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Journal of Plant Biology

, Volume 53, Issue 6, pp 433–443 | Cite as

Physical Mapping of rRNA Gene Loci and Inter-specific Relationships in Wild Lilium Distributed in Korea

  • Sayeda Sultana
  • Sang-Hoon Lee
  • Jae-Wook Bang
  • Hae-Woon Choi
Original Research

Abstract

Molecular cytogenetic analyses using fluorescence in situ hybridization (FISH) and genomic in situ hybridization (GISH) were carried out to elucidate inter-specific relationships among wild Lilium species distributed in Korea. FISH revealed four to eight 45S rRNA gene loci, which are located on chromosomes 1–7, 10, and 11 among the different species. In contrast, the 5S rRNA gene locus was conserved on the long arm of chromosome 3, occasionally with two adjacent sites on the same chromosome arm in a few species. The 5S rDNA site was located adjacent to the 45S rDNA site in only three species, Lilium distichum, Lilium hansonii, and Lilium tsingtauense. GISH analysis using genomic DNA probes detected strong hybridization of genomes between diploid and triploid Lilium lancifolium species, demonstrating that triploid plants were derived from diploid L. lancifolium and not from Lilium maximowiczii. Phylogenetic analysis of the ITS and NTS sequences supported the cytogenetic data as well as Comber’s classification of the genus Lilium.

Keywords

FISH/GISH ITS/NTS Lilium species rRNA gene 

Notes

Acknowledgements

The authors thank Dr. CS Moon for his support during the collection of plant materials. This work was supported by Basic Science Research Program through the National Research Foundation (NRF) funded by the Ministry of Education, Science and Technology of Korea (KRF-2008-313-C00717).

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Copyright information

© The Botanical Society of Korea 2010

Authors and Affiliations

  1. 1.Department of Biology, College of Biological Science and BiotechnologyChungnam National UniversityDaejeonSouth Korea

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