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Journal of Plant Biology

, Volume 56, Issue 4, pp 232–242 | Cite as

Molecular genetic characterization of rice seed lipoxygenase 3 and assessment of its effects on seed longevity

  • Qizhang Long
  • Wenwei Zhang
  • Peng Wang
  • Wenbiao Shen
  • Tong Zhou
  • Nannan Liu
  • Ren Wang
  • Ling Jiang
  • Jiexue Huang
  • Yihua Wang
  • Yuqiang Liu
  • Jianmin WanEmail author
Original Article

Abstract

Lipoxygenases (LOXs) are enzymes involved in lipid peroxidation. Here we reported the identification, molecular and functional characterization of the gene encoding rice (Oryza sativa L.) seed LOX3 (sLOX3). Via a map-based cloning strategy we identified Os03g0700400 as the candidate gene encoding sLOX3. Further functional complementary test and biochemical characterization of the recombinant Os03g0700400 protein verified the identification. The sLOX3 gene was highly expressed in roots, moderately in embryos and very weakly in leaves, leaf sheaths and stems. Transient expression experiment (in rice protoplasts) and subsequent laser confocal microscopic analysis demonstrated that the sLOX3 protein was localized into the cytosol. We next showed that overexpression of sLOX3 in a japonica sLOX3-normal rice cultivar, Wuyunjing 7 accelerated the decrease of seed germination ability when the seeds were routinely stored, which demonstrated that sLOX3 had a negative effect on seed longevity (storability). Meanwhile, an increased occurrence of embryo decay was observed in the same transgenic seeds, suggesting that sLOX3 might negatively affect seed longevity by facilitating colonization of particular seed pathogens. Our result forwarded the understanding of the effects of 9-LOX on rice seed longevity.

Key words

Cloning Lipoxygenase Prokaryotic expression Seed longevity Subcellular localization 

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Copyright information

© Korean Society of Plant Biologists and Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Qizhang Long
    • 1
  • Wenwei Zhang
    • 1
  • Peng Wang
    • 1
  • Wenbiao Shen
    • 1
  • Tong Zhou
    • 1
  • Nannan Liu
    • 1
  • Ren Wang
    • 1
  • Ling Jiang
    • 1
  • Jiexue Huang
    • 1
  • Yihua Wang
    • 1
  • Yuqiang Liu
    • 1
  • Jianmin Wan
    • 1
    • 2
    Email author
  1. 1.State Key Laboratory for Crop Genetics & Germplasm Enhancement, Jiangsu Provincial Center of Plant Gene EngineeringNanjing Agricultural UniversityNanjingChina
  2. 2.Institute of Crop Science, The National Key Facility for Crop Gene Resources and Genetic ImprovementChinese Academy of Agricultural SciencesBeijingChina

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