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Journal of Plant Biology

, Volume 54, Issue 3, pp 180–189 | Cite as

Rice Gene OsDSR-1 Promotes Lateral Root Development in Arabidopsis Under High-Potassium Conditions

  • Xu Ming Yin
  • Pedro S. C. F. Rocha
  • Man Ling Wang
  • Yu Xin Zhu
  • Luo Ye Li
  • Shu Feng Song
  • Xinjie Xia
Original Research

Abstract

Rice gene Oryza sativa Drought Stress Response-1 (OsDSR-1) was one of the genes identified to be responsive to drought stress in the panicle of rice at booting and heading stages by both microarray and quantitative real-time PCR analyses. OsDSR-1 encodes a putative calcium-binding protein, and its overexpression in Arabidopsis rendered transgenic plants to produce much shorter lateral roots (LRs) than wild-type (WT) plants in the medium supplemented with abscisic acid (ABA), suggesting that OsDSR-1 may act as a positive regulator during the process of ABA inhibition of LR development. No significant difference was observed in the total LR length between WT and transgenic plants in the media with the increase of only osmotic stress caused by NaCl, LiCl, and mannitol, while transgenic Arabidopsis seedlings appeared to produce larger root systems with longer total LR lengths under high-potassium conditions than WT seedlings. Further analysis showed that external Ca2+ was required for the production of larger root systems, indicating that the promotion by OsDSR-1 of the LR development of transgenic Arabidopsis seemed to occur in a Ca2+-dependent manner under high-potassium conditions. We propose that OsDSR-1 may function as a calcium sensor of the signal transduction pathway controlling the LR development under high-potassium conditions.

Keywords

Arabidopsis Lateral root (LR) Potassium Stress 

Notes

Acknowledgments

This research was supported by One-Hundred Person Project of the Chinese Academy of Sciences (02200420062903) and the project of Nitrogen and Phosphorus Cycling and Manipulation for Agro-Ecosystems, the Knowledge Innovation Program of the Chinese Academy of Sciences (KZCX2-YW-T07)

Supplementary material

12374_2011_9154_MOESM1_ESM.doc (187 kb)
Fig. 1 Screening of transgenic Arabidopsis plants by PCR using OsDSR-1-specific primers. M, DNA marker; line 1, wild type; lines 2–11, positive transgenic Arabidopsis (DOC 187 kb)
12374_2011_9154_MOESM2_ESM.doc (42 kb)
Fig. 2 Analysis of the expression level of OsDSR-1 in transgenic Arabidopsis by using reverse-transcription PCR with OsDSR-1 specific primers. M, DNA marker; line 1, wild type; lines 2–4, positive transgenic Arabidopsis (DOC 42 kb)
12374_2011_9154_MOESM3_ESM.doc (31 kb)
Fig. 3 Inhibition effect of LiCl on LR development. The total LR lengths of seedlings of Arabidopsis treated with different concentrations of LiCl (0, 5, and 10 mM) are shown. Data with different letters are significantly different at P = 0.05 (DOC 31 kb)
12374_2011_9154_MOESM4_ESM.doc (42 kb)
Fig. 4 Effects of NaCl and mannitol on LR development. The total LR lengths of Arabidopsis seedlings treated with different concentrations (0, 50, 75, and 100 mM) of NaCl (a) and mannitol (b) are shown. Data with different letters are significantly different at P = 0.05. The left panel represents data obtained from WT and transgenic Arabidopsis in the treatment media with CaCl2 (1.5 mM), and data obtained in the treatment media without CaCl2 are shown in the panel on the right (DOC 41 kb)

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Copyright information

© The Botanical Society of Korea 2011

Authors and Affiliations

  • Xu Ming Yin
    • 2
    • 3
  • Pedro S. C. F. Rocha
    • 2
  • Man Ling Wang
    • 2
  • Yu Xin Zhu
    • 2
  • Luo Ye Li
    • 2
  • Shu Feng Song
    • 2
    • 3
  • Xinjie Xia
    • 1
    • 2
  1. 1.Institute of Subtropical AgricultureChinese Academy of SciencesChangshaPeople’s Republic of China
  2. 2.Laboratory for Agro-ecological Process in Subtropical Region, Institute of Subtropical AgricultureCASChangshaPeople’s Republic of China
  3. 3.Graduate School of the Chinese Academy of SciencesBeijingPeople’s Republic of China

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