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Sugar Tech

, Volume 10, Issue 2, pp 154–157 | Cite as

Differential accumulation of 3-deoxy anthocyanidin phytoalexins in sugarcane varieties varying in red rot resistance in response to Colletotrichum falcatum infection

  • P. Malathi
  • R. ViswanathanEmail author
  • P. Padmanaban
  • D. Mohanraj
  • V. Ganesh Kumar
  • K. P. Salin
Research Article

Abstract

Sugarcane synthesizes a complex mixture of phytoalexins in response to inoculation with Colletotrichum falcatum Went, causative agent of red rot. To determine induction and accumulation of these compounds, field grown sugarcane plants of two varieties viz., Co 93009 and CoC 671 which are resistant and susceptible to red rot respectively, were inoculated with C. falcatum and tissue extracts were analyzed by HPLC at different intervals after inoculation for the presence of phytoalexins. Analyses of HPLC profiles revealed the induction of five major compounds in response to pathogen inoculation/injury. Among the five detected compounds only two were found to be induced specifically due to pathogen inoculation. One of the two pathogen induced compounds was found to be accumulated only in the resistant variety but completely absent in the susceptible variety. Using standards, the two pathogen induced compounds were identified as luteolinidin and apigeninidin and the results revealed that these phytoalexins induced specifically in red rot resistant variety in response to attempted pathogen infection and susceptible variety failed to synthesize these compounds. The results suggest the possible role of these compounds in red rot resistance.

Keywords

Phytoalexins 3-deoxyanthocyanidins luteolinidin apigeninidin red rot resistance sugarcane 

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References

  1. Brinker AM, Seigler DS (1991) Isolation and identification of piceatannol as a phytoalexin from sugarcane. Phytochemistry 30: 3229–3232.CrossRefGoogle Scholar
  2. Brinker AM, Seigler DS (1993) Time course of piceatannol accumulation in resistant and susceptible sugarcane stalks after inoculation with Colletotrichum falcatum. Physiol Mol Pl Pathol 42:169–176.CrossRefGoogle Scholar
  3. Brisson LF, Tenhaken R, Lamb C (1994) Functions of oxidative cross-linking of cell wall structural protein in plant disease resistance. Plant Cell 6:1703–1712.CrossRefGoogle Scholar
  4. Bruce RJ, West CA (1988) Elicitation of lignin biosynthesis and isoperoxidase activity by pectic fragments in suspension cultures of castor bean. Pl Physiol 91: 889–897.CrossRefGoogle Scholar
  5. Clifford MN (2000) Anthocyanins — nature, occurrence and dietary burden. J Sci Food Agri 80: 1063–1072.CrossRefGoogle Scholar
  6. Degra L, Salvi G, Marrioti D, De-Lorenzo D, Cervone F (1988) A polygalacturonase-inhibiting protein in alfalfa callus cultures. J Pl Physiol 133: 364–371.CrossRefGoogle Scholar
  7. Dixon RA, Paiva NL (1995) Stress-induced phenylpropanoid metabolism. Plant Cell 7: 1085–1097.CrossRefGoogle Scholar
  8. Godshall MA, Lonergan TA (1987) The effect of sugarcane extracts on the growth of the pathogenic fungus, Colletotrichum falcatum. Physiol Mol Pl Pathol 30: 299–308.CrossRefGoogle Scholar
  9. Gous F (1989) Tannins and phenols in black sorghum. Ph.D Dissertation, Texas A&M University College Station, Texas, USA.Google Scholar
  10. Kombrink E, Hahlbrock K, Hinze K, Schroder M (1991) Molecular responses of potato to infection by Phytopthora infestans. In: Smith CJ (ed) Biochemistry and Molecular Biology of Plant Pathogen Interactions. Oxford University Press, Oxford, UK, pp237–254.Google Scholar
  11. Mehdy MC (1994) Active oxygen species in plant defense against pathogens. Pl Physiol 105: 467–472.CrossRefGoogle Scholar
  12. Nicholson RL, Kollipara SS, Vincent JR, Lyons PC, Cadena-Gomez G (1987) Phytoalexin synthesis by the sorghum mesocotyl in response to infection by pathogenic and nonpathogenic fungi. Proc Natl Acad Sci USA 84: 5520–5524.CrossRefGoogle Scholar
  13. Nicholson RL, Hammerschmidt R (1992). Phenolic compounds and their role in disease resistance. Ann Rev Phytopathol 30:369–389.CrossRefGoogle Scholar
  14. Nip WK, Burns EE (1969) Pigment characterization in grain sorghum. I. Red varieties. Cereal Chem 46: 490–495.Google Scholar
  15. Nip, W. K., Burns, E. E. (1971). Pigment characterization in grain sorghum. II. white varieties. Cereal Chem., 48: 74–80.Google Scholar
  16. Smith CJ (1996) Accumulation of phytoalexins: defense mechanism and stimulus response system. New Phytol 132: 1–45.CrossRefGoogle Scholar
  17. Sweeny JG, Iacobucci GA (1981) Synthesis of anthocyanidins-III. Tetrahedron 37: 1481–1483.CrossRefGoogle Scholar
  18. Snyder BA, Nicholson RL (1990) Synthesis of phytoalexins in sorghum as a site specific response to fungal ingress. Science 248:1637–1639.CrossRefGoogle Scholar
  19. Snyder BA, Leite B, Hipskind J, Butler LG, Nicholson RL (1991) Accumulation of sorghum phytoalexins induced by Colletotrichum graminicola at the infection site. Physiol Mol Pl Pathol 39: 463–470.CrossRefGoogle Scholar
  20. Sze-Chung CL, Nicholson RL (1998) Reduction of light-induced anthocyanin accumulation in inoculated sorghum mesocotyls. Pl Physiol 116: 979–989.CrossRefGoogle Scholar
  21. Van Loon LC, Pierpoint WS, Boller TH, Conejero V (1994). Recommendations for naming plant pathogenesis-related proteins. Pl Mol Biol Reporter 12: 254–264.Google Scholar
  22. Viswanathan R, Mohanraj D, Padmanaban P, Alexander KC (1994) Possible role of red rot pigments in host-pathogen interaction in sugarcane with red rot pathogen. Indian Phytopathol 47: 281.Google Scholar
  23. Viswanathan R, Mohanraj D, Padmanaban P (1996a). Role of red rot pigments in relation to red rot resistance in sugarcane. Indian J Sugarcane Technol 11: 151–154.Google Scholar
  24. Viswanathan R, Mohanraj D, Padmanaban P, Alexander KC (1996b). Synthesis of phytoalexins in sugarcane in response to infection by Colletotrichum falcatum Went. Acta Phytopathol Entomol Hungarica 31: 229–237.Google Scholar
  25. Viswanathan R, Malathi P, Ramesh Sundar A, Aarthi S, Premkumari SM, Padmanaban P (2005) Differential induction of chitinases and thaumatin-like proteins in sugarcane in response to infection by Colletotrichum falcatum causing red rot disease. J Plant Dis Prot 112: 417–425.Google Scholar
  26. Wharton PS and Nicholson RL (2000) Temporal synthesis and radiolabelling of the sorghum 3-deoxyanthocyanidin phytoalexins and the anthocyanin, cyaniding 3-dimalonyl glucoside. New Phytol 145: 457–469.CrossRefGoogle Scholar

Copyright information

© Society for Sugar Research & Promotion 2008

Authors and Affiliations

  • P. Malathi
    • 1
  • R. Viswanathan
    • 1
    Email author
  • P. Padmanaban
    • 1
  • D. Mohanraj
    • 1
  • V. Ganesh Kumar
    • 1
  • K. P. Salin
    • 1
  1. 1.Division of Crop Protection, Sugarcane Breeding InstituteICARCoimbatoreIndia

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