Transcriptomics Analysis Identified Candidate Genes Colocalized with Seed Dormancy QTLs in Rice (Oryza sativa L.)
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Rice seed dormancy is an important trait related to the preharvest sprouting resistance of rice and is controlled by a polygene network. To identify the genes involved in this process, transcriptome analysis was applied to strong seed dormancy indica cultivar N22 and its weak dormancy mutant Q4646. The results showed that 280 genes were significantly upregulated and 244 genes significantly downregulated in the seed of Q4646 as compared to N22 during 25 to 28 days after heading. These genes were mainly involved in stress response, C-compound metabolism, plant development, DNA processing, and lipid metabolism. Some of these genes were colocalized with several reported dormancy QTLs, suggesting that they are possibly candidate genes underlying rice seed dormancy. Our work provides important clues for future effort to clone seed dormant genes in rice.
KeywordsRice Seed dormancy Mutant Microarray Quantitative trait loci (QTL)
This research was supported by grants from the National Natural Science Foundation of China (No. 30471120, 30671246), 863 Program (projects 2009AA101101) of China, the Jiangsu Cultivar Development Program (projects BE2009301-3 and BE2008352), and the 111 Project (B08025).
- Ikehashi H (1972) Induction and test of dormancy of rice seeds by temperature condition during maturation. Jpn J Breed 22:209–216Google Scholar
- Jiang L, Cao YJ, Wang CM, Zhai HQ, Wan JM, Yoshimura A (2003) Detection and analysis of QTL for seed dormancy in rice (Oryza sativa L.) using RIL and CSSL population. Acta Genetica Sin 30(5):453–458 (in Chinese)Google Scholar
- Jiang L, Zhang WW, Zhai HQ, Wan JM (2005) Mapping and analysis of quantitative trait loci controlling seed dormancy in rice. Scientia Agricultura Sinica 38(4):650–656 (in Chinese)Google Scholar
- Miura K, Lin SY, Yano M, Nagamine T (2002) Mapping quantitative trait loci controlling seed longevity in rice (Oryza sativa L.). Theor Appl Genet 104:981–986Google Scholar
- Ringlund K (1993) The importance of pre-harvest sprouting research. In: Walker-Simons MK, Ried JL (eds) Pre-harvest sprouting in cereals 1992. American Association of Cereal Chemists, St. Paul, pp 3–7Google Scholar
- Tang JY, Jiang L, Wang CM, Liu SJ, Chen LM, Zhai HQ, Yoshimura A, Wan JM (2004) Analysis of QTL for seed dormancy and their response to dry heat treatment in rice (Oryza sativa L.). Scientia Agricultura Sinica 37(12):1791–1796 (in Chinese)Google Scholar