Journal of Plant Biology

, Volume 53, Issue 5, pp 330–337 | Cite as

Transcriptomics Analysis Identified Candidate Genes Colocalized with Seed Dormancy QTLs in Rice (Oryza sativa L.)

  • Huaide Qin
  • Fuqing Wu
  • Kun Xie
  • Zhijun Cheng
  • Xiuping Guo
  • Xin Zhang
  • Jie Wang
  • Cailin Lei
  • Jiulin Wang
  • Long Mao
  • Ling Jiang
  • Jianmin Wan
ORIGINAL RESEARCH

Abstract

Rice seed dormancy is an important trait related to the preharvest sprouting resistance of rice and is controlled by a polygene network. To identify the genes involved in this process, transcriptome analysis was applied to strong seed dormancy indica cultivar N22 and its weak dormancy mutant Q4646. The results showed that 280 genes were significantly upregulated and 244 genes significantly downregulated in the seed of Q4646 as compared to N22 during 25 to 28 days after heading. These genes were mainly involved in stress response, C-compound metabolism, plant development, DNA processing, and lipid metabolism. Some of these genes were colocalized with several reported dormancy QTLs, suggesting that they are possibly candidate genes underlying rice seed dormancy. Our work provides important clues for future effort to clone seed dormant genes in rice.

Keywords

Rice Seed dormancy Mutant Microarray Quantitative trait loci (QTL) 

Notes

Acknowledgments

This research was supported by grants from the National Natural Science Foundation of China (No. 30471120, 30671246), 863 Program (projects 2009AA101101) of China, the Jiangsu Cultivar Development Program (projects BE2009301-3 and BE2008352), and the 111 Project (B08025).

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Copyright information

© The Botanical Society of Korea 2010

Authors and Affiliations

  • Huaide Qin
    • 1
  • Fuqing Wu
    • 2
  • Kun Xie
    • 1
  • Zhijun Cheng
    • 2
  • Xiuping Guo
    • 2
  • Xin Zhang
    • 2
  • Jie Wang
    • 2
  • Cailin Lei
    • 2
  • Jiulin Wang
    • 2
  • Long Mao
    • 2
  • Ling Jiang
    • 1
  • Jianmin Wan
    • 1
    • 2
  1. 1.National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research CenterNanjing Agricultural UniversityNanjingChina
  2. 2.National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop ScienceChinese Academy of Agricultural SciencesBeijingChina

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