The role of the OsCam1-1 salt stress sensor in ABA accumulation and salt tolerance in rice
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Involvement of the salt-inducible calmodulin gene, OsCam1-1, in abscisic acid (ABA) biosynthesis during salt stress was studied in the ‘Khoa Dawk Mali 105’ (KDML105) rice cultivar (Oryza sativa L.). FL530-IL, an isogenic salt-resistant line derived from the KDML105 cultivar, accumulated a 2.9-fold higher concentration of ABA in the leaves after salt stress treatment than that for KDML105. A twenty-four and a seven- fold higher level of OsCam1-1 transcripts were detected in the leaves of the FL530-IL and KDML105 rice cultivars, respectively, after 30 min of salt stress compared to non-salt-stressed plants. Transgenic rice lines that constitutively over-express the OsCam1-1 gene were found to up-regulate ABA aldehyde oxidase and 9-cis-epoxycarotenoid dioxygenase 3, two genes involved in ABA biosynthesis, and to have a higher ABA content, when compared to the wild type and the control transgenic lines without OsCam1-1 over-expression. In addition, transgenic plants over-expressing OsCam1-1 were more tolerant to salt stress, with, for example, a better ability to maintain their shoot and root mass (as dry weight) during salt stress, than the control plants. These data indicate that OsCam1-1 signaling is likely to play an important role in ABA biosynthesis, and the level of OsCam1-1 gene expression and ABA accumulation probably contribute to salt resistance in rice.
KeywordsAbscisic acid Calmodulin Oryza sativa Rice Salt stress
calcineurin B-like protein
calcium-dependent protein kinase
Khoa Dawk Mali 105
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- Chen K, Li J, Tang J, Zhao FG, Liu X (2006) Involvement of nitric oxide in regulation of salt stress-induced ABA accumulation in maize seedling. J Plant Physiol Mol Biol 32:577–582Google Scholar
- Guo Xl, Ma YY, Liu ZH, Liu BH (2008) Effects of exterior abscisic acid on calcium distribution of mesophyll cells and calcium concentration of guard cells in maize seedlings. ASC 7:438–446Google Scholar
- Hodgson CP, Fisk RZ (1987) Hybridization probe size control: optimized “oligolabelling”. Nucleic Acids Res 1515:629Google Scholar
- Jae HY, Chan YP, Jong CK, Won DH, Mi SC, Hyeong CP, Min CK, Byeong CM, Man SC, Yun HK, Ju HL, Ho SK, Sang ML, Hae WY, Chae OL, Dae-Jin Y, Sang YL, Woo SC, Moo JC (2005) Direct interaction of a divergent CaM isoform and the transcription factor, MYB2, enhances salt tolerance in Arabidopsis. J Biol Chem 280:3697–3706Google Scholar
- Narusaka Y, Nakashima K, Shinwari ZK, Sakuma Y, Furihata T, Abe H, Narusaka M, Shinozaki K, Yamaguchi-Shinozaki K (2003) Interaction between two cis-acting elements, ABRE and DRE, in ABA-dependent expression of Arabidopsis rd29A gene in response to dehydration and high-salinity stresses. Plant J 34:137–148PubMedCrossRefGoogle Scholar
- Szepesi A, Csiszár J, Gémes K, Horváth E, Horváth F, Simon ML, Tari I (2009) Salicylic acid improves acclimation to salt stress by stimulating abscisic aldehyde oxidase activity and abscisic acid accumulation, and increases Na+ content in leaves without toxicity symptoms in Solanum lycopersicum L. J Plant Physiol 166:914–925PubMedCrossRefGoogle Scholar
- Thikart P, Kowanij D, Selanan T, Vajrabhaya M, Bangyeekhun T, Chadchawan S (2005) Genetic variation and stress tolerance of somaclonal variegated rice and its original cultivar. J Sci Res Chula Univ 30:63–75Google Scholar
- Vajrabhaya M, Vajrabhaya T (1991) Somaclonal variation of salt tolerance in rice, In Y.P.S. Bajaj, eds. Biotechnology in Agriculture and Forestry. Spring-Valege, Berlin, pp 368–382Google Scholar