Is the effect of high fat diet on lipid and carbohydrate metabolism related to inflammation?
- 86 Downloads
Obesity is an emerging health problem in world wide countries. High fat diet (HFD), which is the main cause of obesity, induces inflammation and affects both lipid and carbohydrate metabolism. This study investigates the effect of inflammation induced by HFD on lipid and carbohydrate metabolism. This study involves two parts, in vivo and in vitro. In the in vivo part, adult male albino rats were fed a HFD for 8 and 14 weeks. After each feeding period, the following parameters were measured (body weight, blood glucose levels, oral glucose tolerance, serum insulin, insulin resistance, total cholesterol, HDLc, LDLc, triglycerides, free fatty acids, neutrophils percentage and serum CRP levels) in addition to pancreas tissue histopathology. In the in vitro part, insulin release from isolated perfused pancreas preparations was measured. Pancreas preparations were isolated from adult male albino rats fed a HFD for 16 weeks. Feeding rats a HFD for 8 and 14 weeks induced inflammation, impaired lipid profile and carbohydrate metabolism. Feeding rats a HFD for 16 weeks increased in vitro glucose-stimulated insulin release. HFD induces inflammation which plays an important role in the impairment of lipid and carbohydrate metabolism.
KeywordsDiabetes Insulin resistance Obesity Inflammation
Great thanks for all members of the pharmacology department, faculty of pharmacy, Zagazig university for scientific assistance and support. This research received no specific grant from any funding agency in the public, commercial or not-for-profit sectors.
Conflict of interest
There are no conflicts of interest.
- 2.Oakes ND, Bell KS, Furler SM et al (1997) Diet-induced muscle insulin resistance in rats is ameliorated by acute dietary lipid withdrawal or a single bout of exercise: parallel relationship between insulin stimulation of glucose uptake and suppression of long-chain fatty acyl-CoA. Diabetes 46:2022–2028CrossRefGoogle Scholar
- 6.Harris W (1997) n-3 fatty acids and serum lipoproteins: human studies. Am J Clin Nutr 65:1645–1654Google Scholar
- 7.Wong SH, Nestel PJ, Trimble RP et al (1984) The adaptive effects of dietary fish and safflower oil on lipid and lipoprotein metabolism in perfused rat liver. Biochim Biophys Acta 792:103–109Google Scholar
- 12.Tacikowski T, Nowicka G, Bujko J et al (2005) Effect of high-fat diet, rosiglitazone on lipid profile, insulin resistance and liver steatosis development. Pol Arch Med Wewn 113:213–222Google Scholar
- 13.Grodsky GM, Batts AA, Bennett LL et al (1963) Effects of carbohydrates on secretion of insulin from isolated rat pancreas. Am J Physiol 205:638–644Google Scholar
- 14.Trinder P (1969) Determination of glucose in blood using oxidase with alternative oxygen receptor. Ann Clin Biochem 6:24–27Google Scholar
- 15.Feldman H, Roadbard D (1971) Mathematical theory of radioimmunoassay. In: Odell WD, Doughaday WH (eds) Principles of competitive protein-binding assays. J.B. Leppincott company, Philadelphia, pp 158–203Google Scholar
- 18.Buccolo G, David H (1973) Quantitative determination of serum triglycerides by use of enzymes. Clin Chem 19:476–482Google Scholar
- 19.Allain GC, Poon LS, Chan CSG et al (1974) Quantitative determination of cholesterol using enzymatic colorimetric method. Clin Chem 20:470–475Google Scholar
- 20.Hoffman R, Benz EJ, Shattil SS et al (2005) Hematology: basic principles and practice, 4th edn. Churchill Livingstone, Orlando 1124–1126Google Scholar
- 21.Helgeson NGP, Adamson DM, Pike RB et al (1973) C-reactive protein: laboratory medicine. (2) Race GJ (ed). Harper and Row, Hagerstown (chapter 29)Google Scholar
- 22.Snedecor GW, Cochran WG (1967) Statistical methods. The Lowa state University Press, Ames, Lova, p 593Google Scholar
- 24.Furukawa S, Fujita T, Shimabukuro M et al (2004) Increased oxidative stress in obesity and its impact on metabolic syndrome. J Clin Invest 114(12):1752–1761Google Scholar
- 25.Festa A, D’Agostino R, Howard GG et al (2000) Chronic subclinical inflammation as part of the insulin resistance syndrome. The insulin resistance atherosclerosis study (IRAS). Circulation 102:42–47Google Scholar
- 27.Lepsanović L, Lepsanović L, Ivković-Lazar T et al (1997) Hyperinsulinemia as a key factor in the development of many metabolic disorders. Med Pregl 50:469–472Google Scholar
- 28.Feingold KR, Staprans I, Memon RA et al (1992) Endotoxin rapidly induces changes in lipid metabolism that produce hypertriglyceridemia: low doses stimulate hepatic triglyceride production while high doses inhibit clearance. J Lipid Res 33:1765–1776Google Scholar
- 29.Romanosky AJ, Bagby GJ, Bockman EL et al (1980) Free fatty acid utilization by skeletal muscle after endotoxin administration. Am J Physiol 239:E391–E395Google Scholar
- 30.Nonogaki K, Moser AH, Pan XM et al (1995) Lipoteichoic acid stimulates lipolysis and hepatic triglyceride secretion in rats in vivo. J Lipid Res 36:1987–1995Google Scholar
- 31.Hardardóttir I, Moser AH, Memon R et al (1994) Effects of TNF, IL-1, and the combination of both cytokines on cholesterol metabolism in Syrian hamsters. Lymphok Cytok Res 13:161–166Google Scholar