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Sugar Tech

, Volume 19, Issue 2, pp 136–147 | Cite as

Intercropping in Sugarcane Cultivation Influenced the Soil Properties and Enhanced the Diversity of Vital Diazotrophic Bacteria

  • Manoj Kumar Solanki
  • Zhen Wang
  • Fei-Yong Wang
  • Chang-Ning Li
  • Tao-Ju Lan
  • Rajesh Kumar Singh
  • Pratiksha Singh
  • Li-Tao Yang
  • Yang-Rui Li
Research Article

Abstract

Soil survey was conducted at four different locations of Guangxi, China to investigate the effect of peanut and soybean inter-cropping in sugarcane cultivation on the soil properties and diazotrophic bacterial diversity. Principal component analysis result shown (PCA) showed difference impact of intercropping and monoculture on the basis of biological and chemical properties of soil. Two-way analysis of variance of soil properties showed a significant interactive effect with locations and cultivation systems. Microbial enumeration results demonstrated that intercropping system enhanced the diazotrophic population. After isolation of diazotrophs, 21 selected bacteria were characterized by siderophore, phosphate, indole acetic acid, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase and nitrogenase assay. Among the identified bacteria, isolate MYSP104 showed maximum nitrogenase activity and isolate MYSS78 showed maximum ACC deaminase activity. Partial 16S rRNA gene sequencing results classified these diazotrophs in six phyla (Firmicutes, α, β, Υ-proteobacteria, actinobacteria and bacteroidetes). Diazotrophs such as Bacillus tequilensis, Variovorax paradoxus, Acidovorax facilis, Leucobacter aridicollis, Streptomyces fimicarius and Pseudomonas nitroreducens were reported for the first time from the sugarcane rhizosphere of Guangxi, China. Venn diagram explained that seven bacterial species (Brevibacterium, Burkholderia, Delftia, Leucobacter, Pseudomonas, Sinorhizobium and Variovorax) were recognized with soybean and sugarcane intercropping cultivation systems. This study concluded that intercropping system could enhance the population of N-fixers in soil. Soybean intercropping influenced the soil chemical and biological properties better than peanut. Moreover, the isolated diazotrophs need further characterization and they might be utilized as bio-inoculums for commercial sugarcane production in the future.

Keywords

Sugarcane Intercropping Diazotrophs Nitrogenase Peanut Soybean Principal component analysis 

Notes

Acknowledgments

This present study was supported by the grants from the National High Technology Research and Development Program ("863" Program) of China (2013AA102604), National Natural Science Foundation of China (31171504, 31101122, 31471449), Guangxi Special Funds for Bagui Scholars’ and Distinguished Experts (2013), Guangxi Natural Science Foundation (2011GXNSFF018002, 2012GXNSFDA053011, 2013NXNSFAA019073) and Guangxi Academy of Agriculture Sciences Fund (GNK2014YD01, GNKB2014021).

Compliance with Ethical Standards

Conflict of interest

No conflict of interest declared.

Supplementary material

12355_2016_445_MOESM1_ESM.docx (22 kb)
Supplementary material 1 (DOCX 22 kb)

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Copyright information

© Society for Sugar Research & Promotion 2016

Authors and Affiliations

  • Manoj Kumar Solanki
    • 1
  • Zhen Wang
    • 3
  • Fei-Yong Wang
    • 1
    • 2
    • 3
  • Chang-Ning Li
    • 1
    • 2
  • Tao-Ju Lan
    • 1
  • Rajesh Kumar Singh
    • 3
  • Pratiksha Singh
    • 3
  • Li-Tao Yang
    • 3
  • Yang-Rui Li
    • 1
    • 2
  1. 1.Guangxi Crop Genetic Improvement and Biotechnology LabGuangxi Academy of Agricultural SciencesNanningChina
  2. 2.Guangxi Key Laboratory of Sugarcane Genetic Improvement, Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture, Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Sugarcane Research CenterChinese Academy of Agricultural SciencesNanningChina
  3. 3.State Key Laboratory for Conservation and Utilization of Subtropical Agro-bio Resources, Agricultural CollegeGuangxi UniversityNanningChina

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