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Journal of Plant Biology

, Volume 55, Issue 6, pp 469–480 | Cite as

Dehydroascorbate reductase and glutathione reductase play an important role in scavenging hydrogen peroxide during natural and artificial dehydration of Jatropha curcas seeds

  • Samar A. Omar
  • Nabil I. Elsheery
  • Hazem M. Kalaji
  • Zeng-Fu Xu
  • Song Song-Quan
  • Robert Carpentier
  • Choon-Hwan Lee
  • Suleyman I. Allakhverdiev
Original Article

Abstract

Changes in H2O2 and the main antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR) and glutathione reductase (GR), in endospermic and embryonic tissues were studied in developing and artificially dried Jatropha curcas seeds. Immature seeds were desiccation-tolerant at 80 days after flowering, as they were able to germinate fully after artificial drying on silica gel had reduced their water content to 10–12% of fresh weight. In both endospermic and embryonic tissues, H2O2 level and, consequently, lipid peroxide content, decreased during seed development as well as after artificial dehydration of developing seeds. All examined antioxidant enzymes except DHAR showed a decrease in total activity in mature stages as compared with early stages. Expression analysis of SOD genes revealed that the decrease in total SOD activities was related to the decrease in Cu/Zn-SOD expression, while the continuous activity of SOD during maturation was related to an increase in Mn-SOD expression. Artificial drying resulted in increased SOD and DHAR activity, irrespective of the developmental stage. Our results revealed weak participation of CAT and APX in H2O2 scavenging, as well as no significant alterations in GR activities either during maturation or after artificial drying. Changes in SOD and GR isoenzyme patterns occurred during maturation-related drying, but not after artificial drying. These results highlight the role of ascorbate-glutathione cycle enzymes (DHAR and GR) in H2O2 scavenging during maturation or after artificial drying of developing J. curcas seeds.

Key words

dehydroascorbate reductase glutathione reductase hydrogen peroxide Jatropha curcas scavenging 

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Copyright information

© Korean Society of Plant Biologists and Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Samar A. Omar
    • 1
    • 2
  • Nabil I. Elsheery
    • 3
  • Hazem M. Kalaji
    • 4
  • Zeng-Fu Xu
    • 1
    • 2
  • Song Song-Quan
    • 5
  • Robert Carpentier
    • 6
  • Choon-Hwan Lee
    • 7
  • Suleyman I. Allakhverdiev
    • 8
    • 9
  1. 1.Xishuangbanna Tropical Botanical GardenChinese Academy of SciencesKunmingChina
  2. 2.Graduate School of the Chinese Academy of SciencesBeijingChina
  3. 3.Agricultural Botany Department, Faculty of AgricultureTanta UniversityTantaEgypt
  4. 4.Department of Plant PhysiologyWarsaw University of Life Sciences SGGWWarsawPoland
  5. 5.Institute of BotanyChinese Academy of SciencesBeijingChina
  6. 6.Groupe de Recherche en Biologie Végétale (GRBV)Université du Québec à Trois-RivièresTrois-RivièresCanada
  7. 7.Department of Molecular BiologyPusan National UniversityBusanKorea
  8. 8.Institute of Plant PhysiologyRussian Academy of SciencesMoscowRussia
  9. 9.Institute of Basic Biological ProblemsRussian Academy of SciencesPushchino, Moscow RegionRussia

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