Journal of Plant Biology

, Volume 52, Issue 5, pp 453–461 | Cite as

Overexpression of AtNHX1, a Vacuolar Na+/H+ Antiporter from Arabidopsis thalina, in Petunia hybrida Enhances Salt and Drought Tolerance

Original Research

Abstract

Plant Na+/H+ antiporter plays a critical role in plant salt tolerance. In this study, AtNHX1, a vacuolar Na+/H+ antiporter from Arabidopsis thalina, was introduced into Petunia hybrida using Agrobacterium-mediated methods. Polymerase chain reaction (PCR) and DNA gel blot analysis confirmed that AtNHX1 was successfully integrated into the P. hybrida genomes. Reverse transcription (RT)-PCR analysis indicated that AtNHX1 expressed highly in transgenic plants. It was found that the 35S::AtNHX1 transgenic P. hybrida plants showed much more resistant to high concentrations of NaCl and to water deprivation than the isogenic wild-type (WT) Petunia plants. The transgenic plants accumulated more Na+, K+, and proline in their leaf tissue than that of the WT Petunia plants and maintained high water contents and high ratio of K+/Na+. These results demonstrated that overexpression of the vacuolar Na+/H+ antiporter conferred both salt tolerance and drought tolerance to the transgenic Petunia plants.

Keywords

Drought tolerance Petunia hybrida Salt tolerance Transgenic plants Vacuolar Na+/H+ antiporter 

Abbreviations

AtNHX1

A vacuolar Na+/H+ antiporter of Arabidopsis

CaMV

Cauliflower mosaicvirus

HYG

Hygrimycin phosphotransferase

MDA

Malonyldialdehyde

MS

Murashige and Skoog

RT

Reverse transcription

RWC

Relative water content

WT

Wild type

Notes

Acknowledgments

We thank Prof. Blumwald E (Department of Plant Sciences, UC Davis) for revising the manuscript. This work was financially supported by Basic Research Project of Shanghai and National Natural Science Foundation of China.

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Copyright information

© The Botanical Society of Korea 2009

Authors and Affiliations

  1. 1.School of Life ScienceEast China Normal UniversityShanghaiPeople’s Republic of China
  2. 2.Shanghai Agrobiological Gene CenterShanghaiPeople’s Republic of China

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