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Genetic Diversity and Phylogenetic Relationship of Saccharum spontaneum L. with Different Ploidy Levels Based on SRAP Markers

  • Xing-Hua Yu
  • Xian-Hong WangEmail author
  • Qing-Hui YangEmail author
Research Article
  • 31 Downloads

Abstract

Saccharum spontaneum L. is an important sugarcane germplasm resource with excellent properties of cold, drought, barren, insect and disease resistance. To improve the utilization efficiency of S. spontaneum in sugarcane breeding and variety improvement, the genetic diversity and phylogenetic relationship of 70 S. spontaneum species with different ploidy levels (2n = 40, 48, 54, 64, 80, 88, 92 and 96) from Tibet of China, Nepal, and Myanmar were analyzed using sequence-related amplified polymorphism markers and capillary electrophoresis. The results showed that the average number of polymorphic loci and the proportion of polymorphic loci in polymerase chain reaction amplification results using 16 pairs of primers were 77.19 and 95.22%, respectively. There were significant genetic diversities among different ploidy levels of S. spontaneum. The coefficient of gene differentiation (Gst) and gene flow (Nm) of different ploidy levels of S. spontaneum were 0.31 and 1.11, respectively, suggesting that there is significant genetic differentiation among S. spontaneum from different geographical sources and that gene exchanges have occurred among S. spontaneum with different ploidy levels. Cluster analysis showed that S. spontaneum of the same ploidy level tended to cluster together, and variance analysis showed that intra-specific variation was significantly higher than inter-specific variation in S. spontaneum with different ploidy levels. The results showed that S. spontaneum from Tibet China, Nepal and Myanmar demonstrates significant genetic diversity and that gene exchanges have occurred among S. spontaneum with different ploidy levels. The ploidy evolution of germplasms might be a mesh-type development rather than a single direction.

Keywords

Sugarcane Saccharum spontaneum L. SRAP Genetic diversity Phylogenetic relationship 

Notes

Acknowledgements

The research described in here was supported by the National Science Foundation of China (31260348, 31460372 and 31760417).

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Copyright information

© Society for Sugar Research & Promotion 2019

Authors and Affiliations

  1. 1.Sugarcane Research InstituteYunnan Agricultural UniversityKunmingPeople’s Republic of China
  2. 2.Wenshan Academy of Agricultural SciencesWenshanPeople’s Republic of China

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