable and as the oilseed crop richest in omega-3 fatty acids. However, molecular analysis of this herbal plant is lacking due to insufficient genetic resources. Here, we constructed a normalized cDNA library from whole young perilla plants and analyzed expressed sequence tags (ESTs). A total of 4,582 uniESTs were generated from analysis of 5,435 ESTs. Among these, 307 uniESTs (6.7%) were identified as unique in perilla and 3,625 uniESTs were assigned at least one GO term through similarity searches in public databases. The most frequent GO terms were related to abiotic and biotic stress responses. In addition, we identified 141 uniESTs involved in lipid metabolism, of which four genes encoded fatty acid desaturases. We found one new candidate omega-3 fatty acid desaturase gene, in addition to the four that were previously reported. This analysis of uniESTs from perilla provides a valuable genetic resource to elucidate the molecular underpinnings of lipid metabolism and for molecular breeding of perilla species.
KeywordscDNA library Expressed sequence tag Fatty acid desaturase Perilla
Unable to display preview. Download preview PDF.
- Gwak YH, Kwon JK, Kim SD, Kim HK, Park CB, Bae SB, Bae SD, Oh KO, Yun ES, Lee DC, Lee MH, Jeong CS, Choi YH, Hong YK (2003) Dlggae. Yeongnam Agricultural Research Institute, National Institute of Crop Science (NICS), Rural Development Administration, Republic of KoreaGoogle Scholar
- Ha TH, Lee JH, Lee MH, Lee BW, Kwon HS, Park CH, Shim KB, Kim HT, Baek IY, Jang DS (2012) Isolation and identification of phenolic compounds from the seeds of Perilla frutescens (L.) and their inhibitory activities against a-glucosidase and aldose reductase. Food Chemistry 135:1317–1403CrossRefGoogle Scholar
- Igarashi M, Miyazaki Y (2013) A review on bioactivities of perilla: progress in research on the functions of perilla as medicine and food. Evidence-based complementary and alternative medicine. 2013:Article ID 925342http://dx.doi.org/10.1155/2013/925342Google Scholar
- Li-Beisson Y, Shorrosh B, Beisson F, Andersson MX, Arondel V, Bates PD, Baud S, Bird D, Debono A, Durrett TP, Franke RB, Graham IA, Katayama K, Kelly AA, Larson T, Markham JE, Miquel M, Molina I, Nishida I, Rowland O, Samuels L, Schmid KM, Wada H, Welti R, Xu C, Zallot R, Ohlrogge J (2013) Acyllipid metabolism. Arabidopsis Book. 11:e0161CrossRefGoogle Scholar
- Lin ES, Chou HJ, Kuo PL, Huang YC (2010) Antioxidant and antiproliferative activities of methanolic extracts of Perilla frutescens. Journal of Medicinal Plants Research 46:477–483Google Scholar
- Makino T (1961) Makino’s new illustrated flora of Japan. Hokuryukan, Tokyo (in Japanese)Google Scholar
- Nitta M (2001) Origin of Perilla crops and their weedy type. Ph.D. Thesis, Kyoto University, KyotoGoogle Scholar
- Nitta M, Ohnishi O (1999) Genetic relationships among two Perilla crops, shiso and egoma, and the weedy type revealed by RAPD markers. Jpn J Genet 74:43–48Google Scholar
- Yonekura-Sakakibara K, Tanaka Y, Fukuchi-Mizutani M, Fujiwara H, Fukui Y, Ashikari T, Murakami Y, Yamaguchi M, Kusumi T (2000) Molecular and biochemical characterization of a novel hydroxycinnamoyl-CoA: anthocyanin 3-O-glucoside-6′-Oacyltransferase from Perilla frutescens. Plant Cell Physiol 41:495–502PubMedCrossRefGoogle Scholar
- Yu HC, Kosuna K, Haga M (2004) Perilla: The Genus Perilla. Taylor & Francis 11 New Fetter Lane, LondonGoogle Scholar
- Wang L, Yu S, Tong C, Zhao Y, Liu Y, Song C, Zhang Y, Zhang X, Wang Y, Hua W, Li D, Li D, Li F, Yu J, Xu C, Han X, Huang S, Tai S, Wang J, Xu X, Li Y, Liu S, Varshney RK, Wang J, Zhang X (2014) Genome sequencing of the high oil crop sesame provides insight into oil biosynthesis. Genome Biol 15:R39CrossRefGoogle Scholar