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Sugar Tech

, Volume 19, Issue 1, pp 17–25 | Cite as

A Novel Non-specific Lipid Transfer Protein Gene from Sugarcane (NsLTPs), Obviously Responded to Abiotic Stresses and Signaling Molecules of SA and MeJA

  • Yun Chen
  • Jingjing Ma
  • Xu Zhang
  • Yuting Yang
  • Dinggang Zhou
  • Qing Yu
  • Youxiong Que
  • Liping Xu
  • Jinlong Guo
Research Article

Abstract

Non-specific lipid transfer proteins (NsLTPs) are soluble, small, basic proteins in plant, which have been reported to be involved in plant physiological functions such as the catalyzing transfer of phospholipids and play an important role in plant defense and stress responses. In this study, a member of NsLTP family gene (ScNsLTP, Acc. No. KR259657) was isolated from a full-length cDNA library of sugarcane stalk. The cDNA of ScNsLTP was 671 bp long and contained a 312 bp open reading frame (ORF), which can encode a protein of 103 amino acid residues with molecular weight of 10.66 kDa. The ScNsLTP transcript levels in sugarcane seedlings decreased in response to SA, whereas it increased under MeJA treatment, suggesting an antagonistic regulatory mechanism between the signaling molecules of SA and MeJA. The transcript levels of ScNsLTP were obviously up-regulated under chilling and PEG stresses, implying that the ScNsLTP gene was involved in response to abiotic stresses and playing a positive role in adaption to low temperature and drought stresses. These results provide important information for further functional studies of plant NsLTPs gene.

Keywords

Sugarcane (Saccharum hybrid complex) Lipid transfer proteins (LTPs) Gene expression qRT-PCR 

Notes

Acknowledgments

This study was funded by the National Natural Science Foundation of China (Grant Numbers 31271782, 31340060) and the Foundation for Development of Science and Technology from Fujian Agriculture and Forestry University (2016), China.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

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Supplementary material 3 (BMP 943 kb)
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Supplementary material 4 (DOCX 13 kb)

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Copyright information

© Society for Sugar Research & Promotion 2016

Authors and Affiliations

  • Yun Chen
    • 1
  • Jingjing Ma
    • 1
  • Xu Zhang
    • 1
  • Yuting Yang
    • 1
  • Dinggang Zhou
    • 1
  • Qing Yu
    • 1
  • Youxiong Que
    • 1
  • Liping Xu
    • 1
  • Jinlong Guo
    • 1
  1. 1.Key Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of AgricultureFujian Agriculture and Forestry UniversityFuzhouPeople’s Republic of China

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