Use of Sodium Hypochlorite as Media Sterilant in Sugarcane Micropropagation at Commercial Scale
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Sodium hypochlorite has been successfully utilized as media sterilant (at total active chlorine concentration 0.002% in the medium) in production of sugarcane plantlets by applying apical meristem culture technique. The process has been scaled up to produce 2.5 million sugarcane plantlets per year. Use of sodium hypochlorite in place of autoclaving has resulted in reducing considerable amount of electricity cost and ultimately reducing the cost of production of sugarcane plantlets.
KeywordsAutoclaving Cost reduction Micropropagation Sodium hypochlorite Sugarcane Shooting medium Rooting medium
The authors are grateful to Dr. N. M. Pant, Ex Biotechnologist, VSI, Pune for generous help and encouragement. Thanks are due to Shri V. P. Rane, Ex Director General and Shri Shivajirao Deshmukh Director General VSI, Pune, for providing necessary facilities to carry out the research work.
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