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Sugar Tech

, Volume 13, Issue 2, pp 174–177 | Cite as

Callogenesis and Shoot Organogenesis from Flowers of Stevia rebaudiana (Bert.)

  • Nisar Ahmad
  • Hina Fazal
  • Roshan Zamir
  • Shahid Akbar Khalil
  • Bilal Haider Abbasi
Short Communication

Abstract

The callogenic and shoot organogenic potential of the medicinaly important plant, S. rebaudiana were investigated. Callus induction and shoot regeneration were induced from flower explants of greenhouse plants incubated on Murashige and Skoog (MS)-medium supplemented with different plant growth regulators (PGRs). The best callogenic response was observed on explants incubated on MS-medium supplemented with 2.0 mg/l 6-benzyladenine (BA) along with 2.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) after 30-days of culture. Subsequent transfer of callogenic explants onto MS-medium supplemented with 2.0 mg/l BA gave 85.2% shoot organogenesis after 30-days following culture. Maximum number (21.6) of shoots/explant was recorded for 2.0 mg/l BA. Subsequent sub culturing of shoots after 3 weeks of culture, on medium with similar compositions of PGRs gave 5.1 cm longest shoot. The palynology of S. rebaudiana has also been investigated.

Keywords

Stevia rebaudiana Callogenesis Shoot organogenesis Flowers Palynology 

Abbreviations

BA

6-Benzyladenine

GA3

Gibberellic acid

NAA

α-Naphthaleneacetic acid

2,4-D

2,4-dichlorophenoxyacetic acid

PGRs

Plant growth regulators

MS0

MS medium without plant growth regulators

SEM

Scanning Electron Microscopy

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Copyright information

© Society for Sugar Research & Promotion 2011

Authors and Affiliations

  • Nisar Ahmad
    • 1
    • 2
  • Hina Fazal
    • 3
  • Roshan Zamir
    • 2
  • Shahid Akbar Khalil
    • 2
  • Bilal Haider Abbasi
    • 1
  1. 1.Department of Biotechnology, Faculty of Biological SciencesQuaid-i-Azam UniversityIslamabadPakistan
  2. 2.Nuclear Institute for Food and AgricultureTarnab PeshawarPakistan
  3. 3.Pakistan Council of Scientific and Industrial Research (PCSIR) Laboratories ComplexPeshawarPakistan

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