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Sugar Tech

, Volume 17, Issue 4, pp 348–355 | Cite as

Better Osmotic Adjustment Mediates Salt and PEG Stress Tolerance in Primed Plants of Contrasting Cultivars of Sugarcane

  • Vikas Yadav Patade
  • Sujata Bhargava
  • Penna SuprasannaEmail author
Research Article
  • 294 Downloads

Abstract

Priming improves germination, vigour and performance under environmental conditions in seed crops; however, priming mediated stress tolerance is not much understood in vegetatively propagated crop plants. Here we report growth and physiological characteristics in salt primed sugarcane plants upon 15 days exposure to salt (NaCl, 150 mM) or iso-osmotic (−0.7 MPa) polyethylene glycol-PEG 8000 (20 % w/v) stress in contrasting cultivars: salt sensitive CoC 671 and tolerant Co 62175. Priming led to variable growth, osmolyte accumulation and antioxidant capacity. Priming could not further improve tolerance to salt or PEG stress in salt tolerant cultivar, while the primed plants of the salt sensitive cultivar showed improved tolerance. Priming led to improved shoot growth, reduced leaf senescence and better osmotic adjustment through accumulation of glycine betaine and dissolved ionic solutes in sensitive cultivar upon exposure to salt or PEG stress. The results suggest that osmotic adjustment plays an important role in increasing the stress tolerance of primed sugarcane plants.

Keywords

Salt priming Iso-osmotic stress Osmotic adjustment Antioxidant defence 

Notes

Acknowledgments

Thanks are extended to University Grant Commission, New Delhi, India and Department of Botany, University of Pune, Pune, India for financial support in terms of Research Fellowship to the senior author.

References

  1. Afzal, I., S.M.A. Basra, A. Hameed, and M. Farooq. 2006. Physiological enhancements for alleviation of salt stress in Wheat. Pakistan Journal of Botany 385: 1649–1659.Google Scholar
  2. Bartels, D., and R. Sunkar. 2005. Drought and salt tolerance in plants. Critical Reviews in Plant Sciences 241: 23–58.CrossRefGoogle Scholar
  3. Beckers, G.J.M., and U. Conrath. 2007. Priming for stress resistance: From the lab to the field. Current Opinion in Plant Biology 10: 425–431.CrossRefPubMedGoogle Scholar
  4. Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72: 248–254.CrossRefPubMedGoogle Scholar
  5. Casenave, E.C., and M.E. Toselli. 2007. Hydropriming as a pre-treatment for cotton germination under thermal and water stress conditions. Seed Science and Technology 35: 88–98.CrossRefGoogle Scholar
  6. Conrath, U. 2011. Molecular aspects of defence priming. Trends in Plant Science 16: 524–531.CrossRefPubMedGoogle Scholar
  7. Foti, R., K. Aburenia, A. Tigerea, J. Gotosab, and J. Gerec. 2008. The efficacy of different seed priming osmotica on the establishment of maize Zea mays L. caryopses. Journal of Arid Environments 72: 1127–1130.CrossRefGoogle Scholar
  8. Ghassemi-Golezani, K., and B. Esmaeilpour. 2008. The effect of salt priming on the performance of differentially matured cucumber Cucumis sativus seeds. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 36: 67–70.Google Scholar
  9. Ghassemi-Golezani, K., A.A. Aliloo, M. Valizadeh, and M. Moghaddam. 2008. Effects of hydro and osmo-priming on seed germination and field emergence of lentil Lens culinaris Medik. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 36: 29–33.Google Scholar
  10. Ghiyasi, M., A.A. Seyahjani, M. Tajbakhsh, R. Amirnia, and H. Salehzadeh. 2008. Effect of osmopriming with polyethylene glycol 8000 on germination and seedling growth of wheat Triticum aestivum L. seeds under salt stress. Research Journal of Biological Sciences 310: 1249–1251.Google Scholar
  11. Goh, C.H., H.G. Nam, and Y.S. Park. 2003. Stress memory in plants: A negative regulation of stomatal response and transient induction of rd22 gene to light in abscisic acid entrained Arabidopsis plants. Plant Journal 36: 240–255.CrossRefPubMedGoogle Scholar
  12. Harris, D., A. Joshi, P.A. Khan, P. Gothkar, and P.S. Sodhi. 1999. On farm seed priming in semi-arid agriculture: Development and evaluation in maize, rice and chickpea in India using participatory methods. Experimental Agriculture 35: 15–29.CrossRefGoogle Scholar
  13. Iqbal, M., and M. Ashraf. 2007. Seed treatment with auxins modulates growth and ion partitioning in salt-stressed wheat plants. Journal of Integrative Plant Biology 49: 1003–1015.CrossRefGoogle Scholar
  14. Jisha, K.C., K. Vijayakumari, and J.T. Puthur. 2013. Seed priming for abiotic stress tolerance: An overview. Acta Physiologiae Plantarum 35(3): 1381–1396.CrossRefGoogle Scholar
  15. Kaur, S., A.K. Gupta, and N. Kaur. 2005. Seed Priming increases crop yield possibly by modulating enzymes of sucrose metabolism in chickpea. Journal of Agronomy and Crop Science 191: 81–87.CrossRefGoogle Scholar
  16. Musa, A.M., D. Harris, C. Johansen, and J. Kumar. 2001. Short duration chickpea to replace fallow after Aman Rice: The role of on-farm seed priming in the high Barind Tract of Bangladesh. Experimental Agriculture 37: 509–521.CrossRefGoogle Scholar
  17. Neto, A.D.A., J.T. Prisco, J. Enéas-Filho, J.V.R. Medeiros, and E. Gomes-Filho. 2005. Hydrogen peroxide pre-treatment induces salt stress acclimation in maize plants. Journal of Plant Physiology 162: 1114–1122.CrossRefGoogle Scholar
  18. Parra, M., A. Albacete, C. Martínez-Andújar, and F. Pérez-Alfocea. 2007. Increasing plant vigour and tomato fruit yield under salinity by inducing plant adaptation at the earliest seedling stage. Environmental and Experimental Botany 60: 77–85.CrossRefGoogle Scholar
  19. Patade, V.Y., S. Bhargava, and P. Suprasanna. 2009. Halopriming imparts tolerance in sensitive sugarcane cultivar to salt and PEG induced drought stress. Agriculture, Ecosystems and Environment 134: 24–28.CrossRefGoogle Scholar
  20. Patanea, C., C. Valeria, and L.C. Salvatore. 2009. Germination and radicle growth in unprimed and primed seeds of sweet sorghum as affected by reduced water potential in NaCl at different temperatures. Industrial Crops and Products 30: 1–8.CrossRefGoogle Scholar
  21. Patade, V.Y., Maya Kumari, and Zakwan Ahmed. 2011a. Chemical seed priming as a simple technique to impart cold and salt stress tolerance in Capsicum. Journal of Crop Improvement 25: 497–503.CrossRefGoogle Scholar
  22. Patade, V.Y., S. Bhargava, and P. Suprasanna. 2011b. Salt and drought tolerance of sugarcane under iso-osmotic salt and water stress: Growth, osmolytes accumulation and antioxidant defense. Journal of Plant Interactions 6(3): 275–282.CrossRefGoogle Scholar
  23. Patade, V.Y., Maya Kumari, and Zakwan Ahmed. 2012a. Chemical seed priming as an efficient approach for developing cold tolerance in Jatropha. Journal of Crop Improvement 26: 140–149.CrossRefGoogle Scholar
  24. Patade, V.Y., S. Bhargava, and P. Suprasanna. 2012b. Halopriming mediated salt and iso-osmotic PEG stress tolerance and, gene expression profiling in sugarcane (Saccharum officinarum L.). Molecular Biology Reports 39: 9563–9572.CrossRefPubMedGoogle Scholar
  25. Raven, J.A. 1985. Regulation of pH and generation of osmolarity in vascular plants: A cost benefit analysis in relation to efficiency of use of energy, nitrogen and water. New Phytologist 101: 25–77.CrossRefGoogle Scholar
  26. Rozbeh, F., and S. Farzad. 2006. The effects of NaCl priming on salt tolerance in canola Brassica napus L. seedlings grown under saline conditions. Indian Journal of Crop Science 11: 74–78.Google Scholar
  27. Saed-Moocheshi, A., A. Shekoofa, H. Sadeghi, and M. Pessarakli. 2014. Drought and salt stress mitigation by seed priming with KNO3 and urea in various maize hybrids: An experimental approach based on enhancing antioxidant responses. Journal of Plant Nutrition 37: 674–689.CrossRefGoogle Scholar
  28. Sani, E., P. Herzyk, G. Perrella, V. Colot, and A. Amtmann. 2013. Hyperosmotic priming of Arabidopsis seedlings establishes a long-term somatic memory accompanied by specific changes of the epigenome. Genome Biology 14(6): R59.Google Scholar
  29. Sivritepe, N., H.O. Sivritepe, and A. Eris. 2003. The effects of NaCl priming on salt tolerance in melon seedlings grown under saline conditions. Scientia Horticulturae 97: 229–237.CrossRefGoogle Scholar
  30. Sung, D.Y., F. Kaplan, K.J. Lee, and C.L. Guy. 2003. Acquired tolerance to temperature extremes. Trends in Plant Science 8: 179–187.CrossRefPubMedGoogle Scholar
  31. Ventura, L., M. Donà, A. Macovei, D. Carbonera, A. Buttafava, A. Mondoni, G. Rossi, and A. Balestrazzi. 2012. Understanding the molecular pathways associated with seed vigor. Plant Physiology and Biochemistry 60: 196–206.CrossRefPubMedGoogle Scholar
  32. Wahid, A., A. Noreen, S.M.A. Basra, S. Gelani, and M. Farooq. 2008. Priming-induced metabolic changes in sunflower Helianthus annuus achenes improve germination and seedling growth. Botanical Studies 49: 343–350.Google Scholar

Copyright information

© Society for Sugar Research & Promotion 2014

Authors and Affiliations

  • Vikas Yadav Patade
    • 1
  • Sujata Bhargava
    • 2
  • Penna Suprasanna
    • 3
    Email author
  1. 1.Molecular Biology and Genetic Engineering DivisionDefence Institute of Bio-Energy ResearchHaldwaniIndia
  2. 2.Botany DepartmentUniversity of PunePuneIndia
  3. 3.Nuclear Agriculture and Biotechnology DivisionBhabha Atomic Research CentreMumbaiIndia

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