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Sugar Tech

, Volume 13, Issue 1, pp 27–35 | Cite as

Use of Sodium Hypochlorite as Media Sterilant in Sugarcane Micropropagation at Commercial Scale

  • R. A. SawantEmail author
  • P. N. Tawar
Research Article

Abstract

Sodium hypochlorite has been successfully utilized as media sterilant (at total active chlorine concentration 0.002% in the medium) in production of sugarcane plantlets by applying apical meristem culture technique. The process has been scaled up to produce 2.5 million sugarcane plantlets per year. Use of sodium hypochlorite in place of autoclaving has resulted in reducing considerable amount of electricity cost and ultimately reducing the cost of production of sugarcane plantlets.

Keywords

Autoclaving Cost reduction Micropropagation Sodium hypochlorite Sugarcane Shooting medium Rooting medium 

Notes

Acknowledgements

The authors are grateful to Dr. N. M. Pant, Ex Biotechnologist, VSI, Pune for generous help and encouragement. Thanks are due to Shri V. P. Rane, Ex Director General and Shri Shivajirao Deshmukh Director General VSI, Pune, for providing necessary facilities to carry out the research work.

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Copyright information

© Society for Sugar Research & Promotion 2011

Authors and Affiliations

  1. 1.Tissue Culture SectionVasantdada Sugar Institute, ManjariPuneIndia

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