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Journal of Plant Biology

, Volume 52, Issue 5, pp 475–481 | Cite as

Arabidopsis MiR396 Mediates the Development of Leaves and Flowers in Transgenic Tobacco

  • Fengxi Yang
  • Gang Liang
  • Dongmei Liu
  • Diqiu YuEmail author
Original Research

Abstract

MicroRNAs (miRNAs) are single-stranded, noncoding small RNAs that usually function as posttranscriptional negative regulators by base pairing to target genes. They are pivotal to plant development. MiR396 is conserved among plant species and is predicted to target GRF (growth-regulating factor) genes in Arabidopsis. Here, overexpression of ath-miR396 in tobacco reduced the levels of three NtGRF-like genes containing an miR396 match site. Furthermore, its elevated expression resulted in a small, narrow leaf phenotype similar to that found with the Arabidopsis grf1grf2grf3 triple mutant. We also demonstrated that 35S:MIR396a transgenic plants were defective in the four whorls of floral organs. These results provide a link between the miR396-mediated regulatory pathway of NtGRF-like gene expression and the developmental processes for leaves and flowers in tobacco.

Keywords

GRF miR396 Narrow leaf Tobacco 

Notes

Acknowledgments

This research was supported by the National High Technology Research and Development Program of China (863 Program; 2006AA02Z129), the National Natural Science Foundation of China (90408022), the Science Foundation of Yunnan Province (2004C0051M), and the “Hundred Talents” Program of the Chinese Academy of Sciences.

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Copyright information

© The Botanical Society of Korea 2009

Authors and Affiliations

  • Fengxi Yang
    • 1
    • 2
  • Gang Liang
    • 1
    • 2
  • Dongmei Liu
    • 1
  • Diqiu Yu
    • 1
    Email author
  1. 1.Xishuangbanna Tropical Botanical GardenChinese Academy of SciencesKunmingPeople’s Republic of China
  2. 2.The Graduate School of the Chinese Academy of SciencesBeijingPeople’s Republic of China

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