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Journal of Plant Biology

, 54:409 | Cite as

Taxonomic Study of Korean Scirpus L. s.l. (Cyperaceae) II: Pattern of Phenotypic Evolution Inferred from Molecular Phylogeny

  • Jongduk Jung
  • Hong-Keun ChoiEmail author
Original Research

Abstract

Scirpus L. s.l. is well known as one of the polyphyletic groups in Cyperaceae. Recent molecular phylogenetic studies clearly suggested that Scirpus s.l. should be separated into several independent genera. In this study, we intend to present the morphological variations and patterns of phenotypic evolution based on molecular phylogeny of Korean Scirpus s.l. Five genera, including 21 taxa from Korean Scirpus s.l., were examined: three species of Bolboschoenus, three species of Schoenoplectus, eight species of Schoenoplectiella including one hybrid, five species of Scirpus, and two species of Trichophorum. For morphological analyses, 23 and 48 characters were selected from vegetative and reproductive organs, respectively. Molecular phylogeny was inferred from a nuclear ribosomal internal transcribed spacer, the chloroplast rbcL gene, and the chloroplast trnL-F region. Distinct characteristics and quantitative variation was presented for identification of the five genera and their species. A pronounced pattern of morphological character change was reduction, although many other character states seem to be homoplastic. We suggest that the reduction of phenotypic characteristics has been expressed in terms of condensation of internodes, reduction of leaf blade, and simplification of inflorescence among five genera of Korean Scirpus s.l.

Keywords

Bolboschoenus Schoenoplectus Schoenoplectiella Scirpus Trichophorum Cyperaceae Molecular phylogeny Morphology Character change 

Notes

Acknowledgments

We thank the curator at the Sungshin Women’s University herbarium for permitting the examination of specimens. This work was supported by National Research Foundation of Korea grant funded by the Korean Government (MEST; NRF-2009-0088457) and is also a part of a Ph.D. dissertation for the first author in the Graduate School of Ajou University.

Supplementary material

12374_2011_9181_MOESM1_ESM.doc (166 kb)
ESM 1 (DOC 165 kb)

References

  1. Beetle AA (1944) Studies in the genus Scirpus VII. Conspectus of sections represented in the Americas. Am J Bot 31:261–265CrossRefGoogle Scholar
  2. Beetle AA (1949) Annotated list of original descriptions in Scirpus. Am Midl Nat 41:453–493CrossRefGoogle Scholar
  3. Browning J, Gordon-Gray KD, Smith SG (1995) Achene structure and taxonomy of North American Bolboschoenus (Cyperaceae). Brittonia 47:433–445CrossRefGoogle Scholar
  4. Bruhl JJ (1995) Sedge genera of the world: relationships and a new classification of the Cyperaceae. Aust Syst Bot 8:125–305CrossRefGoogle Scholar
  5. Chen DH, Ronald PC (1999) A rapid DNA minipreparation method suitable for AFLP and other PCR applications. Plant Mol Biol Report 17:53–57CrossRefGoogle Scholar
  6. Farris JS, Källersjö M, Kluge AG, Bult C (1994) Testing significance of incongruence. Cladistics 10:315–319CrossRefGoogle Scholar
  7. Goetghebeur P (1986) Genera Cyperacearum, Een bijdrage tot de kennis van de morfologie, systematiek en fylogenese van de Cyperaceae genera. University of Ghent, Belgium, Ph. DGoogle Scholar
  8. Goetghebeur P (1998) Cyperaceae. In: Kubitzki K (ed) The Families and Genera of Vascular Plants, vol 4. Springer Verlag, Berlin, pp 141–190Google Scholar
  9. Goetghebeur P, Simpson DA (1991) Cirtical notes on Actinoscirpus, Bolboschoenus, Isolepis, Phylloscirpus and Amphiscirpus (Cyperaceae). Kew Bull 46:169–178CrossRefGoogle Scholar
  10. Guibert C, Civeyrel L, Linder P (2009) Male and female separation event trapped in a species tree. Taxon 58:172–180Google Scholar
  11. Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98Google Scholar
  12. Hipp AL, Reznicek AA, Rothrock PE, Weber JA (2006) Phylogeny and classification of Carex section Ovales (Cyperaceae). Int J Plant Sci 167:1029–1048CrossRefGoogle Scholar
  13. Hitchcock CL, Cronquist A (1973) Flora of the Pacific Northwest. University of Washington Press, Seattle and LondonGoogle Scholar
  14. Hroudová Z, Zákravský P, Wójcicki JJ, Marhold K, Jarolímová V (2005) The genus Bolboschoenus (Cyperaceae) in Poland. Pol Bot J 50:117–137Google Scholar
  15. Jung J, Choi H-K (2010) Systematic rearrangement of Korean Scirpus L. s.l. (Cyperaceae) as inferred from nuclear ITS and chloroplast rbcL sequences. J Plant Biol 53:222–232CrossRefGoogle Scholar
  16. Jung J, Choi H-K (2011) Taxonomic study of Korean Scirpus L. s.l. (Cyperaceae) I. Morphology of Bolboschoenus (Asch.) Palla, Schoenoplectus (Rchb.) Palla, Schoenoplectiella Lye, Scirpus L., and Trichophorum Pers. Korean J Plant Taxon 41:16–34Google Scholar
  17. Koyama T (1958) Taxonomic study of the genus Scirpus Linne. Journal of the Faculty of Science, University of Tokyo: Section III: Botany 7:271–366Google Scholar
  18. Koyama T (1961) Classification of the family Cyperaceae (1). Journal of the Faculty of Science, University of Tokyo: Section III: botany 8:37–148Google Scholar
  19. Linnaeus C (1754) Genera Plantarum, 5th edn. Ray Society, StockholmGoogle Scholar
  20. Lye KA (2003) Schoenoplectiella Lye, gen. nov. (Cyperaceae). Lidia 6:20–29Google Scholar
  21. Maddison WP, Maddison DR (2009) Mesquite: a modular system for evolutionary analysis. Version 2.6. Available at: http://mesquiteproject.org
  22. Muasya AM, Simpson DA, Chase MW, Culham A (1998) An assessment of suprageneric phylogeny in Cyperaceae using rbcL DNA sequences. Plant Syst Evol 211:257–271CrossRefGoogle Scholar
  23. Muasya AM, Simpson DA, Chase MW (2001) Generic relationships and character evolution in Cyperus s.l. (Cyperaceae). Syst Geogr Plants 71:539–544CrossRefGoogle Scholar
  24. Muasya AM, Simpson DA, Verboom GA, Goetghebeur P, Naczi RFC, Chase MW, Smets E (2009) Phylogeny of Cyperaceae based on DNA sequence data: current progress and future prospects. Bot Rev 75:2–21CrossRefGoogle Scholar
  25. Naczi R (2009) Insights on using morphologic data for phylogenetic analysis in sedges (Cyperaceae). Bot Rev 75:67–95CrossRefGoogle Scholar
  26. Nylander JAA (2004) MrModeltest 2.3. (program distributed by the author) Evolutionary Biology Centre, Uppsala UniversityGoogle Scholar
  27. Ohwi J (1944) Cyperaceae Japonicae, II. A synopsis of the Rhynchosporoideae and Scirpoideae of Japan, including the Kuriles, Saghalin, Korea, and Formosa. Memoirs of the College of Science; Kyoto Imperial University. Series B, (botany, zoology, geology) biology 18:1–182Google Scholar
  28. Palla E (1888) Im botanischen Discussionsabende am 16 Marz sprach Herr Dr. Ed. Palla uber die Gattung Scirpus. Verhandlungen der Kaiserlich-Königlichen Zoologisch-Botanischen Gesellschaft in Wien 38:49Google Scholar
  29. Palla E (1907) Cyperaceen, In Koch WDJ (ed) Synopsis der Deutschen und Schweizer Flora, 3 edn, vol 3. Leipzig, pp 2515–2680Google Scholar
  30. Persoon CH (1805) Synopsis Plantarum, vol 1. apud C. F. Cramerum, Parisiis LutetiorumGoogle Scholar
  31. Posada D, Buckley TR (2004) Model selection and model averaging in phylogenetics: advantages of akaike information criterion and bayesian approaches over likelihood ratio tests. Syst Biol 53:793–808PubMedCrossRefGoogle Scholar
  32. Ronquist F, Huelsenbeck JP (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574PubMedCrossRefGoogle Scholar
  33. Shobe WR, Lersten NR (1967) A technique for clearing and staining Gymnosperm leaves. Botanical Gazette 128:150–152CrossRefGoogle Scholar
  34. Simmons MP, Ochoterena H (2000) Gaps as characters in sequence-based phylogenetic analyses. Syst Biol 49:369–381PubMedCrossRefGoogle Scholar
  35. Simpson DA, Muasya AM, Alves MV, Bruhl JJ, Dhooge S, Chase MW, Furness CA, Ghamkhar K, Goetghebeur P, Hodkinson TR, Marchant AD, Reznicek AA, Nieuwborg R, Roalson EH, Smets E, Starr JR, Thomas WW, Wilson KL, Zhang X (2007) Phylogeny of Cyperaceae based on DNA sequence data—a new rbcL analysis. Aliso 23:72–83Google Scholar
  36. Smith SG (1995) New combinations in North American Schoenoplectus, Bolboschoenus, Isolepis, and Trichophorum (Cyperaceae). Novon 5:97–102CrossRefGoogle Scholar
  37. Swofford DL (2002) PAUP*: Phylogenetic analysis using parsimony (*and other methods). Ver. 4.0b10. Sinauer Associates, SunderlandGoogle Scholar
  38. Taberlet P, Gielly L, Pautou G, Bouvet J (1991) Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Mol Biol 17:1105–1109PubMedCrossRefGoogle Scholar
  39. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882PubMedCrossRefGoogle Scholar
  40. University of Texas Health Science Center at San Antonio (2002) UTHSCSA ImageTool program. (Texas and available from the Internet by anonymous FTP from http://maxrad6.uthscsa.edu)
  41. White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA GD, Sninsky JJ, White TJ (eds) PCR Protocols: a guide to methods and applications. Academic, San Diego, pp 315–322Google Scholar

Copyright information

© The Botanical Society of Korea 2011

Authors and Affiliations

  1. 1.Department of Biological ScienceAjou UniversitySuwonSouth Korea

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