Journal of Plant Biology

, Volume 52, Issue 1, pp 41–48 | Cite as

Flavonoid O-Diglucosyltransferase from Rice: Molecular Cloning and Characterization

  • Bong-Gyu Kim
  • Na Yeon Kim
  • Jeong Ho Kim
  • Kazuya Akimitsu
  • Youhoon Chong
  • Joong-Hoon Ahn
Original Research
First Online:
Received:
Revised:
Accepted:

Abstract

Among more than 100 rice uridine diphosphate glycosyltransferases (UGTs), OsUGT-3 was selected as a candidate for producing flavonoid O-diglycosyltransferases based on phylogenetic analysis and molecular docking. This gene was functionally expressed in Escherichia coli. Analysis of kaempferol, luteolin, quercetin, and tricin reaction products using liquid chromatography-mass spectrometry revealed that these were diglucosylated. The glucosylation positions of kaempferol, which was the best substrate, were determined to be the 3- and 7-hydroxyl groups. This is the first flavonoid O-diglucosyltransferase described from rice.

Keywords

Flavonoids Flavonoid O-diglucoside Glycosyltransferase Oryza sativa UGT 
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Notes

Acknowledgments

This work was supported by a Korea Research Foundation grant funded by the Korean Government (MOEHRD) (KRF-2005-214-00029).

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Copyright information

© The Botanical Society of Korea 2009

Authors and Affiliations

  • Bong-Gyu Kim
    • 1
  • Na Yeon Kim
    • 1
  • Jeong Ho Kim
    • 1
  • Kazuya Akimitsu
    • 2
  • Youhoon Chong
    • 1
  • Joong-Hoon Ahn
    • 1
  1. 1.Department of Bioscience and Biotechnology, Bio/Molecular Informatics CenterKonkuk UniversitySeoulSouth Korea
  2. 2.Laboratory of Plant Pathology, United Graduate School and Faculty of AgricultureKagawa UniversityMikiJapan

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